LOCUS       sequence-433151-       10441 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes SARS-CoV-2 spike R246-G252 deletion, D253N for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18
            deltaR246-G25...
FEATURES             Location/Qualifiers
     source          1..10441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3818..4406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3871..3891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4289..4300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4459..4580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4470..4489)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4524..4543
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4695..4741
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4771..4907
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4952..5023
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5068..5264
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5115..5250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5278..6072
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5332..5351)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5942..5961
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6083..6307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6503..7091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6583..6602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7837..7905,7906..8697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8055..8074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8710..8846
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8998..9069
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9074..9120)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9138..9186
                     /note="synthetic polyadenylation signal"
     enhancer        9267..9570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9572..9774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9725..9745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9908..10383
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata aatctggttc gggatctccc tcaaggcttc
      661 tccgcgttgg aacccctcgt ggacttgccg attggcatca acatcacccg gttccaaacc
      721 cttcttgccc ttcataacag tagtagtggt tggacagcgg gcgcagcagc ctactatgtg
      781 ggatatttgc aaccacgcac ctttcttctc aaatataacg aaaacggaac aattaccgat
      841 gccgtagact gtgccctcga tcccctgtcc gaaactaaat gcacactcaa gtccttcaca
      901 gttgagaaag gcatatacca aaccagtaac ttccgcgtcc aacccaccga atccattgtt
      961 cgattcccta acatcaccaa tttgtgtcct ttcggtgaag tatttaatgc cactcgattt
     1021 gcatcagttt atgcctggaa tcgcaagcga atatcaaatt gtgttgcaga ttatagtgtc
     1081 ctgtataata gcgcatcatt cagcactttt aagtgttatg gggtcagccc tacgaaattg
     1141 aatgacttgt gttttaccaa cgtttacgca gattctttcg tcatacgcgg cgacgaagtc
     1201 cgacagattg caccggggca aaccggaaag atagcagact ataattataa gttgccggat
     1261 gacttcaccg gctgcgttat agcatggaac tccaataatc tcgattcaaa agtcggcggc
     1321 aactataatt atctttaccg cctgtttcgc aagtcaaatc tgaagccctt tgaacgtgac
     1381 atctccaccg aaatttatca agctggcagc accccatgta atggagtgga agggtttaac
     1441 tgttattttc cccttcaatc atacggattt caaccaacaa atggggtggg ctatcaacca
     1501 tatcgggtgg tagtcttgag ctttgagctg ctccacgcac cggctacagt ttgcggaccg
     1561 aagaaaagca ctaacctggt gaagaataaa tgtgtgaact tcaactttaa tgggctcacc
     1621 ggaacagggg tgctgacaga atcaaacaag aaatttctcc cttttcaaca atttggtaga
     1681 gacattgccg ataccacaga tgccgtccgc gatccgcaaa cactggaaat cctcgatata
     1741 acaccctgta gcttcggcgg ggtcagcgtg ataactcccg gaacgaacac atccaaccaa
     1801 gtggctgtcc tgtatcaaga tgtcaactgt accgaagtcc ctgtggcgat acacgccgac
     1861 caactgacac ccacctggag agtctactca acaggttcca atgtctttca aactagagcg
     1921 ggttgtctca ttggcgctga gcatgtcaat aattcatacg aatgtgatat tcctattggg
     1981 gcaggaatct gtgcgtctta tcaaactcaa acgaactcac cacggcgcgc tcgctcagta
     2041 gccagccagt ccataatcgc ctataccatg agtctcgggg ctgagaactc cgtggcttat
     2101 tcaaacaatt ccatcgcaat cccaactaac tttaccatta gcgttaccac ggaaatcttg
     2161 cccgtcagca tgactaagac gagcgtagac tgtacgatgt atatctgtgg ggattccacc
     2221 gagtgtagca acctcttgct tcaatacggg tctttctgta ctcaactcaa ccgagcattg
     2281 accggaattg cggtcgaaca agacaagaat acccaagagg tgttcgcaca agtaaagcaa
     2341 atatacaaaa cgccgccaat caaagatttt ggcgggttta attttagcca aatactgcca
     2401 gacccatcta agccttcaaa acgcagcttt atcgaggatc tgctgttcaa caaagttacg
     2461 ttggctgacg cgggtttcat taagcagtat ggggattgtt tgggagacat tgcggcccgc
     2521 gaccttatct gtgctcaaaa attcaatggg ttgacagttc tgccccctct gctcactgat
     2581 gagatgattg cacagtatac atcagcattg ttggcaggaa ccattacatc cggctggact
     2641 tttggcgcgg gtgcggccct tcaaatacct ttcgctatgc aaatggctta cagattcaat
     2701 gggattggtg tcacgcaaaa tgtattgtat gaaaatcaga agctgatcgc taatcaattt
     2761 aacagcgcga ttggcaagat ccaagattca ctgtcaagca cagcgtccgc cctcggcaaa
     2821 ctccaagatg ttgtgaatca aaacgcccag gccctgaata ccctcgtgaa gcaactgagt
     2881 tctaacttcg gcgcaatctc cagcgtcctc aacgacatat tgtccagact tgataaggtg
     2941 gaagccgagg tgcaaattga tcggctcatc acaggcaggc tccagtcact ccaaacttat
     3001 gtgacacaac agttgatccg ggctgctgag atacgagcaa gcgccaacct ggccgccact
     3061 aaaatgtcag agtgcgtgct cgggcagtcc aaacgggttg acttttgtgg caagggctat
     3121 cacttgatgt ccttccccca atccgcccca catggagtgg tctttctcca cgtcacttat
     3181 gtccctgctc aagagaagaa ctttacgaca gctccagcaa tctgtcacga tggcaaagcg
     3241 cactttccca gggaaggcgt tttcgtcagt aatggcacgc attggtttgt gacccaaagg
     3301 aatttctatg aaccccaaat cataaccact gacaatacat ttgtctcagg caactgtgat
     3361 gtggtcattg ggatcgtgaa caacacggta tatgatcccc tgcaacccga gttggatagc
     3421 ttcaaagagg aattggataa atactttaag aatcatactt cacccgatgt tgatctcggg
     3481 gacatatcag gaataaatgc aagtgtcgtc aacattcaga aagagattga tcgccttaat
     3541 gaggtcgcca agaacttgaa tgaatctttg atcgacttgc aagaactggg caaatacgaa
     3601 caatatatta aatggccctg gtacatttgg ttgggcttta tcgccggact tatagctatc
