LOCUS       sequence-437303-       10343 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo-compatible Level M vector encoding hygromycin resistance 
            cassette and a luciferase gene under control of WRKY70 promoter and 
            terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Sarkisyan Lab
  TITLE     Self-sustained luminescent reporters for longitudinal salicylic and 
            jasmonic acids imaging in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: M7691
FEATURES             Location/Qualifiers
     source          1..10343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      711..1418
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             2281..2292
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4894..4918
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5006..5716
                     /label=oriV
                     /note="incP origin of replication"
     CDS             6011..6802
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7143..7731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7632..7651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8067..9215
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9361..9385
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        9559..9738
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    9774..9829
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          join(10170..10343,1..16)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 atgatgataa ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
       61 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
      121 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
      181 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
      241 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
      301 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
      361 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
      421 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
      481 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
      541 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
      601 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
      661 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taggcttgtc ctgctttaat
      721 gagatatgcg agaagcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
      781 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
      841 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
      901 gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt
      961 ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa
     1021 atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct
     1081 tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta
     1141 ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
     1201 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
     1261 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
     1321 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
     1381 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga ccgctcagag aattcgcatg
     1441 cagcgtatac ttgttgagga aaaacacatc gtaggaacaa aacacatcgt aaaccctact
     1501 tggtgtatat acatgtaatt atgcgtatat gtatcatagg gattaagtat aaaataaaat
     1561 aaaaattcat agcgatcaag tatttgttat tgatgcagta gaggggggat gtcgacaaag
     1621 aaataataat agagggaaat gttgctacta aaatagtagt ggctaaaatc actatagtca
     1681 gtagacgtcg tcacgtcgac ttacctctgc gcaatacgac atcgtatttc caagaaaagg
     1741 attgtgagtt ggaaaaatca aagtgttgag aatcaatcta aagggaggga tgttttaatc
     1801 cagcaagagt tttatttata gtactatatg tacagtacat acactcatta gagaaaaggg
     1861 caaaatatgt attatatgtt ttccactcta catggcctaa ttaatgatta aaactcatgg
     1921 tcttagtcct atagtagtgg taaacaagct agttaactac ctttggcatt ttcgacaatc
     1981 ttaccagcag gatcgttagt catgtagcca atactagctt cgactatcat ctcgacaact
     2041 ctggcctcct cttcgttcct tggtgcgtaa aggaaggtcc actcagtagg agggcctggg
     2101 actcctggtc cggccagtgg gtgacgctgg ccccacccct tcttcaaaac ttccttacca
     2161 tcctgaggag caagagccaa gtgcagagag caatcatggt aatcatgcat gtgagcgatc
     2221 tcacgcttgg ttcttcttac cgtatcgtgt gagggaatgt taagaggatc attaataggg
     2281 atagagggga gaaggaagat ggcctttgca tgaaactcaa attttgacgt actgagaaaa
     2341 gttttgtctg tgtgtcttgc ttgcacacgt gggatcacct tatcgataag ttctctggtt
     2401 gcaaactctt gatctaccag ctgagtacgc tgacgctgta taatgtggga ggttatggat
     2461 ggccttggcc cttcacgaaa cggcaagtcg gcaagccaac gcctcttttc tggaagcctg
     2521 tcatagattg ccaacccttt ctgctcttgc ctaaaaagtg acaagacaca aacagtgata
     2581 tagcctcgga agttctgtgg ggcatagctc ggaccgactt caaggaacgt ttgatagtct
     2641 cttcggataa ttgggaatgc tattgcactt gccaagacta cgccgcaggt aattgcgatg
     2701 gatcgggatg acaatttgct cagtctctca aggagactag acaaagagat agtagacctc
     2761 attttgttag ttttgaggaa gtttttggtg agtggtatct tatttttctt tcctcttggt
     2821 gtggtttgta tgattaaagg aaagaagaga agtgtgtgta aatataaggt ggaaataaga
     2881 tttgtaatga tgggcacttt ttattttgtt ggtttctttg attttttttt ttagaggaag
     2941 atgcgaggaa atgagaagga agaaagagag atgtgtgagg aaatgagatg gaacttgaac
     3001 ttatccaccc atttcttttg cttatggtga gcttgacgta agctcaaatg acgtagtgat
     3061 gaatgatgtc atttcatccc taagtgagaa tggaatatat ataattttgc tgaaatccaa
     3121 acttagggtc ctttaatgtt ttctataacc ggataaaatt tgtatcaaaa actagactaa
     3181 ttaaaagagt tatgaaaaac tatccattta aagattaaac aagattctaa atttcatttg
     3241 taaacttttt tgttcctcga gattgtacta ccaatttttt tttactgatg tagtaagatc
     3301 agttggaata gtgaattatt tatttgaaat ctcgagttaa atttgatatc caaatgatat
     3361 gatccgtttt tctatgaaga taattttgaa tagtagttta tatatcaaaa attcaaaaag
     3421 acatgtatat agtccttatc agctatcaat ccagatgtat accccctaaa tagacctact
     3481 atttcaaata gataacgaga cacgaaattt acaatcaact agtccgaaat gaattgccct
     3541 tgtaatcaaa tacttgaatg acagatataa ttgggagttg ggacggatta tctaaatcag
     3601 attgattacg ttgaaagaat caatatataa actaattaac tctttttttt ttgttaagtt
     3661 agactagtaa acgtatcacc tcgctgcact aatatataaa ctatatggag tatcaaagct
     3721 aaacttttct acccaatttt tttataggaa cctatcttat attatactta aatttaacat
     3781 attctataaa aatagcaaca caatctatcg ttgaaaagta ttttctttca cccggaaaca
     3841 ctttattccg ttttcgtgga aaaggtagag ttgggcgggg cattgattta aaactttaat
     3901 attagaagat tatttaaaga ggtgtatgtg gtcgatcgac tcaatggccc ctaggcccta
     3961 gctatctcaa tgcatacatg cgatgcggat gggttctcct cgcttagaga tgtctagttg
