LOCUS       sequence-43284-e       11502 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Simonson WT, Franco SJ, Huttenlocher A
  TITLE     Talin1 regulates TCR-mediated LFA-1 function.
  JOURNAL   J Immunol. 2006 Dec 1;177(11):7707-14.
  PUBMED    17114441
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2006-12-1"; volume: "177(11)"; pages: "7707-14"
COMMENT     Sequence Label: EGFP-talin1 rod
FEATURES             Location/Qualifiers
     source          1..11502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8290..8411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8327..8346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8381..8400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8418..8873)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8555..8576)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8767..8786
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8900..9004
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9006..9363
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9006..9197
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9029..9049)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9214..9349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9398..10192
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9398..10192
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9452..9471)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10062..10081
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10380..10399)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10424..10471
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10800..11388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11289..11308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagctagct tcgaattcgc caccatgcag
     1381 cagtacaacc gagtggggaa agtggagcac ggctctgtgg ctctgccagc catcatgcgc
     1441 tctggagcct ctggtcctga gaatttccag gtggggagca tgccacctgc ccagcagcag
     1501 atcaccagtg gccagatgca ccgaggacac atgccacctc tgacttcagc ccagcaggcg
     1561 ctcactggaa ccattaactc cagcatgcag gctgtgcagg ctgcccaggc cactctggat
     1621 gactttgaga ctctaccccc tcttggccag gatgctgcct ccaaagcttg gcgtaagaac
     1681 aagatggatg aatcaaagca tgagatccac tcccaggtag atgccatcac agctggcact
     1741 gcctctgtgg taaacctgac agcaggagat cctgcagaga cagactatac agcagtgggc
     1801 tgtgcagtca ccaccatctc ttccaacctg acggagatgt cccgtggggt gaagctattg
     1861 gctgccctgc tggaggatga aggcggcaat ggccggcccc ttctgcaagc agcaaagggc
     1921 cttgcagggg ctgtgtcgga actgcttcgc agtgctcagc ccgccagcgc tgagcctcgt
     1981 cagaacctgc tgcaggcagc cgggaacgtg ggccaggcca gtggggagct gttgcagcaa
     2041 attggggaaa gtgacactga cccccacttc caggatgttc taatgcagct agcaaacgca
     2101 gtggcaagtg ctgcagccgc cctggtcctc aaggccaaga gtgtggccca gcgcacagag
     2161 gattccgggc ttcagaccca agtgattgct gcagctacgc aatgtgctct gtccacttcc
     2221 cagctggtgg cctgtaccaa ggtggtagca cctacaatca gctcacctgt gtgccaagaa
     2281 cagctagtcg aggctgggcg actggtggcc aaagctgtgg agggctgtgt gtctgcctcg
     2341 caagcagcta cagaagatgg acagctgctt cgaggggtag gagcagcagc cacggctgtc
     2401 acccaggccc tcaatgagct gctgcagcac gtgaaggccc acgccaccgg agctgggcct
     2461 gccggtcggt atgaccaagc cactgatacc atcctcaccg tcactgaaaa catcttcagc
     2521 tccatgggtg atgctgggga gatggtgcgc caggcccgca tcctggccca agccacctca
     2581 gacctggtca atgctatcaa ggccgatgct gagggggaga gtgatctgga gaactctaga
     2641 aagctcctga gtgctgccaa gatcctcgct gatgccaccg ccaagatggt ggaggcggcc
     2701 aagggagcag ccgcccaccc tgacagtgag gaacagcagc agcgactgcg tgaagcagct
     2761 gaggggcttc gcatggccac caatgcagct gcgcagaacg ccatcaagaa gaagttggtg
     2821 cagcgcctgg agcatgcagc caagcaagct gcagcctctg caacacagac cattgctgca
     2881 gcccaacatg cagcctctgc ccccaaggcc tctgcgggcc cccagcccct actggtgcag
     2941 agctgtaagg ccgtggcaga gcagattccg ttgctggtgc agggtgtccg aggaagtcaa
     3001 gctcagcctg acagccctag tgctcagctc gccctcattg ctgccagcca gagcttcctg
     3061 cagccaggtg ggaagatggt ggcagcggca aaagcctcag tgccaacaat tcaggaccag
     3121 gcttcagcga tgcagctgag tcagtgcgca aagaacctag gcactgccct ggccgagctg
     3181 cgcactgctg ctcagaaggc tcaggaggca tgtggacctt tggagatgga ttctgcactg
     3241 agtgttgtac aaaatctaga gaaagaccta caggaaataa aagcagcagc tcgagatggc
     3301 aagcttaagc ccttacctgg ggaaacaatg gagaagtgta cccaagatct tggcaacagc
     3361 accaaagcgg tgagctctgc catcgccaag ctgctggggg agattgccca gggcaatgag
     3421 aactatgcag gtattgcagc tcgggatgta gctggtggac taaggtcact agcccaggct
     3481 gcgcggggtg tggctgcgct gacatcagat cctgcagtgc aggccattgt gcttgacaca
