LOCUS       sequence-434227-        9501 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses C-terminally EGFP-tagged IFT122 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9501)
  TITLE     Nakayama Lab Plasmids 2024
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG2-EGFP-C-IFT122
FEATURES             Location/Qualifiers
     source          1..9501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..660
                     /label=chicken beta-actin promoter
     intron          661..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1733..1742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1784..1805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2045..2064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2392..2413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6167..6186)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6232..6287
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6286..6305)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6642..6661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6643..6661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6671..6688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6671..6687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6680..6702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6829..7284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6916..6935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7126..7147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7310..7414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7415..7483,7484..8275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7633..7652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8446..9034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8935..8954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9188..9205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9322..9343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9358..9363,9364..9381,9382..9388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9396..9412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9401..9423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9420..9436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9420..9436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9455..9475
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9457..9475
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaattaccgg tcgccaccat
     1741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1801 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2041 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2161 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2221 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2341 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     2461 actcagatct cgagctcaag cttcgaattc tatgagggcc gtgttgacgt ggagagataa
     2521 agccgagcac tgtataaatg acatcgcatt taagcctgat ggaactcaac tgattttggc
     2581 tgccggaagc agattactgg tttatgacac ctctgatggc accttacttc agcccctcaa
     2641 gggacacaaa gacactgtgt actgtgtggc atatgcgaag gatggcaagc gctttgcttc
     2701 tggatcagct gacaaaagcg ttattatctg gacatcaaaa ctggaaggca ttctgaagta
     2761 cacgcacaat gatgctatac aatgtgtctc ctacaatcct attactcatc aactggcatc
     2821 ttgttcctcc agtgactttg ggttgtggtc tcctgaacag aagtctgtct ccaaacacaa
     2881 atcaagcagc aagatcatct gctgcagctg gacaaatgat ggtcagtacc tggcgctggg
     2941 gatgttcaat gggatcatca gcatacggaa caaaaatggc gaggagaaag taaagatcga
     3001 gcggccgggg ggctccctct cgccaatatg gtccatctgc tggaaccctt caagagagga
     3061 acgtaatgac atcctggctg tggctgactg gggacagaaa gtttccttct accagctgag
     3121 tggaaaacag attggaaagg atcgggcact gaactttgac ccctgctgca tcagctactt
     3181 tactaaaggc gagtacattt tgctgggggg ttcagacaag caagtatctc ttttcaccaa
     3241 ggatggagtg cggcttggga ctgttgggga gcagaactcc tgggtgtgga cgtgtcaagc
     3301 gaaaccggat tccaactatg tggtggtcgg ctgccaggac ggcaccattt ccttctacca
     3361 gcttattttc agcacagtcc atgggcttta caaggaccgc tatgcctaca gggatagcat
     3421 gactgacgtc attgtgcagc acctgatcac tgagcagaaa gttcggatta aatgcaaaga
     3481 gcttgtcaag aagattgcca tctacagaaa tcgattggct atccaactgc ccgagaaaat
     3541 cctcatctat gagttgtatt cagaggactt atcagacatg cattaccggg taaaggagaa
     3601 gattatcaag aagtttgagt gcaacctcct ggtggtgtgt gccaatcaca tcatcctgtg
     3661 ccaggagaaa cggctgcagt gcctgtcctt cagcggagtg aaggagcggg agtggcagat
     3721 ggagtctctc attcgttaca tcaaggtgat cggtggccct cctggaagag aaggcctctt
     3781 agtggggctg aagaatggac agatcctgaa gatcttcgtg gacaatctct ttgctatcgt
     3841 cctgctgaag caggccacag ctgtgcgctg cttggacatg agtgcctccc gtaagaagct
     3901 ggccgtggta gatgaaaatg acacttgcct ggtgtatgac atcgacacca aggagctgct
     3961 ttttcaggaa ccaaacgcca acagtgtagc ttggaacacc cagtgtgagg acatgctctg
     4021 cttctcggga ggaggctacc tcaacatcaa agccagcacc ttccctgtgc accggcagaa
     4081 gctgcagggc tttgtggtcg gctacaatgg ctccaagatc ttctgcctcc atgtcttctc
     4141 catttctgcc gtggaggtgc cgcagtccgc tcccatgtac cagtacctgg ataggaaact
     4201 gttcaaggaa gcctaccaga ttgcttgctt gggtgtcaca gacactgatt ggcgtgaact
     4261 ggccatggaa gcgctagaag gtttagattt tgaaactgca aagaaggcct tcatcagagt
     4321 acaagacctc cgatatttag agctcatcag cagcattgag gagaggaaga agcggggaga