     4021 gtctatagtg ctccctcaca gcctcactct cttctatatg caccaagcca ccaaggtttt
     4081 tttaatgata catttatgcc caagtataaa ttaacattgg tgatattttt tttagatatg
     4141 acaagttttt gcatacccat tatcaaaaag tcggataata gtccataaat ttatcttacg
     4201 gaagcattat aagagaatgt taaattatta cattttttca cgtcgaactt ttaaattaat
     4261 gattttgaaa ttttttccct tcatacgaag tctattaacg ccagttgagc taggatcata
     4321 tcaatcaatt tcagaacaca taaacaattg catgggttaa gaatcgacaa agtattactt
     4381 ttttgtgtat aaagataaat tatgactaaa aagatactta atccgtttta aaatataaga
     4441 tgtttaacta aaaacacgta tattaaaaat tacatatttt taaaaagttc aaccaaacat
     4501 aaagaaaatg cataatataa ctaataacta atcataaaat attgcataag aacttgaaaa
     4561 tattacttta ttatgaaact atttgttttt ttttccaaaa catctcgtac tataaaatgg
     4621 aaagaatata gtttaggata agatttaagt tatttccggg atagtaccac ttaacttcag
     4681 accaaatttg tacgcaggtt cataaatgag atccgttatt aatgccgtaa agtttagtgg
     4741 ttgtgtctca actttttcat taattcaaaa aggttggctc tctagcctaa gttaatatgc
     4801 gagatactat gataaaccag ttgctcctgt gtgagaccga ggatgcacat gtgaccgagg
     4861 gacacgaagt gatccgttta aactatcagt gtttgacagg atatattggc gggtaaacct
     4921 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     4981 cgttcgtcca tttgtatgtg ccagccgcct ttgcgacgct caccgggctg gttgccctcg
     5041 ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt
     5101 gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg
     5161 acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag
     5221 atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg
     5281 aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg
     5341 ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga
     5401 cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt
     5461 ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac
     5521 ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg
     5581 ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg
     5641 cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc
     5701 accccaaaaa tggcagcgct ggccaattcc cgaggcacga acccagtgga cataagcctg
     5761 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     5821 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     5881 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     5941 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     6001 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     6061 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     6121 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     6181 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     6241 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     6301 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     6361 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     6421 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     6481 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     6541 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     6601 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     6661 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     6721 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtga aaggcgagat
     6781 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     6841 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     6901 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     6961 agatatatca tgctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7261 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7741 aacgcggcct ttttacggtt cctggcagat cctagatgtg gcgcaacgat gccggcgaca
     7801 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
     7861 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
     7921 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     7981 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     8041 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     8101 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     8161 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     8221 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     8281 ccgtggagcg ttcgcgtcgt cttgaacagg aggcggcagg tttggcgaag tcgatgacca
     8341 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     8401 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     8461 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     8521 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     8581 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     8641 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     8701 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     8761 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     8821 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     8881 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     8941 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     9001 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     9061 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     9121 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     9181 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     9241 ggggttcagc agcccctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct
     9301 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     9361 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     9421 gacgttttta atgtactggg gttgaacact ctgtgggtct cattacgaat tcccatgggg
     9481 agagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt
     9541 ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc gggtttctgg
     9601 agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa
     9661 ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt
     9721 cccctcggta tccaattaga gtctcatatt cactctcata ctgtattttt acaacaatta
     9781 ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca atgaaaaagc
     9841 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agtgtctccg
     9901 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     9961 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
    10021 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
    10081 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
    10141 ctgaaaccga actgcccgct gttctgcagg taaatttcta gtttttctcc ttcattttct
    10201 tggttaggac ccttttctct ttttattttt ttgagctttg atctttcttt aaactgatct
    10261 attttttaat tgattggtta tggtgtaaat attacatagc tttaactgat aatctgatta
    10321 ctttatttcg tgtgtctatg atg
//
