LOCUS       sequence-43389-e       10006 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  TITLE     pCMV-STAT6-IRES-Neo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-STAT6-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(745..813,814..1605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(963..982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    1707..1740
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1818..2406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2307..2326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2560..2577
                     /label=L4440
                     /note="L4440 vector, forward primer"
     enhancer        2866..3169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3170..3373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3324..3344
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3370..3394
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3480..3502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3499..3515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3499..3515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3526..3544
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3526..3543
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    3545..3569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7524..7753
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7736..7755
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7811..8385,8386..8388,8389..8397)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7978..7995)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8205..8224
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8420..9223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8483..8502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9093..9112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(9272..9296)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9297..9316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9298..9316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9327..9344)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9327..9343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9336..9358)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    9748..9882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9785..9804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9839..9858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 ccaggtcttg gactggtgag aacggcttgc tcggcagctt cgatgtgtgc tggagggaga
      601 ataaaggtct aagatgtgcg atagagggaa gtcgcattga attatgtgct gtgtagggat
      661 cgctggtatc aaatatgtgt gcccacccct ggcatgagac aataaccctg ataaatgctt
      721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1021 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1081 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1141 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1201 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1261 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1321 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1381 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1441 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1501 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1561 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1621 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1681 aagatccttt ttgataatct catgccataa cttcgtataa tgtatgctat acgaagttat
     1741 ggcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2641 agagcttgca attcgcgcgt ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2821 aaaaataggc gtagtacgag gccctttcac tcattagatg catgtcgtta cataacttac
     2881 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2941 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3001 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3061 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3121 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3181 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3241 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3301 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3361 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3421 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcct aggccgcgga
     3481 gcggataaca atttcacaca ggaaacagct atgaccatta ggcctattta ggtgacacta
     3541 tagaacaagt ttgtacaaaa aagcaggctg gtaccggtcc ggaattcccg ggatatcgtc
     3601 gacccacgcg tccgcttgaa ctcgctggac agagctacag acctatgggg cctggaagtg
     3661 cccgctgaga aagggagaag acagcagagg ggttgccgag gcaacctcca agtcccagat
     3721 catgtctctg tggggtctgg tctccaagat gcccccagaa aaagtgcagc ggctctatgt
     3781 cgactttccc caacacctgc ggcatcttct gggtgactgg ctggagagcc agccctggga
     3841 gttcctggtc ggctccgacg ccttctgctg caacttggct agtgccctac tttcagacac
     3901 tgtccagcac cttcaggcct cggtgggaga gcagggggag gggagcacca tcttgcaaca
     3961 catcagcacc cttgagagca tatatcagag ggaccccctg aagctggtgg ccactttcag
     4021 acaaatactt caaggagaga aaaaagctgt tatggaacag ttccgccact tgccaatgcc
     4081 tttccactgg aagcaggaag aactcaagtt taagacaggc ttgcggaggc tgcagcaccg
     4141 agtaggggag atccaccttc tccgagaagc cctgcagaag ggggctgagg ctggccaagt
     4201 gtctctgcac agcttgatag aaactcctgc taatgggact gggccaagtg aggccctggc
     4261 catgctactg caggagacca ctggagagct agaggcagcc aaagccctag tgctgaagag
     4321 gatccagatt tggaaacggc agcagcagct ggcagggaat ggcgcaccgt ttgaggagag
     4381 cctggcccca ctccaggaga ggtgtgaaag cctggtggac atttattccc agctacagca
     4441 ggaggtaggg gcggctggtg gggagcttga gcccaagacc cgggcatcgc tgactggccg
     4501 gctggatgaa gtcctgagaa ccctcgtcac cagttgcttc ctggtggaga agcagccccc
     4561 ccaggtactg aagactcaga ccaagttcca ggctggagtt cgattcctgt tgggcttgag
     4621 gttcctgggg gccccagcca agcctccgct ggtcagggcc gacatggtga cagagaagca
     4681 ggcgcgggag ctgagtgtgc ctcagggtcc tggggctgga gcagaaagca ctggagaaat
     4741 catcaacaac actgtgccct tggagaacag cattcctggg aactgctgct ctgccctgtt
     4801 caagaacctg cttctcaaga agatcaagcg gtgtgagcgg aagggcactg agtctgtcac
     4861 agaggagaag tgcgctgtgc tcttctctgc cagcttcaca cttggccccg gcaaactccc
