LOCUS       sequence-437466-       12052 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Transient expression of GFP-tagged CBPKTG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12052)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPKTG
FEATURES             Location/Qualifiers
     source          1..12052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5533..5542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7999..8718
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8840..8961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8877..8896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8931..8950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8968..9423)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9105..9126)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9317..9336
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9450..9554
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9556..9913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9556..9747
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9579..9599)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9764..9899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9948..10742
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9948..10742
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10002..10021)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10612..10631
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10930..10949)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10974..11021
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11350..11938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11839..11858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgg agcttctacg aggaggcagc
      901 ggctctagta tcaacccagg aataggaaat gtgagcgcca gcagccccgt gcagcagggc
      961 ctgggtggcc aggctcaagg gcagccgaac agtgctaaca tggccagcct cagtgccatg
     1021 ggcaagagcc ctctgagcca gggagattct tcagccccca gcctgcctaa acaggcagcc
     1081 agcacctctg ggcccacccc cgctgcctcc caagcactga atccgcaagc acaaaagcaa
     1141 gtggggctgg cgactagcag ccctgccacg tcacagactg gacctggtat ctgcatgaat
     1201 gctaacttta accagaccca cccaggcctc ctcaatagta actctggcca tagcttaatt
     1261 aatcaggctt cacaagggca ggcgcaagtc atgaatggat ctcttggggc tgctggcaga
     1321 ggaaggggag ctggaatgcc gtaccctact ccagccatgc agggcgcctc gagcagcgtg
     1381 ctggctgaga ccctaacgca ggtttccccg caaatgactg gtcacgcggg actgaacacc
     1441 gcacaggcag gaggcatggc caagatggga ataactggga acacaagtcc atttggacag
     1501 ccctttagtc aagctggagg gcagccaatg ggagccactg gagtgaaccc ccagttagcc
     1561 agcaaacaga gcatggtcaa cagtttgccc accttcccta cagatatcaa gaatacttca
     1621 gtcaccaacg tgccaaatat gtctcagatg caaacatcag tgggaattgt acccacacaa
     1681 gcaattgcaa caggccccac tgcagatcct gaaaaacgca aactgataca gcagcagctg
     1741 gttctactgc ttcatgctca taagtgtcag agacgagagc aagcaaacgg agaggttcgg
     1801 gcctgctcgc tcccgcattg tcgaaccatg aaaaacgttt tgaatcacat gacgcattgt
     1861 caggctggga aagcctgcca agttgcccat tgtgcatctt cacgacaaat catctctcat
     1921 tggaagaact gcacacgaca tgactgtcct gtttgcctcc ctttgaaaaa tgccagtgac
     1981 aagcgaaacc aacaaaccat cctggggtct ccagctagtg gaattcaaaa cacaattggt
     2041 tctgttggca cagggcaaca gaatgccact tctttaagta acccaaatcc catagacccc
     2101 agctccatgc agcgagccta tgctgctctc ggactcccct acatgaacca gccccagacg
     2161 cagctgcagc ctcaggttcc tggccagcaa ccagcacagc ctcaaaccca ccagcagatg
     2221 aggactctca accccctggg aaataatcca atgaacattc cagcaggagg aataacaaca
     2281 gatcagcagc ccccaaactt gatttcagaa tcagctcttc cgacttccct gggggccaca
