LOCUS       sequence-433656-       11388 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dox inducible WT1 cDNA - as transcribed from +378ATG (short 
            isoform).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki 
            N, Heidenreich O, Cockerill PN, Bonifer C
  TITLE     Isoform-specific and signaling-dependent propagation of acute 
            myeloid leukemia by Wilms tumor 1.
  JOURNAL   Cell Rep. 2021 Apr 20;35(3):109010. doi: 
            10.1016/j.celrep.2021.109010.
  PUBMED    33882316
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-04-20"; volume: "35(3):109010. doi"; pages: " 
            10.1016/j.celrep.2021.109010"
COMMENT     Sequence Label: pCW57.1 WT1 +378 ATG
FEATURES             Location/Qualifiers
     source          1..11388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             979..990
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2864..2888)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2911..3478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2928..3480
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3072..3089)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3299..3318
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3492..3494,3495..3497,3498..4211)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3537..3558)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3798..3817)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4145..4166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4261..4285)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4303..4320
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4330..4840
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4759..4777
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4850..5446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4850..4869)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5346..5366
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5447..5500
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5501..6118,6119..6124,6125..6160,6161..6163,6164..6199,
                     6200..6202,6203..6238,6239..6247)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6287..6307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6705..6716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6921..7154
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7226..7347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7263..7282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7317..7336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7387..7522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7522..7593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7594..7662,7663..8454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7812..7831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8625..9213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9114..9133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9367..9384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9501..9522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9537..9542,9543..9560,9561..9567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9575..9591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9580..9602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9599..9615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9599..9615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9634..9654
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9636..9654
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9682..9908
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9909..10089
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10136..10261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10754..10987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11172..11216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accaattcag tcgacgccac
      601 catgtttcct aacgcgccct acctgcccag ctgcctcgag agccagcccg ctattcgcaa
      661 tcagggttac agcacggtca ccttcgacgg gacgcccagc tacggtcaca cgccctcgca
      721 ccatgcggcg cagttcccca accactcatt caagcatgag gatcccatgg gccagcaggg
      781 ctcgctgggt gagcagcagt actcggtgcc gcccccggtc tatggctgcc acacccccac
      841 cgacagctgc accggcagcc aggctttgct gctgaggacg ccctacagca gtgacaattt
      901 ataccaaatg acatcccagc ttgaatgcat gacctggaat cagatgaact taggagccac
      961 cttaaaggga gttgctgctg ggagctccag ctcagtgaaa tggacagaag ggcagagcaa
     1021 ccacagcaca gggtacgaga gcgataacca cacaacgccc atcctctgcg gagcccaata
     1081 cagaatacac acgcacggtg tcttcagagg cattcaggat gtgcgacgtg tgcctggagt
     1141 agccccgact cttgtacggt cggcatctga gaccagtgag aaacgcccct tcatgtgtgc
     1201 ttacccaggc tgcaataaga gatattttaa gctgtcccac ttacagatgc acagcaggaa
     1261 gcacactggt gagaaaccat accagtgtga cttcaaggac tgtgaacgaa ggttttctcg
     1321 ttcagaccag ctcaaaagac accaaaggag acatacaggt gtgaaaccat tccagtgtaa
     1381 aacttgtcag cgaaagttct cccggtccga ccacctgaag acccacacca ggactcatac
     1441 aggtgaaaag cccttcagct gtcggtggcc aagttgtcag aaaaagtttg cccggtcaga
     1501 tgaattagtc cgccatcaca acatgcatca gagaaacatg accaaactcc agctggcgct
     1561 ttgaggggtc tccctcgggg accgttcagt gtcccaggca gcacagtgtg tgaactgctt
     1621 tcaagtctga ctctccactc ctcctcacta aaaaggaaac ttcagttgat cttcttcatc
     1681 caacttccaa gacaagatac cggtgcttct ggaaactacc aggtgtgcct ggaagagttg
     1741 gtctctgccc tgcctacttt tagttgactc acaggccctg gagaagcagc taacaatgtc
     1801 tggttagtta aaagcccatt gccatttggt gtggattttc tactgtaaga agagccatag
     1861 ctgatcatgt ccccctgacc cttcccttct ttttttatgc tcgttttcgc tggggatgga
     1921 attattgtac cattttctat catggaatat ttataggcca gggcatgtgt atgtgtctgc
     1981 taatgtaaac tttgtcatgg tttccattta ctaacagcaa cagcaagaaa taaatcagag
     2041 agcaaggcat cgggggtgaa tcttgtctaa cattcccgag gtcagccagg ctgctaacct
     2101 ggaaagcagg atgtagttct gccaggcaac ttttaaagct catgcatttc aagcagctga
     2161 agaaagaatc agaactaacc agtacctctg tatagaaatc taaaagaatt ttaccattca
     2221 gttaattcaa tgtgaacact ggcacactgc tcttaagaaa ctatgaagat ctgagatttt
     2281 tttgtgtatg tttttgactc ttttgagtgg taatcatatg tgtctttata gatgtacata
     2341 cctccttgca caaacggagg ggaattcatt ttcatcactg ggagtgtcct tagtgtataa
     2401 aaaccatgct ggtatatggc ttcaagttgt aaaaatgaaa gcgactttaa aagaaaatag
     2461 gggatggtcc aggatctcca ctgataagac tgtttttaag taacttaagg acctttgggt
     2521 ctacaagtat atgtgaaaaa aatgagactt actgggtgag gaaatccatt gtttaaagat
     2581 ggtcgtgtgt gtgtgtgtgt gtgtgtgttg tgttgtgttt tgttttttaa gggagggagt
     2641 ttattattta ccgttgcttg aaattactgt gtaaatatat gtctgataat gatttgctct
     2701 ttgacaacta aaattaggac tgtataagta ctagatgcat cactgggtgt tgatcttaca
     2761 agatattgat gataacactt aaaattgtaa cctgcatttt tcactttgct ctcaattaaa
     2821 gtctattcaa aaggtaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac
     2881 aaagtggtga ttcccccggg ctgcaggaat tccgcccccc ccccctaacg ttactggccg
     2941 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3001 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3061 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3121 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3181 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3241 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3301 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3361 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3421 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3481 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3541 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg
