LOCUS       sequence-437248-       10172 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: pLVX-NLS-HA-TurboID-PCNA
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2822..2831
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2840..2860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2873..2899
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2903..2929
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2933..2959
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2966..3922
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    4945..5511
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4961..5513
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5105..5122)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5332..5351
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5525..6127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRGDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6024..6044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6141..6729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6194..6214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6612..6623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6936..7569
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7697..7713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7697..7713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7710..7732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7721..7737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7745..7751,7752..7769,7770..7775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7790..7811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7928..7945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8099..8687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8179..8198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8858..9649,9650..9718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9719..9823)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9871..10005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9908..9927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9962..9981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 cgccaccatg ggagcatcac caaaaaagaa gagaaaggta ccggtggcta cctacccgta
     2881 tgatgttccg gattacgctg gctatcccta cgacgtgccc gactatgccg ggtaccccta
     2941 tgacgtccca gactacgcag ctagcaaaga caatactgtg cctctgaagc tgatcgctct
     3001 cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag
     3061 ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt
     3121 gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct
     3181 gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcaa ccaatcagta
     3241 cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca
     3301 ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct
     3361 gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg
     3421 aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc
     3481 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
     3541 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
     3601 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
     3661 caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga
     3721 gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag
     3781 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     3841 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     3901 cagtctgcgg tctgccgaaa agctcgaggg atccggtgga ggcgggtctg gaggcggggg
     3961 tagtggcggg ggtggaagcg ggggtggagg cgggtcgggt ggcggaggta gcggaggcgg
     4021 tggaagtgga ggcggtggaa gtggtggagg aggttctggt ggcggaggta gcggtggcgg
     4081 aggtagcgga ggcggtggaa gtggtggcgg aggtagcgga ggcggtggat cgaacgcgta
     4141 tcgatccatg ttcgaggcgc gcctggtcca gggctccatc ctcaagaagg tgttggaggc
     4201 actcaaggac ctcatcaacg aggcctgctg ggatattagc tccagcggtg taaacctgca
     4261 gagcatggac tcgtcccacg tctctttggt gcagctcacc ctgcggtctg agggcttcga
     4321 cacctaccgc tgcgaccgca acctggccat gggcgtgaac ctcaccagta tgtccaaaat
     4381 actaaaatgc gccggcaatg aagatatcat tacactaagg gccgaagata acgcggatac
     4441 cttggcgcta gtatttgaag caccaaacca ggagaaagtt tcagactatg aaatgaagtt
     4501 gatggattta gatgttgaac aacttggaat tccagaacag gagtacagct gtgtagtaaa
     4561 gatgccttct ggtgaatttg cacgtatatg ccgagatctc agccatattg gagatgctgt
     4621 tgtaatttcc tgtgcaaaag acggagtgaa attttctgca agtggagaac ttggaaatgg
     4681 aaacattaaa ttgtcacaga caagtaatgt cgataaagag gaggaagctg ttaccataga
     4741 gatgaatgaa ccagttcaac taacttttgc actgaggtac ctgaacttct ttacaaaagc
     4801 cactccactc tcttcaacgg tgacactcag tatgtctgca gatgtacccc ttgttgtaga
     4861 gtataaaatt gcggatatgg gacacttaaa atactacttg gctcccaaga tcgaggatga
     4921 agaaggatct taggcggccg cggatccccc ccccccccta acgttactgg ccgaagccgc
     4981 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5041 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5101 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5161 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5221 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     5281 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     5341 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5401 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     5461 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     5521 aaccatggtt accgagtaca agcccacggt gcgcctcgcc acccgcggcg acgtccccag
     5581 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5641 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5701 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5761 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5821 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5881 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5941 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6001 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6061 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6121 tgcctgaacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6721 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     6781 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6841 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6901 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     6961 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7021 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7081 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7141 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7201 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7261 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7321 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7381 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7561 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7621 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
     7681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7741 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7801 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7861 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9841 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
     9901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10021 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10081 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10141 atttgttaaa tatgtactac aaacttagta gt
//