     2341 aacccactga tgaacgatgg ctccaactct ggtaacattg gaaccctcag cactatacca
     2401 acagcagctc ctccttctag caccggtgta aggaaaggct ggcacgaaca tgtcactcag
     2461 gacctgcgga gccatctagt gcataaactc gtccaagcca tcttcccaac acctgatccc
     2521 gcagctctaa aggatcgccg catggaaaac ctggtagcct atgctaagaa agtggaaggg
     2581 gacatgtacg agtctgccaa cagcagggat gaatattatc acttattagc agagaaaatc
     2641 tacaagatac aaaaagaact agaagaaaaa cggaggtcgc gtttacataa acaaggcatc
     2701 ttggggaacc agccagcctt accagccccg ggggctcagc cccctgtgat tccacaggca
     2761 caacctgtga gacctccaaa tggacccctg tccctgccag tgaatcgcat gcaagtttct
     2821 caagggatga attcatttaa ccccatgtcc ttggggaacg tccagttgcc acaagcaccc
     2881 atgggacctc gtgcagcctc cccaatgaac cactctgtcc agatgaacag catgggctca
     2941 gtgccaggga tggccatttc tccttcccga atgcctcagc ctccgaacat gatgggtgca
     3001 cacaccaaca acatgatggc ccaggcgccc gctcagagcc agtttctgcc acagaaccag
     3061 ttcccgtcat ccagcggggc gatgagtgtg ggcatggggc agccgccagc ccaaacaggc
     3121 gtgtcacagg gacaggtgcc tggtgctgct cttcctaacc ctctcaacat gctggggcct
     3181 caggccagcc agctaccttg ccctccagtg acacagtcac cactgcaccc aacaccgcct
     3241 cctgcttcca cggctgctgg catgccatct ctccagcaca cgacaccacc tgggatgact
     3301 cctccccagc cagcagctcc cactcagcca tcaactcctg tgtcgtcttc cgggcagact
     3361 cccaccccga ctcctggctc agtgcccagt gctacccaaa cccagagcac ccctacagtc
     3421 caggcagcag cccaggccca ggtgaccccg cagcctcaaa ccccagttca gcccccgtct
     3481 gtggctaccc ctcagtcatc gcagcaacag ccgacgcctg tgcacgccca gcctcctggc
     3541 acaccgcttt cccaggcagc agccagcatt gataacagag tccctacccc ctcctcggtg
     3601 gccagcgcag aaaccaattc ccagcagcca ggacctgacg tacctgtgct ggaaatgaag
     3661 acggagaccc aagcagagga cactgagccc gatcctggtg aatccaaagg ggagcccagg
     3721 tctgagatga tggaggagga tttgcaagga gcttcccaag ttaaagaaga aacagacata
     3781 gcagagcaga aatcagaacc aatggaagtg gatgaaaaga aacctgaagt gaaagtagaa
     3841 gttaaagagg aagaagagag tagcagtaac ggcacagcct ctcagtcaac atctccttcg
     3901 cagccgcgca aaaaaatctt taaaccagag gagttacgcc aggccctcat gccaacccta
     3961 gaagcactgt atcgacagga cccagagtca ttacctttcc ggcagcctgt agatccccag
     4021 ctcctcggaa ttccagacta ttttgacatc gtaaagaatc ccatggacct ctccaccatc
     4081 aagcggaagc tggacacagg gcaataccaa gagccctggc agtacgtgga cgacgtctgg
     4141 ctcatgttca acaatgcctg gctctataat cgcaagacat cccgagtcta taagttttgc
     4201 agtaagcttg cagaggtctt tgagcaggaa attgaccctg tcatgcagtc ccttggatat
     4261 tgctgtggac gcaagtatga gttttcccca cagactttgt gctgctatgg gaagcagctg
     4321 tgtaccattc ctcgcgatgc tgcctactac agctatcaga ataggtatca tttctgtgag
     4381 aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc ttcacagccc
     4441 cagacgacaa tttcaaagga tcagtttgaa aagaagaaaa atgatacctt agaccccgaa
     4501 cctttcgttg attgcaagga gtgtggccgg aagatgcatc agatttgcgt tctgcactat
     4561 gacatcattt ggccttcagg ttttgtgtgc gacaactgct tgaagaaaac tggcagacct
     4621 cgaaaagaaa acaaattcag tgctaagagg ctgcagacca caagactggg aaaccacttg
     4681 gaagaccgag tgaacaaatt tttgcggcgc cagaatcacc ctgaagccgg ggaggttttt
     4741 gtccgagtgg tggccagctc agacaagacg gtggaggtca agcccgggat gaagtcacgg
     4801 tttgtggatt ctggggaaat gtctgaatct ttcccatatc gaaccaaagc tctgtttgct
     4861 tttgaggaaa ttgacggcgt ggatgtctgc ttttttggaa tgcacgtcca agaatacggc
     4921 tctgattgcc cccctccaaa cacgaggcgt gtgtacattt cttatctgga tagtattcat
     4981 ttcttccggc cacgttgcct ccgcacagcc gtttaccatg agatccttat tggatattta
     5041 gagtatgtga agaaattagg gtatgtgaca gggcacatct gggcctgtcc tccaagtgaa
