LOCUS       sequence-437300-        2753 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo-compatible Level 0 vector carrying terminator WRKY70.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Sarkisyan Lab
  TITLE     Self-sustained luminescent reporters for longitudinal salicylic and 
            jasmonic acids imaging in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: M6597
FEATURES             Location/Qualifiers
     source          1..2753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(843..947)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1015..1033
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1653..1670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1824..2412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1904..1923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2583..2753,1..621))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
       61 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
      121 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
      181 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
      241 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
      301 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
      361 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
      421 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
      481 atcgctgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
      541 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
      601 ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
      661 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
      721 ggatgcccga ggcatagact gtaccccaaa aaaaaacagt cataacaagc catgaaaacc
      781 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
      841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      961 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1021 cgtatcacga ggccctttcg tccactgaag agccacttcg tggtctcaag cgtatacttg
     1081 ttgaggaaaa acacatcgta ggaacaaaac acatcgtaaa ccctacttgg tgtatataca
     1141 tgtaattatg cgtatatgta tcatagggat taagtataaa ataaaataaa aattcatagc
     1201 gatcaagtat ttgttattga tgcagtagag gggggatgtc gacaaagaaa taataataga
     1261 gggaaatgtt gctactaaaa tagtagtggc taaaatcact atagtcagta gacgtcgtca
     1321 cgtcgactta cctctgcgca atacgacatc gtatttccaa gaaaaggatt gtgagttgga
     1381 aaaatcaaag tgttgagaat caatctaaag ggagggatgt tttaatccag caagagtttt
     1441 atttatagta ctatatgtac agtacataca ctcattagag aaaagggcaa aatatgtatt
     1501 atatgttttc cactctacat ggcctaatta atgattaaaa ctcatggtct tagtcctata
     1561 gtagtggtaa acaagctgag accacagagt gattaatgaa tcggccaacg cgcggggaga
     1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2581 agttatttgc cgactacctt ggtgatctcg ccttttacgt aatggacaaa ttcttccaac
     2641 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     2701 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agc
//
