LOCUS       sequence-437302-        9032 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo-compatible Level M vector encoding hygromycin resistance 
            cassette and a luciferase gene under control of ORCA3 promoter and 
            terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Sarkisyan Lab
  TITLE     Self-sustained luminescent reporters for longitudinal salicylic and 
            jasmonic acids imaging in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: M7138
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1026..1614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1515..1534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1950..3098
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3244..3268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3442..3621
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    3657..3712
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          4053..4242
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     terminator      4937..5644
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             6430..6441
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7809..7833
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7921..8631
                     /label=oriV
                     /note="incP origin of replication"
     CDS             join(8926..9032,1..685)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
       61 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      121 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      181 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      241 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      301 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      361 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      421 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
      481 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
      541 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
      601 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg tgaaaggcga
      661 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
      721 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
      781 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
      841 gggagatata tcatgctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1141 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1621 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
     1681 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
     1741 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
     1801 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
     1861 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
     1921 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
     1981 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
     2041 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
     2101 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
     2161 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
     2221 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
     2281 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
     2341 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
     2401 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
     2461 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
     2521 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
     2581 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
     2641 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
     2701 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
     2761 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
     2821 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
     2881 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
     2941 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
     3001 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
     3061 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
     3121 ctgggggttc agcagcccct gctcggatct gttggaccgg acagtagtca tggttgatgg
     3181 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3241 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3301 gcggacgttt ttaatgtact ggggttgaac actctgtggg tctcattacg aattcccatg
     3361 gggagagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     3421 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     3481 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     3541 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     3601 attcccctcg gtatccaatt agagtctcat attcactctc atactgtatt tttacaacaa
     3661 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt acaatgaaaa
     3721 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagtgtct
     3781 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     3841 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     3901 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     3961 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4021 tgcctgaaac cgaactgccc gctgttctgc aggtaaattt ctagtttttc tccttcattt
     4081 tcttggttag gacccttttc tctttttatt tttttgagct ttgatctttc tttaaactga
     4141 tctatttttt aattgattgg ttatggtgta aatattacat agctttaact gataatctga
     4201 ttactttatt tcgtgtgtct atgatgatga tgataactgc agccggtcgc ggaggccatg
     4261 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4321 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     4381 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     4441 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     4501 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     4561 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     4621 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     4681 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     4741 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     4801 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     4861 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     4921 aaggaatagg cttgtcctgc tttaatgaga tatgcgagaa gcctatgatc gcatgatatt
     4981 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     5041 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     5101 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     5161 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     5221 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     5281 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     5341 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     5401 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     5461 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     5521 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     5581 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     5641 gcaggaccgc tcagagaatt cgcatgcagc gtctagatta ccgaattaga tttatctata
     5701 cccctattga tttttcttaa tataatttta ttttttaaaa aatgaagaga ttgtaattgt
     5761 atttgggtct tcgaaactcg tgactgtcta gctgatcgaa gaccaattta atttaattaa
     5821 tgctttggta acctcacacc gtgggtagct tgctcatcta cttagttaaa aataataata
     5881 aaaaaattgt gtaattcatc atcaataaat tcataagcat taaaatgatt ttgtgaactt
     5941 ttttggatat aaaattttgt acattccaaa gatttaaaaa agaacatcga tgaagttaat
     6001 aattttacaa tgagggatta tcgggcttaa caaaaaccac gtaataatct ctcatttcac
     6061 agaatttttt taatcctcca cttttaataa ttaaaagcta gttaactacc tttggcattt
     6121 tcgacaatct taccagcagg atcgttagtc atgtagccaa tactagcttc gactatcatc
     6181 tcgacaactc tggcctcctc ttcgttcctt ggtgcgtaaa ggaaggtcca ctcagtagga
     6241 gggcctggga ctcctggtcc ggccagtggg tgacgctggc cccacccctt cttcaaaact
     6301 tccttaccat cctgaggagc aagagccaag tgcagagagc aatcatggta atcatgcatg
     6361 tgagcgatct cacgcttggt tcttcttacc gtatcgtgtg agggaatgtt aagaggatca
     6421 ttaataggga tagaggggag aaggaagatg gcctttgcat gaaactcaaa ttttgacgta
     6481 ctgagaaaag ttttgtctgt gtgtcttgct tgcacacgtg ggatcacctt atcgataagt
     6541 tctctggttg caaactcttg atctaccagc tgagtacgct gacgctgtat aatgtgggag
     6601 gttatggatg gccttggccc ttcacgaaac ggcaagtcgg caagccaacg cctcttttct
     6661 ggaagcctgt catagattgc caaccctttc tgctcttgcc taaaaagtga caagacacaa
     6721 acagtgatat agcctcggaa gttctgtggg gcatagctcg gaccgacttc aaggaacgtt
     6781 tgatagtctc ttcggataat tgggaatgct attgcacttg ccaagactac gccgcaggta
     6841 attgcgatgg atcgggatga caatttgctc agtctctcaa ggagactaga caaagagata
     6901 gtagacctca ttttttcttc ttttttagaa actgattttt tatgaagttt tttggatttg
     6961 gagtgatgaa tagagtgagg agtggaattt atatactaag aggtaaaaag gcacgtttga
     7021 aaaatggata aatattttat tgaaaaataa tttgtagagt gttccttgaa ggtccagcga
     7081 tggcgcttgg gaggtgcaat tagtcacgtt tacacagaat taaataatta tggaacacgt
     7141 atttcttaag taaattattt tgggccgata aattacgtaa aagaatataa aatttatatc
     7201 agaaaaaaat ttctgtctct atacatgttg tttttacttt ctttccaaaa aatgtggtaa
     7261 ttttttaaaa tttgaaaatt ttttgaagtt aaaaaaaaat aattttgaag attatataca
     7321 cgattcaatc aattttgaga taaaaatata aaagaaatat caaagattaa tataaataca
     7381 gtcatctaca catttgagcc accctctaac acgaataaaa gtattcaaca gacaatgatg
     7441 aaattacgca tgacgtgaga cagaaatatc cttactcacg taaaaaaata tgacttaaat
     7501 gtttagatag tgtattttaa aacaattata cattttggac ggaaagaaaa agttggcatt
     7561 gatgaatact gacgaagtcc ataccgtgat gatgataaag tatttttttg aaactagcca
     7621 ccacatttac ataataattg attcgttcat cttatccaac atttattttt tctgctccta
     7681 agcatttagt atagttttta ctctccgttt ttatttattt gggacatggt aagaattcct
     7741 cctgtgtgag accgaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta
     7801 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     7861 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc
     7921 cgcctttgcg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
     7981 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg
     8041 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
     8101 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
     8161 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
     8221 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
     8281 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
     8341 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
     8401 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
     8461 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     8521 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
     8581 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcca
     8641 attcccgagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     8701 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     8761 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     8821 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     8881 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     8941 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     9001 ccgacgttgc tggccgtaca tttgtacggc tc
//