     5101 ggagatgatt acatcttcca ttgccaccca cctgatcaaa aaatacccaa gccaaaacga
     5161 ctgcaggagt ggtacaaaaa gatgctggac aaggcgtttg cagagcggat catccatgac
     5221 tacaaggata ttttcaaaca agcaactgaa gacaggctca ccagtgccaa ggaactgccc
     5281 tattttgaag gtgatttctg gcccaatgtg ttagaagaga gcattaagga actagaacaa
     5341 gaagaagagg agaggggtgg cgaagagagc actgcagcca gtgaaaccac tgagggcagt
     5401 cagggcgaca gcgggaatgc cggcggtggg aacaacggcg gtaccaacgg gaacggtagc
     5461 agcatcagcc gcgccaacgg cggtgggccc agcatgccca acgtgtccaa tgacctgtcc
     5521 cagaagctgt atgccaccat ggagaagcac aaggaggtct tcttcgtgat ccacctgcac
     5581 gctgggcctg tcatcaacac cctgcccccc atcgtcgacc ccgaccccct gctcagctgt
     5641 gacctcatgg atgggcgcga cgccttcctc accctcgcca gagacaagca ctgggagttc
     5701 tcctccttgc gccgctccaa gtggtccacg ctctgcatgc tggtggagct gcacacccag
     5761 ggccaggacc gctttgtcta cacctgcaac gagtgcaagc accacgtgga gacgcgctgg
     5821 cactgcactg tgtgcgagga ctacgacctc tgcatcaact gctataacac gaagagccat
     5881 gcccataaga tggtgaagtg ggggctgggc ctggatgacg agggcagcag ccagggcgag
     5941 ccacagtcaa agagccccca ggagtcacgc cggctgagca tccagcgctg catccagtcg
     6001 ctggtgcacg cgtgccagtg ccgcaacgcc aactgctcgc tgccatcctg ccagaagatg
     6061 aagcgggtgg tgcagcacac caagggctgc aaacgcaaga ccaacggggg ctgcccggtg
     6121 tgcaagcagc tcatcgccct ctgctgctac cacgccaagc actgccaaga aaacaaatgc
     6181 cccgtgccct tctgcctcaa catcaaacac aagctccgcc agcagcagat ccagcaccgc
     6241 ctgcagcagg cccagctcat gcgccggcgg atggccacca tgaacacccg caacgtgcct
     6301 cagcagagtc tgccttctcc tacctcagca ccgcccggga cccccacaca gcagcccagc
     6361 acaccccaga cgccgcagcc ccctgcccag ccccaaccct cacccgtgag catgtcacca
     6421 gctggcttcc ccagcgtggc ccggactcag ccccccacca cggtgtccac agggaagcct
     6481 accagccagg tgccggcccc cccacccccg gcccagcccc ctcctgcagc ggtggaagcg
     6541 gctcggcaga tcgagcgtga ggcccagcag cagcagcacc tgtaccgggt gaacatcaac
     6601 aacagcatgc ccccaggacg cacgggcatg gggaccccgg ggagccagat ggcccccgtg
     6661 agcctgaatg tgccccgacc caaccaggtg agcgggcccg tcatgcccag catgcctccc
     6721 gggcagtggc agcaggcgcc ccttccccag cagcagccca tgccaggctt gcccaggcct
     6781 gtgatatcca tgcaggccca ggcggccgtg gctgggcccc ggatgcccag cgtgcagcca
     6841 cccaggagca tctcacccag cgctctgcaa gacctgctgc ggaccctgaa gtcgcccagc
     6901 tcccctcagc agcaacagca ggtgctgaac attctcaaat caaacccgca gctaatggca
     6961 gctttcatca aacagcgcac agccaagtac gtggccaatc agcccggcat gcagccccag
     7021 cctggcctcc agtcccagcc cggcatgcaa ccccagcctg gcatgcacca gcagcccagc
     7081 ctgcagaacc tgaatgccat gcaggctggc gtgccgcggc ccggtgtgcc tccacagcag
     7141 caggcgatgg gaggcctgaa cccccagggc caggccttga acatcatgaa cccaggacac
     7201 aaccccaaca tggcgagtat gaatccacag taccgagaaa tgttacggag gcagctgctg
     7261 cagcagcagc agcaacagca gcagcaacaa cagcagcaac agcagcagca gcaagggagt
     7321 gccggcatgg ctgggggcat ggcggggcac ggccagttcc agcagcctca aggacccgga
     7381 ggctacccac cggccatgca gcagcagcag cgcatgcagc agcatctccc cctccagggc
     7441 agctccatgg gccagatggc ggctcagatg ggacagcttg gccagatggg gcagccgggg
     7501 ctgggggcag acagcacccc caacatccag caagccctgc agcagcggat tctgcagcaa
     7561 cagcagatga agcagcagat tgggtcccca ggccagccga accccatgag cccccagcaa
     7621 cacatgctct caggacagcc acaggcctcg catctccctg gccagcagat cgccacgtcc
     7681 cttagtaacc aggtgcggtc tccagcccct gtccagtctc cacggcccca gtcccagcct
     7741 ccacattcca gcccgtcacc acggatacag ccccagcctt cgccacacca cgtctcaccc
     7801 cagactggtt ccccccaccc cggactcgca gtcaccatgg ccagctccat agatcaggga
     7861 cacttgggga accccgaaca gagtgcaatg ctcccccagc tgaacacccc cagcaggagt
