LOCUS       sequence-433155-        9948 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encoding mSOS1-linkercat fused to miRFP703.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  TITLE     Collective cell migration is coordinated by front-biased activation 
            of the feed-forward loop consisting of Ras, Rab5, and Rac1.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPBpuro-miRFP703-eDHFR(69K6)-mSOS1-linkercat
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1669
                     /label=chicken beta-actin promoter
     intron          1670..2686
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2610..2632
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2694..2713
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2777..2786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2783..3727
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    5854..6403
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5998..6015)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6225..6244
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6406..7005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6406..6425)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6902..6922
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7032..7049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7038..7262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7309..7325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7333..7349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7357..7363,7364..7381,7382..7387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7402..7423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7657..7719
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7760..7780)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7760..7778)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7799..7815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7823..7839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7847..7853,7854..7871,7872..7877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7892..7913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8030..8047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8201..8789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8281..8300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8960..9751,9752..9820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9583..9602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9821..9925)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     1561 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1621 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1681 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1741 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     1801 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     1861 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1921 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     1981 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     2041 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2101 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     2161 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     2221 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     2281 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     2341 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     2401 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     2461 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     2521 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     2581 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     2641 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     2701 gctggttatt gtgctgtctc atcattttgg caaagaattg ggatcctcac gtcaattgta
     2761 gctagcgcta ccggtcgcca ccatggtagc aggtcatgcc tctggcagcc ccgcattcgg
     2821 gaccgcctct cattcgaatt gcgaacatga agagatccac ctcgccggct cgatccagcc
     2881 gcatggcgcg cttctggtcg tcagcgaaca tgatcatcgc gtcatccagg ccagcgccaa
     2941 cgccgcggaa tttctgaatc tcggaagcgt actcggcgtt ccgctcgccg agatcgacgg
     3001 cgatctgttg atcaagatcc tgccgcatct cgatcccacc gccgaaggca tgccggtcgc
     3061 ggtgcgctgc cggatcggca atccctctac ggagtactgc ggtctgatgc atcggcctcc
     3121 ggaaggcggg ctgatcatcg aactcgaacg tgccggcccg tcgatcgatc tgtcaggcac
     3181 gctggcgccg gcgctggagc ggatccgcac ggcgggttca ctgcgcgcgc tgtgcgatga
     3241 caccgtgctg ctgtttcagc agtgcaccgg ctacgaccgg gtgatggtgt atcgtttcga
     3301 tgagcaaggc cacggcctgg tattctccga gtgccatgtg cctgggctcg aatcctattt
     3361 cggcaaccgc tatccgtcgt cgctggtccc gcagatggcg cggcagctgt acgtgcggca
     3421 gcgcgtccgc gtgctggtcg acgtcaccta tcagccggtg ccgctggagc cgcggctgtc
     3481 gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc ctgcgctcga tgtcgccgat
     3541 ccatctgcag ttcctgaagg acatgggcgt gcgcgccacc ctggcggtgt cgctggtggt
     3601 cggcggcaag ctgtggggcc tggttgtctg tcaccattat ctgccgcgct tcatccgttt
     3661 cgagctgcgg gcgatctgca aacggctcgc cgaaaggatc gcgacgcgga tcaccgcgct
     3721 tgagagcctg tacaagtctg ggtccgcagc cagtagcgcc ggaagtatga tcagtctgat
     3781 tgcggcgtta gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc
     3841 tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca
     3901 tacctgggaa tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca
     3961 accgggtacg gacaaaaaaa agaaaaagaa agatcgcgta acgtgggtga agtcggtgga
     4021 tgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt
     4081 ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg acgcagaagt
     4141 ggaaggcgac acccatttcc cggattacga gccggatgac tgggaatcgg tattcagcga
     4201 attccacgat gctgatgcgc agaactctca cagctattgc tttgagattc tggagcggcg
     4261 gagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     4321 tcctcgaagc ctcgagaccc tggagaggat gctggacgta acggtgctgc aggaggagaa
     4381 ggaggagcag atgaggctgc ccagtgctga agtgtacagg tttgcagaac ctgactccga
     4441 ggagaatatt ctattcgaag agaatgtgca gcccaaagct gggatcccca ttatcaaggc
     4501 agggacagtg cttaagctca ttgagaggct tacctaccac atgtacgcag atccaaattt
     4561 tgttcggacg tttcttacaa catacaggtc cttttgcaga cctcaagaac tactgagtct
     4621 tctgatagaa agatttgaaa ttccagagcc tgagccaaca gaagctgatc gcatagctat
     4681 agagaatgga gatcagcccc tgagtgcaga gctgaagagg tttagaaagg aatatattca
     4741 gcctgtgcag ttgagggtgt taaatgtgtg tcggcactgg gtggagcacc atttctatga
     4801 ctttgaaaga gatgcagacc ttttacagag aatggaggaa tttattggaa cagtaagagg
     4861 taaagcaatg aaaaaatggg tcgaatccat cactaagata atccaaagga aaaaaattgc
     4921 aagagacaat ggcccaggtc ataacattac atttcagagc tcacctccca cagttgagtg
     4981 gcacataagc agacctgggc acatagagac ttttgacttg ctcaccttac acccaataga
     5041 aattgctcgg caactcactt tacttgaatc agatctatac cgggctgtgc agccatcaga
     5101 attagttgga agtgtgtgga caaaagaaga taaagaaatt aattctccca accttctgaa
     5161 gatgattcgg cacaccacta acctcacttt gtggtttgag aaatgtattg tagaaacaga
     5221 aaacttagaa gaaagagtag ctgtagtaag tcggataatt gagattctac aagtctttca
     5281 agagctgaac aacttcaatg gtgtcctgga agttgtcagt gctatgaact cgtcacctgt
     5341 ttacagacta gaccacacat ttgagcaaat accaagcaga caaaagaaaa ttttagaaga
     5401 agctcatgaa ttgagtgaag atcactataa gaaatatttg gcaaaactca ggtctattaa
     5461 tccaccgtgt gtgcctttct ttggaattta tctcacaaat atcctgaaga cagaagaggg
     5521 caaccctgag gtcctgagga gacacgggaa agagcttatt aacttcagca agaggaggag
     5581 agtggccgag atcacaggcg agatccagca gtaccagaac cagccctact gcttacgggt
     5641 ggagccggac atcaagaggt tctttgaaaa cttgaatcca atgggaaaca gcatggagaa
     5701 agaatttaca gactatctgt tcaacaaatc cctagaaata gaaccccggc acggcggccg
     5761 ctagtcgacg gtaccgcggg cccgggataa gtcaactaac ttaagctagc aacggtttcc
     5821 ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     5881 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5941 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     6001 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     6061 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     6121 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     6181 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     6241 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6301 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     6361 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgac cgagtacaag
     6421 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6481 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6541 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6601 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6661 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6721 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6781 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6841 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6901 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6961 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatcgat gatctagagc
     7021 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     7081 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7141 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7201 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7261 ggcttctgag gcggaaagaa cctgcagccc aagcttggcg taatcatggt catagctgtt
     7321 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7381 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7441 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc ttaaaagttt tgttacttta
     7501 tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt
     7561 tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat
     7621 taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt
     7681 atctttaacg tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgttta
     7741 attaatccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     7801 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7861 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7921 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7981 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
