LOCUS       sequence-435613-        2433 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For creating CyanoTag vectors by Golden Gate cloning (BsaI version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2433)
  TITLE     CyanoTag plasmids, for native C-terminal fluorescent tagging of 
            proteins in cyanobacteria (S. elongatus 7942)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLM434
FEATURES             Location/Qualifiers
     source          1..2433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(64..652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(144..163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(823..1614,1615..1683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1446..1465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1684..1788)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2128..2433
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2312..2331
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtggtc tcacttgaga
       61 ctctttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      121 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      181 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      241 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      301 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      361 taactatcgt cttgagccca acccggtaag acacgactta tcgccactgg cagcagccac
      421 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      481 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      541 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      601 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      661 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      721 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      781 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct ggctccccgt
      901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      961 gcgtgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgga gtcagacttg
     1801 agacctaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     1861 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     1921 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     1981 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     2041 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     2101 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     2161 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     2221 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac
     2281 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     2341 ccggtgtccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     2401 aacgccatta acctgatgtt ctggggaata taa
//