     7921 gcgctgtcca gcgaactgtc cctggtcggg gacaccacgg gggacacgct agagaagttt
     7981 gtggagggct tgccggtcat ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc
     8041 atcctgatcg agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc
     8101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     8161 cctgtgccct ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc
     8221 taccccgatc acatgaagca gcacgacttc ttcaagagcg ccatgcctga gggctacatc
     8281 caggagcgca ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag
     8341 ttcgagggcg ataccctggt gaatcgcatc gagctgaccg gcaccgattt caaggaggat
     8401 ggcaacatcc tgggcaataa gatggagtac aactacaacg cccacaatgt gtacatcatg
     8461 accgacaagg ccaagaatgg catcaaggtg aacttcaaga tccgccacaa catcgaggat
     8521 ggcagcgtgc agctggccga ccactaccag cagaataccc ccatcggcga tggccctgtg
     8581 ctgctgcccg ataaccacta cctgtccacc cagagcgccc tgtccaagga ccccaacgag
     8641 aagcgcgatc acatgatcta cttcggcttc gtgaccgccg ccgccatcac ccacggcatg
     8701 gatgagctgt acaagtgagc ggccgcgact ctagatcata atcagccata ccacatttgt
     8761 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8821 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8941 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     9001 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     9061 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     9121 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     9181 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     9241 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     9301 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     9361 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     9421 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9481 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9541 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     9601 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9661 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9721 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9841 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9901 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     9961 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    10021 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    10081 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    10141 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    10201 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    10261 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    10321 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    10381 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    10441 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    10501 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    10561 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    10621 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    10681 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    10741 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    10801 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    10861 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    10921 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    10981 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    11041 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    11101 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    11161 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    11221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