     3661 gttatggtca caattatgtt gtgctgtatg actagctgtt gttcctgctt gaaaggatgt
     3721 tgcagttgcg gatcttgttg taaataaagg atcctctaga agttgtctcc tcctgcactg
     3781 actgactgat acaatcgatt tctggatccg caggcctaat caacctctgg attacaaaat
     3841 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3901 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3961 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4021 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4081 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4141 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4201 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4261 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4321 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4381 gatctccctt tgggccgcct ccccgcctgt ctagcttgac tgactgagat acagcgtacc
     4441 ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4501 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4561 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4621 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat
     4681 ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     4741 gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg
     4801 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa
     4861 cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat
     4921 cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc
     4981 actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt
     5041 gggctatcga aacttaatta agcgatctgc atctcaatta gtcagcaacc atagtcccgc
     5101 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc
     5161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaggaggt agccaacatg
     5281 attgaacaag atggattgca cgcaggttct cccgccgctt gggtggagag gctattcggc
     5341 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5401 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccag
     5461 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5521 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5581 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5641 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5701 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5761 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc
     5821 gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5881 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5941 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6001 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6061 gagttcttct agtatgtaag ccctgtgcct tctagttgcc agccatctgt tgtttgcccc
     6121 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6181 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6241 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6301 tctatggtta attaaccagt caagtcagct acttggcgag atcgacttgt ctgggtttcg
     6361 actacgctca gaattgcgtc agtcaagttc gatctggtcc ttgctattgc acccgttctc
     6421 cgattacgag tttcatttaa atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7321 tagttgcatt taaatttccg aactctccaa ggccctcgtc ggaaaatctt caaacctttc
     7381 gtccgatcca tcttgcaggc tacctctcga acgaactatc gcaagtctct tggccggcct
     7441 tgcgccttgg ctattgcttg gcagcgccta tcgccaggta ttactccaat cccgaatatc
     7501 cgagatcggg atcacccgag agaagttcaa cctacatcct caatcccgat ctatccgaga
     7561 tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga gaacgatcct ctcagtgcga
     7621 gtctcgacga tccatatcgt tgcttggcag tcagccagtc ggaatccagc ttgggaccca
     7681 ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac ggcagcgtac cgatctgttt
     7741 aaacctagat attgatagtc tgatcggtca acgtataatc gagtcctagc ttttgcaaac
     7801 atctatcaag agacaggatc agcaggaggc tttcgcatga gtattcaaca tttccgtgtc
     7861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7921 gtgaaagtaa aagatgctga agatcagttg ggtgcgcgag tgggttacat cgaactggat
     7981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgctttcc aatgatgagc
     8041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa
     8161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8221 gataacactg cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct
     8281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaaccttg
     8401 cgtaaactat taactggcga actacttact ctagcttccc ggcaacagtt gatagactgg
     8461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaccg
     8701 attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc
     8761 cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt
     8821 cctccttacc agaaatttat ccttaagatc ccgaatcgtt taaactcgac tctggctcta
     8881 tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc
     8941 atcgaatctc gtcagctatc gtcagcttac ctttttggca gcgatcgcgg ctcccgacat
     9001 cttggaccat tagctccaca ggtatcttct tccctctagt ggtcataaca gcagcttcag
     9061 ctacctctca attcaaaaaa cccctcaaga cccgtttaga ggccccaagg ggttatgcta
     9121 tcaatcgttg cgttacacac acaaaaaacc aacacacatc catcttcgat ggatagcgat
     9181 tttattatct aactgctgat cgagtgtagc cagatctagt aatcaattac ggggtcatta
     9241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     9541 atctacgtat tagtcatcgc tattaccatg ctgatgcggt tttggcagta catcaatggg
     9601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     9661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     9721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     9781 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     9841 cgggaccgat ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa
     9901 cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag
     9961 ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt
    10021 ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat
    10081 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
    10141 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
    10201 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
    10261 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
    10321 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
    10381 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa aggcggccgc
    10441 c
//
