LOCUS       sequence-434591-         904 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses mitochondrially targeted TALE-free DddAtox 1397N-UGI in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: TALE-free DddAtox 1397N
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             502..753
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg gatccggcag ctacgccctg
      181 ggtccgtatc agattagcgc cccgcagctg ccagcttaca atggtcagac cgtgggtacc
      241 ttctactatg tgaacgacgc gggcggtctg gagagcaagg tgtttagcag cggcggtcca
      301 accccgtacc caaactatgc caatgccggt catgtggagg gtcagagcgc cctgttcatg
      361 cgtgataacg gcatcagcga gggtctggtg ttccacaaca acccggaagg cacctgcggt
      421 ttttgcgtga acatgaccga gaccctgctg ccggaaaacg cgaaaatgac cgtggtgccg
      481 ccggaaggtt ctggcggctc aactaatctg agcgacatca ttgagaagga gactgggaaa
      541 cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc
      601 aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat
      661 gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat
      721 tctaacggcg agaataagat caagatgctg tgataagggg tctttgtcct ctgtactgtc
      781 tctctccttg cccctaaccc aaaaagcttc atttttctgt gtaggctgca caagagcctt
      841 gattgaagat atattctttc tgaacagtat ttaaggtttc caataaaatg tacacccctc
      901 agaa
//
