LOCUS       sequence-434713-        2929 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses mitochondrial cytosine base editor arm in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: TL1 alphaDdCBE 3_CN (right, DddA11-N)
FEATURES             Location/Qualifiers
     source          1..2929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2527..2778
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg acatcgcgga tctgcgtacc
      181 ctgggttaca gccagcagca gcaggagaag atcaagccaa aggtgcgcag caccgtggcc
      241 cagcaccatg aagctctggt gggtcacggc ttcacccacg cgcacatcgt ggctctgagc
      301 cagcacccag ccgcgctggg taccgtggcc gtgaaatatc aggacatgat tgctgccctg
      361 ccagaggcta cccatgaagc gattgtgggt gtgggcaaga gaggagccgg tgctcgtgcg
      421 ctggaggccc tgctgaccgt ggccggtgaa ctgcgtggcc cgccgctgca gctggatacc
      481 ggccagctgc tgaaaatcgc gaaacgtggc ggtgtgaccg ctgtggaagc tgtgcatgcc
      541 tggcgtaatg ctctgaccgg tgcccccctg aacctgaccc cggaccaagt ggtggctatc
      601 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      661 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cggtggcggc
      721 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
      781 accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg
      841 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      901 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
      961 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacatt
     1021 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
     1081 ggcctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc
     1141 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
     1201 gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg
     1261 ttgccggtgc tgtgccagga ccatggcctc acacccgatc aggtcgtggc tatcgccagc
     1321 aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag
     1381 gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa
     1441 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg
     1501 gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc aagcgctcga aacggtgcag
     1561 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
     1621 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
     1681 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc
     1741 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
     1801 accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg
     1861 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
     1921 gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
     1981 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagccacgat
     2041 ggcggcaagc aagcgctcga aagcattgtg gcccagctga gccggcctga tccggcgttg
     2101 gccgcgttga ccaacgatca cctggtggcc ctggcctgcc tgggcggtcg tccggccctg
     2161 gatgcggtga agaaaggcct gggtggatcc ggcagctacg ccctgggtcc gtatcagatt
     2221 agcgccccgc agctgccagc ttacaatggt cagaccgtgg gtaccttcta ctatgtgaac
     2281 gacgcgggcg gtctggagag caaggtgttt atcagcggcg gtccaacccc gtacccaaac
     2341 tatgtcagtg ccggtcatgt ggagggtcag agcgccctgt tcatgcgtga taacggcatc
     2401 agcgagggtc tggtgttcca caacaacccg aaaggcacct gcggtttttg cgtgaacatg
     2461 atcgagaccc tgctgccgga aaacgcgaaa atgaccgtgg tgccgccgga aggttctggc
     2521 ggctcaacta atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag
     2581 gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct
     2641 gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc
     2701 tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat
     2761 aagatcaaga tgctgtgata aggggtcttt gtcctctgta ctgtctctct ccttgcccct
     2821 aacccaaaaa gcttcatttt tctgtgtagg ctgcacaaga gccttgattg aagatatatt
     2881 ctttctgaac agtatttaag gtttccaata aaatgtacac ccctcagaa
//
