LOCUS       sequence-436977-        8112 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz 
            AA, Muenke M
  TITLE     A previously unidentified amino-terminal domain regulates 
            transcriptional activity of wild-type and disease-associated human 
            GLI2.
  JOURNAL   Hum Mol Genet. 2005 Aug 1. 14(15):2181-8.
  PUBMED    15994174
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2005 Aug 1. 14(15):2181-8."
COMMENT     Sequence Label: pCS2-MT GLI2 delta N
FEATURES             Location/Qualifiers
     source          1..8112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..1008
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1043..1061
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1043..1060
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1111..1140
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1150..1179
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1189..1218
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1228..1257
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1267..1296
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1330..1359
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    5172..5306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5196..5215)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5250..5269
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5419..5439)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5419..5437)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5458..5474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5458..5474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5471..5493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5482..5498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5506..5512,5513..5530,5531..5536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5551..5572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5689..5706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5860..6448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5940..5959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6619..7410,7411..7479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7242..7261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7480..7584)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7610..8065)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7747..7768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7959..7978
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgggcctctt cgctattacg ccagtcgacc gccaattcaa tatggcgtat atggactcat
       61 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
      121 gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat
      181 gccaattcaa tatggcggac ctggcactgt gccaactggg gaggggtcta cttggcacgg
      241 tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg
      301 tgccaataat ggcggccata ttggctatat gccaggatca atatataggc aatatccaat
      361 atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca
      421 tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc
      481 ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa
      541 tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg
      601 gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac
      661 gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt
      721 tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat
      781 ctattaatag taacttggca agtacattac tattggaagt acgccagggt acattggcag
      841 tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat
      901 tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt
      961 aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaaccg ccattctgcc
     1021 tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt
     1081 tttgcaggat cccatcgatt taaagctatg gagcaaaagc tcatttctga agaggacttg
     1141 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc
     1201 atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat
     1261 gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagag cttgggcgac
     1321 ctcaccatgg agcaaaagct catttctgaa gaggacttga attcaatggc cctcacctcc
     1381 atcaatgcca cgcccaccca gctcagcagc agcagcaact gtctgagtga caccaaccag
     1441 aacaagcaga gcagtgagtc ggccgtcagc agcaccgtca accctgtcgc cattcacaag
     1501 cgcagcaagg tcaagaccga gcctgagggc ctgcggccgg cctcccctct ggcgctgacg
     1561 cagggccagg tgtctggaca cggctcatgt gggtgtgccc ttcccctctc ccaggagcag
     1621 ctggctgacc tcaaggaaga tctggacagg gatgactgta agcaggaggc tgaggtggtc
     1681 atctatgaga ccaactgcca ctgggaagac tgcaccaagg agtacgacac ccaggagcag
     1741 ctggtgcatc acatcaacaa cgagcacatc cacggggaga agaaggagtt tgtgtgccgc
     1801 tggcaggcct gcacgcggga gcagaagccc ttcaaggcgc agtacatgct ggtggtgcac
     1861 atgcggcgac acacgggcga gaagccccac aagtgcacgt tcgagggctg ctcgaaggcc
     1921 tactcccgcc tggagaacct gaagacacac ctgcggtccc acaccgggga gaagccatat
     1981 gtgtgtgagc acgagggctg caacaaagcc ttctccaacg cctcggaccg cgccaagcac
     2041 cagaatcgca cccactccaa cgagaaaccc tacatctgca agatcccagg ctgcaccaag