     3541 gccagcgacg ttctggacaa ggccagcagc ctcattgaag aggcaaaaaa agcatctgga
     3601 cacccagggg acccagaaag ccagcaaagg cttgctcagg tagctaaagc agtgacccaa
     3661 gccctgaacc gctgtgtcag ctgcctgcct ggccaaagag acgtggataa tgccctacga
     3721 gccgttggag atgccagcaa gcgactcctg agtgacttgc ttcctccaag cacggggaca
     3781 tttcaagaag cacagagccg attgaatgaa gctgcagctg ggttaaatca ggccgccaca
     3841 gaactggtgc aagcctcccg aggaactcct caggacctgg ctcgggcctc cggccgattt
     3901 ggacaggact tcagcacctt cctggaagct ggtgtggaga tggccggaca ggccccgagc
     3961 caggaggacc gagcccaggt ggtgtccaac ctgaagggca tatccatgtc ttcaagcaaa
     4021 cttctccttg ccgctaaggc cttgtccaca gaccctgctt ctcccaacct caagagtcag
     4081 ctggctgcgg ctgcccgggc agtgacggac agcatcaacc agctcatcac catgtgcacc
     4141 cagcaggcac ctggccagaa ggagtgtgac aatgcacttc ggcagctaga gacagtccga
     4201 gaactcctgg agaatccagt ccagcccatc aacgacatgt cctacttcgg ttgcttggac
     4261 agtgtcatgg agaactccaa ggtcctaggt gaggccatga ctggcatctc ccaaaatgcc
     4321 aagaatggaa atctgccgga gtttggagat gccattgcca cagcctccaa ggctctctgt
     4381 ggcttcacgg aggcagccgc acaggcagca tatctagttg gtgtctctga ccccaacagc
     4441 caagctggac agcaaggact ggtggaaccc acacagtttg cccgtgcaaa ccaggcaatt
     4501 cagatggcct gtcagagtct gggggagcct ggctgtaccc aggcccaggt gttatctgca
     4561 gccactattg tagccaaaca cacatctgca ttgtgtaaca gctgtcgcct ggcttccgct
     4621 aggactgcca atcccactgc caagcgccag tttgtacagt cagccaagga ggtggccaac
     4681 agtacagcca atcttgtcaa gaccatcaag gcactagatg gggacttcac agaagagaac
     4741 cgtgcccagt gccgagcagc cacagcccct ctgctggaag ctgtggataa cctgagtgcc
     4801 tttgcctcca accctgagtt ctccagcgtc cctgcccaga tcagccctga gggcagagca
     4861 gccatggagc ccattgtaat ctctgctaag acaatgttgg agagtgctgg agggctcatc
     4921 cagacagctc gggcgttagc agtcaatccc cgagaccccc cacgttggtc tgtgctagct
     4981 ggccactctc ggactgtctc agattccatc aagaaactta ttacaagcat gagggacaaa
     5041 gccccagggc agctggagtg tgagacagcc attgcggctc tgaacagctg cttgcgggac
     5101 ctagatcagg cttcgcttgc cgctgtcagc cagcagcttg ctccccgtga aggaatctct
     5161 caagaggctt tgcacaccca gatgctcact gcagtgcaag aaatttctca tctcattgag
     5221 ccgctggcca gcgctgctcg ggctgaagcc tcccagctgg gacacaaggt gtcccaaatg
     5281 gcccagtact ttgaaccact caccctggct gcagtgggtg ctgcgtctaa gaccctgagc
     5341 cacccgcagc agatggcact tctggaccag actaaaacgt tggcagagtc tgccttgcag
     5401 ttgctatata ctgccaagga ggctggtggt aaccccaagc aagcagcaca cacccaggaa
     5461 gccctggagg aggctgtgca gatgatgaca gaggccgtag aagacctgac gacgaccctc
     5521 aacgaagcag ccagtgctgc aggagtcgtt ggcggcatgg tggactctat cactcaggcc
     5581 atcaaccagc tagatgaagg acctatgggt gacccagaag gctcattcgt agattaccag
     5641 acaaccatgg tgaggacagc caaggccatt gctgtcactg ttcaggagat ggtaaccaag
     5701 tcaaacacca gccctgaaga gctaggccct cttgccaacc agctgaccag tgactatggc
     5761 cgactggcct cacaagccaa gcctgcagct gtggctgctg aaaatgaaga gataggcgct
     5821 cacatcaaac accgagtaca ggagctgggc catggctgct ctgctctggt caccaaggca
     5881 ggtgccttgc agtgtagccc cagtgatgtc tacaccaaga aggagctcat agagtgtgcc
     5941 cgcagagtgt cagagaaggt ctcccatgtt ctggctgcac tccaggctgg gaatcgtggt
     6001 acccaggcct gcattacagc agccagtgct gtgtctggta tcattgctga cctcgacacc
     6061 accatcatgt tcgctactgc tggcacactt aaccgtgagg gtgctgaaac ttttgctgac
     6121 caccgggagg gtatcttaaa gacagcaaag gttcttgtgg aggacaccaa ggtcctagtg
     6181 cagaatgcag ctgggagcca ggagaagttg gcacaagccg cccagtcctc cgtggccacc
     6241 attacccgcc tcgctgatgt ggtcaagctc ggtgcagcca gcctaggagc cgaagaccct
     6301 gaaactcagg tggtgctgat caatgcagta aaggacgtag ccaaggccct gggtgacctc
     6361 atcagcgcta cgaaggctgc agcgggcaaa gttggggatg accctgcagt gtggcagctc
     6421 aagaactctg ccaaggtgat ggtgaccaat gtgacatcat tgctcaagac agtgaaggct
     6481 gtggaagatg aggccaccaa aggcacacgg gccctagagg caaccacaga gcacatacgt
     6541 caggaactgg cggtcttctg ttccccagag ccacctgcca agacctctac ccctgaagac
     6601 ttcatccgaa tgaccaaggg tattactatg gcaacagcca aagccgttgc tgctggcaat
     6661 tcctgtcgac aggaagatgt cattgccaca gccaatctga gccgacgggc tattgcggac
     6721 atgcttcggg cttgcaagga agcagctttc cacccagaag tggcgcctga tgtacggctc
     6781 cgagccctgc actatggccg ggagtgtgcc aatggttacc tggaactgct ggaccacgtg
     6841 ctgctgaccc ttcagaagcc aaacccagac ctgaagcagc agctaactgg acactcaaag
     6901 cgggttgctg gctctgtgac tgagctcatc caggctgctg aagccatgaa aggaacagag
     6961 tgggtggacc cagaggaccc tactgtcatt gctgagaatg agctcttggg agccgcagcc
     7021 gccatcgagg ctgcagccaa gaagctggag cagctgaagc ccagggccaa acccaaggag