     3601 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3661 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3721 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3781 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3841 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3901 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3961 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg
     4021 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4081 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4141 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4201 tgtacaagta atgaattaat taagaattat caagcttatc gataccgtcg agatatctag
     4261 acccagcttt cttgtacaaa gtggtttagt aatgaaccgg tccaccacca ccaccaccac
     4321 taaggatccg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     4381 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     4441 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     4501 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     4561 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     4621 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     4681 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     4741 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     4801 cggctccctc gttgaccgaa tcaccgacct ctctccccag caattcacca tgaccgagta
     4861 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4921 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4981 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5041 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5101 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5161 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5221 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5281 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5341 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5401 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc
     5461 tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca atgtctagac tggacaagag
     5521 caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac
     5581 aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa
     5641 caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt
     5701 ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg
     5761 ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga
     5821 gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct
     5881 ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga
     5941 ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc
     6001 cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct
     6061 tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg
     6121 gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga
     6181 ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc
     6241 cgggtaacta agtaaggatc gatccaagat atcgtattct taactatgtt gctcctttta
     6301 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6361 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6421 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6481 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6541 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6601 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6661 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6721 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6781 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc
     6841 cggactagag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6901 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6961 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7021 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7081 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7141 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7201 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7321 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7381 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7441 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7501 tgaggaggct tttttggagg cctttcaaat atgtatccgc tcatgagaca ataaccctga
     7561 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7621 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7681 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7741 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7801 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7861 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7921 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7981 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8041 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8101 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8161 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8221 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8281 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8341 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8401 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8461 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8521 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8581 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8641 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8701 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8761 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8821 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8881 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8941 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9001 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9061 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9481 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9541 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9601 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9661 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     9721 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     9781 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9841 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9901 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9961 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10021 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10081 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    10141 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    10201 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    10261 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    10321 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    10381 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    10441 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    10501 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    10561 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    10621 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10681 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10741 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10801 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10861 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10921 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10981 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    11041 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    11101 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    11161 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    11221 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    11281 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    11341 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggac
//