     4381 gaccaacaat gacctgtttc tggcagatgt gttttcctac caggggaagt tccatgaggc
     4441 cgccaaactg tacaagagga gtgggcacga gaacctcgcg cttgaaatgt acaccgacct
     4501 ctgcatgttt gagtatgcca aggatttcct tggatctgga gaccccaaag aaacaaagat
     4561 gctaatcacc aaacaggctg actgggccag aaatatcaag gagcccaaag ccgccgtgga
     4621 gatgtacatc tcagcaggag agcacgtcaa ggccatcgag atctgtggtg accatggctg
     4681 ggttgacatg ttgatcgaca tcgcccgcaa actggacaag gctgagcgcg agcccctgct
     4741 gctgtgcgct acctacctca agaagctgga cagccctggc tatgctgctg agacctacct
     4801 gaagatgggt gacctcaagt ccctggtgca gctgcacgtg gagacccagc gctgggatga
     4861 ggcctttgct ttgggtgaga agcatcctga gtttaaggat gacatctaca tgccgtatgc
     4921 tcagtggcta gcagagaacg atcgctttga ggaagcccag aaagcgttcc acaaggctgg
     4981 gcgacagaga gaagcggtcc aggtgctgga gcagctcaca aacaatgccg tggcggagag
     5041 caggtttaat gatgctgcct attattactg gatgctatcc atgcagtgcc tcgatatagc
     5101 tcaagatcct gcccagaagg acacaatgct tggcaagttc taccacttcc agcgtttggc
     5161 agagctgtac catggttacc atgccatcca tcgccacacg gaagatccgt tcagtgtcca
     5221 tcgtcctgaa actcttttca acatctccag gttcctgctg cacagcctgc ccaaggacac
     5281 cccctcgggc atctctaaag tgaaaatact cttcaccttg gccaagcaga gcaaggccct
     5341 cggtgcctac aggctggccc ggcacgccta tgacaagctg cgtggcctgt acatccctgc
     5401 cagattccaa aagtccattg agctgggtac cctgaccatc cgcgccaagc ccttccacga
     5461 cagtgaggag ttggtgccct tgtgctaccg ctgctccacc aacaacccgc tgctcaacaa
     5521 cctgggcaac gtctgcatca actgccgcca gcccttcatc ttctccgcct cttcctacga
     5581 cgtgctacac ctggttgagt tctacctgga ggaagggatc actgatgaag aagccatctc
     5641 cctcatcgac ctggaggtgc tgagacccaa gcgggatgac agacagctag agattgcaaa
     5701 caacagctcc cagattctgc ggctagtgga gaccaaggac tccatcggag atgaggaccc
     5761 gttcacagct aagctgagct ttgagcaagg tggctcagag ttcgtgccag tggtggtgag
     5821 ccggctggtg ctgcgctcca tgagccgccg ggatgtcctc atcaagcgat ggcccccacc
     5881 cctgaggtgg caatacttcc gctcactgct gcctgacgcc tccattacca tgtgcccctc
     5941 ctgcttccag atgttccatt ctgaggacta tgagttgctg gtgcttcagc atggctgctg
     6001 cccctactgc cgcaggtgca aggatgaccc tggcccatga gtcgacggta ccgcgggccc
     6061 gggatccacc ggatctagat aactgatcga ctcctcaggt gcaggctgcc tatcagaagg
     6121 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgggatctt tttccctctg
     6181 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     6241 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     6301 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     6361 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     6421 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6481 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6541 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6601 tcttatgaag atccctcgac ttaattaagg tacccaattc gccctatagt gagtcgtatt
     6661 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6721 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6781 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6841 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6901 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6961 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7021 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7081 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7141 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7201 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7261 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7321 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7381 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7441 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7501 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7561 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7621 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7681 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7741 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7801 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7861 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7921 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7981 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8041 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8101 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8161 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8221 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8281 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8341 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8401 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8461 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8521 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8581 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8641 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8821 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9061 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9181 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9241 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9301 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9361 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9421 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9481 aaagctggag ctcatcgata c
//