     7081 gcggatgagt ccttgaactt tgaggaacaa atcctagaag ctgccaagtc catcgctgca
     7141 gccaccagtg cactggtaaa ggctgcgtca gcagcccaga gggagctggt ggctcaagga
     7201 aaggtgggcg ccattccagc caatgcactg gatgatgggc agtggtcgca gggcctcatt
     7261 tccgctgccc gtatggtggc tgcggccacc aacaatctgt gtgaagcagc taacgcagct
     7321 gtccagggcc atgctagcca ggagaaactc atttcctcag ccaagcaagt agctgcctcc
     7381 acagctcagc tcctggtagc ttgcaaggtc aaagcagatc aggactctga ggcaatgaaa
     7441 cggctccagg ctgctggcaa tgcagtgaag agggcctcag ataacctggt aaaggcggcc
     7501 cagaaagctg cagccttcga agaccaggag aatgagacgg tggtggtgaa ggagaaaatg
     7561 gttgggggca ttgcccagat tatcgcagca caggaagaga tgcttcggaa ggaacgagag
     7621 ctggaagagg ctcggaaaaa gctcgcccag atccggcagc agcagtacaa gttcttgcct
     7681 tcagagcttc gagacgagca ctaaaaaagc cctgtgtatt taatgcagac ccagcccaga
     7741 gactgtgcct gccactacca aagccttctg ggctgctcag gacccaagct gcccaacccc
     7801 agcactcccc caaagtgcct gccaaaccct gggcctggcc ctgcccagtc ccactgcaag
     7861 ccgtgtcctc tccctgactc ccaagtgcct ttgcacccta gggcccctaa gtgcctgccc
     7921 ccctccagag tattaacgct ccaagagtat tattaatgct gccgtacctc agtttgaacc
     7981 tgccagggcc ccagctgctc cagcctgcca gcagcttcca gccagtcccc acggccacgt
     8041 cagctcaact catccctttt tgatactata tcccctaccc agctacctat ggggcttgag
     8101 ggttgtaaac ccaaacaggt cagactccgg aattctgcag tcgacggtac cgcgggcccg
     8161 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     8221 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8281 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8401 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8461 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8521 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8581 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8641 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8701 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8761 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8821 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     8881 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8941 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9001 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9061 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9181 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9361 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     9421 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     9481 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     9541 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     9601 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     9661 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     9721 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     9781 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     9841 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     9901 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     9961 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    10021 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    10081 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    10141 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
    10201 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
    10261 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    10321 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
    10381 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    10441 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    10501 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    10561 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    10621 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
    10681 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10741 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10801 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10861 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10921 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    10981 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11041 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11101 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11161 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    11221 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    11281 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11341 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11401 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11461 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