     4921 catccagctc caggccctgt ctctgcccct ggtggtcatc gtccatggca accaagacaa
     4981 caatgccaaa gccactatcc tgtgggacaa tgccttctct gagatggacc gcgtgccctt
     5041 tgtggtggct gagcgggtgc cctgggagaa gatgtgtgaa actctgaacc tgaagttcat
     5101 ggctgaggtg gggaccaacc gggggctgct cccagagcac ttcctcttcc tggcccagaa
     5161 gatcttcaat gacaacagcc tcagtatgga ggccttccag caccgttctg tgtcctggtc
     5221 gcagttcaac aaggagatcc tgctgggccg tggcttcacc ttttggcagt ggtttgatgg
     5281 tgtcctggac ctcaccaaac gctgtctccg gagctactgg tctgaccggc tgatcattgg
     5341 cttcatcagc aaacagtacg ttactagcct tcttctcaat gagcccgacg gaacctttct
     5401 cctccgcttc agcgactcag agattggggg catcaccatt gcccatgtca tccggggcca
     5461 ggatggctct ccacagatag agaacatcca gccattctct gccaaagacc tgtccattcg
     5521 ctcactgggg gaccgaatcc gggatcttgc tcagctcaaa aatctctatc ccaagaagcc
     5581 caaggatgag gctttccgga gccactacaa gcctgaacag atgggtaagg atggcagggg
     5641 ttatgtccca gctaccatca agatgaccgt ggaaagggac caaccacttc ctaccccaga
     5701 gctccagatg cctaccatgg tgccttctta tgaccttgga atggcccctg attcctccat
     5761 gagcatgcag cttggcccag atatggtgcc ccaggtgtac ccaccacact ctcactccat
     5821 ccccccgtat caaggcctct ccccagaaga atcagtcaac gtgttgtcag ccttccagga
     5881 gcctcacctg cagatgcccc ccagcctggg ccagatgagc ctgccctttg accagcctca
     5941 cccccagggc ctgctgccgt gccagcctca ggagcatgct gtgtccagcc ctgaccccct
     6001 gctctgctca gatgtgacca tggtggaaga cagctgcctg agccagccag tgacagcgtt
     6061 tcctcagggc acttggattg gtgaagacat attccctcct ctgctgcctc ccactgaaca
     6121 ggacctcact aagcttctcc tggaggggca aggggagtcg gggggagggt ccttgggggc
     6181 acagcccctc ctgcagccct cccactatgg gcaatctggg atctcaatgt cccacatgga
     6241 cctaagggcc aaccccagtt ggtgatccca gctggaggga gaacccaaag agacagctct
     6301 tctactaccc ccacagacct gctctggaca cttgctcatg ccctgccaag cagcagatgg
     6361 ggagggtgcc ctcctatccc cacctactcc tgggtcagga ggaaaagact aacaggagaa
     6421 tgcacagtgg gtggagccaa tccactcctt cctttctatc attcccctgc ccacctcctt
     6481 ccagcactga ctggaaggga agttcaggct ctgagacacg ccccaacatg cctgcacctg
     6541 cagcgcgcac acgcacgcac acacacatac agagctctct gagggtgatg gggctgagca
     6601 ggaggggggc tgggtaagag cacaggttag ggcatggaag gcttctccgc ccattctgac
     6661 ccagggccta ggacggatag gcaggaacat acagacacat ttacactaga ggccagggat
     6721 agaggatatt gggtctcagc cctaggggaa tgggaagcag ctcaagggac cctgggtggg
     6781 agcataggag gagtctggac atgtggttac tagtacaggt tttgccctga ttaaaaaatc
     6841 tcccaaagcc ccaaattcct gttagccagg tggaggcttc tgatacgtgt atgagactat
     6901 gcaaaagtac aagggctgag attcttcgtg tatagctgtg tgaacgtgta tgtacctagg
     6961 atatgttaaa tatatagctg gcaccttagt tgcatgacca catagaacat gtgtctatct
     7021 gcttttgcct acgtgacaac acaaatttgg gagggtgaga cactgcacag aagacagcag
     7081 caagtgtgct ggcctctctg acatatgcta acccccaaat actctgaatt tggagtctga
     7141 ctgtgcccaa gtgggtccaa gtggctgtga catctacgta tggctccaca cctccaatgc
     7201 tgcctgggag ccagggtgag agtctgggtc caggcctggc catgtggccc tccagtgtat
     7261 gagagggccc tgcctgctgc atcttttctg ttgccccatc caccgccagc ttcccttcac
     7321 tcccctatcc cattctccct ctcaaggcag gggtcataga tcctaagcca taaaataaat
     7381 tttattccaa aataacaaaa taaataatct actgtacaca aaaaaaaaaa aaaaaaaaaa
     7441 aaaaaaaaaa aaggggcggc cgcatagata actgatccag tgtgctggaa ttaattcgct
     7501 gtctgcgagg gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc
     7561 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat
     7621 gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt
     7681 gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc
     7741 tttctctcca caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg
     7801 gccaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     7861 aaggccggtg tgcgtttgtc tatatgtgat tttccaccat attgccgtct tttggcaatg
     7921 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     7981 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     8041 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     8101 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     8161 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     8221 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     8281 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     8341 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg
     8401 gataattcct gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc
     8461 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     8521 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     8581 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     8641 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     8701 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     8761 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     8821 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     8881 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     8941 cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc
     9001 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     9061 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     9121 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     9181 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc aattctctag
     9241 agtatccctc gaggggccca agcttacgcg tacccagctt tcttgtacaa agtggtccct
     9301 atagtgagtc gtattataag ctaggcactg gccgtcgttt tacaacgtcg tgactgggaa
     9361 aactgctagc ttgggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     9421 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     9481 gttaaactag ctgcatatgc ttgctgcttg agagttttgc ttactgagta tgatttatga
     9541 aaatattata cacaggagct agtgattcta attgtttgtg tattttagat tcacagtccc
     9601 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
     9661 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     9721 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     9781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9841 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatcctgc attaatgaat
     9901 cggccaacgc gcggggagag gcggtttgcg tattggctgg cgtaatagcg aagaggcccg
     9961 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgg
//