     2101 agatacacag accccagctc tctccggaag catgtgaaaa cggtccacgg cccagatgcc
     2161 cacgtcacca agaagcagcg caatgacgtg cacctccgca caccgctgct caaagagaat
     2221 ggggacagtg aggccggcac ggagcctggc ggcccagaga gcaccgaggc cagcagcacc
     2281 agccaggccg tggaggactg cctgcacgtc agagccatca agaccgagag ctccgggctg
     2341 tgtcagtcca gccccggggc ccagtcgtcc tgcagcagcg agccctctcc tctgggcagt
     2401 gcccccaaca atgacagtgg cgtggagatg ccggggacgg ggcccgggag cctgggagac
     2461 ctgacggcac tggatgacac acccccaggg gccgacacct cagccctggc tgccccctcc
     2521 gctggtggcc tccagctgcg caaacacatg accaccatgc accggttcga gcagctcaag
     2581 aaggagaagc tcaagtcact caaggattcc tgctcatggg ccgggccgac tccacacacg
     2641 cggaacacca agctgcctcc cctcccggga agtggctcca tcctggaaaa cttcagtggc
     2701 agtgggggcg gcgggcccgc ggggctgctg ccgaacccgc ggctgtcgga gctgtccgcg
     2761 agcgaggtga ccatgctgag ccagctgcag gagcgccgcg acagctccac cagcacggtc
     2821 agctcggcct acaccgtgag ccgccgctcc tccggcatct ccccctactt ctccagccgc
     2881 cgctccagcg aggcctcgcc cctgggcgcc ggccgcccgc acaacgcgag ctccgctgac
     2941 tcctacgacc ccatctccac ggacgcgtcg cggcgctcga gcgaggccag ccagtgcagc
     3001 ggcggctccg ggctgctcaa cctcacgccg gcgcagcagt acagcctgcg ggccaagtac
     3061 gcggcagcca ctggcggccc cccgcccact ccgctgccgg gcctggagcg catgagcctg
     3121 cggaccaggc tggcgctgct ggacgcgccc gagcgcacgc tgcccgccgg ctgcccacgc
     3181 ccactggggc cgcggcgtgg cagcgacggg ccgacctatg gccacggcca cgcgggggct
     3241 gcgcccgcct tcccccacga ggctccaggc ggcggagcca ggcgggccag cgaccctgtg
     3301 cggcggcccg atgccctgtc cctgccgcgg gtgcagcgct tccacagcac ccacaacgtg
     3361 aaccccggcc cgctgccgcc ctgtgccgac aggcgaggcc tccgcctgca gagccacccg
     3421 agcaccgacg gcggcctggc ccgcggcgcc tactcgcccc ggccgcctag catcagcgag
     3481 aacgtggcga tggaggccgt ggcggcagga gtggacggcg cggggcccga ggccgacctg
     3541 gggctgccgg aggacgacct ggtgcttcca gacgacgtgg tgcagtacat caaggcgcac
     3601 gccagtggcg ctctggacga gggcaccggg caggtgtatc ccacggaaag cactggcttc
     3661 tctgacaacc ccagactacc cagcccgggg ctgcacggcc agcgcaggat ggtggctgcg
     3721 gactccaacg tgggcccctc cgcccctatg ctgggaggat gccagttagg ctttggggcg
     3781 ccctccagcc tgaacaaaaa taacatgcct gtgcagtgga atgaggtgag ctccggcacc
     3841 gtagacgccc tggccagcca ggtgaagcct ccaccctttc ctcagggcaa cctggcggtg
     3901 gtgcagcaga agcctgcctt tggccagtac ccgggctaca gtccgcaagg cctacaggct
     3961 agccctgggg gcctggacag cacgcagcca cacctgcagc cccgcagcgg agccccctcc
     4021 cagggcatcc ccagggtaaa ctacatgcag cagctgcgac agccagtggc aggcagccag
     4081 tgtcctggca tgactaccac tatgagcccc catgcctgct atggccaagt ccacccccag
     4141 ctgagcccca gcaccatcag tggggccctc aaccagttcc cccaatcctg cagcaacatg
     4201 ccagccaagc cagggcatct ggggcaccct cagcagacag aagtggcacc tgaccccacc
     4261 acgatgggca atcgccacag ggaacttggg gtccccgatt cagccctggc tggagtgcca
     4321 ccacctcacc cagtccagag ctacccacag cagagccatc acctggcagc ctccatgagc
     4381 caggagggct accaccaggt ccccagcctt ctgcctgccc gccagcctgg cttcatggag
     4441 ccccaaacag gcccgatggg ggtggctaca gcaggctttg gcctagtgca gccccggcct
     4501 cccctcgagc ccagccccac tggccgccac cgtggggtac gtgctgtgca gcagcagctg
     4561 gcctacgcca gggccacagg ccatgccatg gctgccatgc cgtccagtca ggaaacagca
     4621 gaggctgtgc ccaagggagc gatgggcaac atggggtcgg tgcctcccca gccgcctccg
     4681 caggacgcag gtggggcccc ggaccacagc atgctctact actacggcca gatccacatg
     4741 tacgaacagg atggaggcct ggagaacctc gggagctgcc aggtcatgcg gtcccagcca
     4801 ccacagccac aggcctgtca ggacagcatc cagccccagc ccttgccctc accaggggtc
     4861 aaccaggtgt ccagcactgt ggactcccag ctcctggagg ccccccagat tgacttcgat
     4921 gccatcatgg atgatggcga tcactcgagt ttgttctcgg gtgctctgag ccccagcctc
     4981 ctccacagcc tctcccagaa ctcctcccgc ctcaccaccc cccgaaactc cttgaccctg
     5041 ccctccatcc ccgcaggcat cagcaacatg gctgtcgggg acatgagctc catgctcacc
     5101 agcctcgccg aggagagcaa gttcctgaac atgatgacct agtctagaac tatagtgagt
     5161 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     5221 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5281 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5341 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     5401 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     5461 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5521 gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5581 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6301 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     7621 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     7681 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     7741 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7801 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     7861 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     7921 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     7981 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     8041 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     8101 gggcgatcgg tg
//
