LOCUS       sequence-434280-        1107 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plasmid to express gRNA2 (aatttcgacgggccttcaag) for editing at the 
            end of Drosophila 5-HT1BR coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pdU6-2_sgRNA2-5-HT1BR
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..400
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
     misc_RNA        422..497
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(628..648)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(628..646)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(667..683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(667..683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(680..702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    691..707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(715..721,722..739,740..745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    760..781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(898..915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttcgacttg cagcctgaaa tacggcacga gtaggaaaag ccgagtcaaa tgccgaatgc
       61 agagtctcat tacagcacaa tcaactcaag aaaaactcga cactttttta ccatttgcac
      121 ttaaatcctt ttttattcgt tatgtatact ttttttggtc cctaaccaaa acaaaaccaa
      181 actctcttag tcgtgcctct atatttaaaa ctatcaattt attatagtca ataaatcgaa
      241 ctgtgttttc aacaaacgaa caataggaca ctttgattct aaaggaaatt ttgaaaatct
      301 taagcagagg gttcttaaga ccatttgcca attcttataa ttctcaactg ctctttcctg
      361 atgttgatca tttatatagg tatgttttcc tcaatacttc gaatttcgac gggccttcaa
      421 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      481 tggcaccgag tcggtgcgat ccattttttt gctcacctgt gattgctcct actcaaatac
      541 aaaaacatca aattttctgt caataaagca tatttattta tatttatttt acaggaaaga
      601 attactagtg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      661 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      721 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      781 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1081 ccgcccccct gacgagcatc acaaaaa
//
