LOCUS       sequence-437711-       11145 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses of mcherry-tagged mTet1s in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 11145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pmcherry-mTet1s  pc3905
FEATURES             Location/Qualifiers
     source          1..11145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      866..1001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1060..1079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1114..1133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1328..1726)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1737..2309
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1897..1916)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2126..2143
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      6562..6571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6617..7324)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6892..6911)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7161..7179
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7321..7330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7365..7384)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7392..8408
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7446..7468)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8409..8667)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     enhancer        8669..8972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(9041..9059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9066..9083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9066..9082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9075..9097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9223..9678
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9310..9329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9520..9541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9745..9764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9864..9886
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9924..9942)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10010..10114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10115..10183,10184..10975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10333..10352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaatt agtcagcaac
      841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      961 tgagctattc cagaagtagt gaagaggctt ttttggaggc ctaggctttt gcaaaaagct
     1021 cctcgaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
     1081 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1141 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
     1201 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1261 atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct cctcggccac
     1321 gaagtgctta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     1381 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     1441 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     1501 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     1561 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     1621 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     1681 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcaagcttat
     1741 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     1801 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     1861 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     1921 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     1981 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     2041 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     2101 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     2161 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     2221 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     2281 gtaacgttag ggggggggag ggagaggggg ggggggggcg gatttcgtta actgcggccg
     2341 cttagaccca acgattgtag ggtcccgcaa catgagtgag agagtatggg gacacggtaa
     2401 caacattgtc tcgggttaag gttgatgcaa ctcgagaagg aatttggtgt gataaattgg
     2461 cttcggggga tacatcagga cactcccggt ctgcgggctt ttttttcgtt acttttttgc
     2521 atttacactt aattttgttg atatcaaaac catgattagg cttgtttagg cttttgtgct
     2581 ggtagaatac aagggacaca cgaaaaggga cccctcgttt gggggagcgc aaagaggtgg
     2641 tagcatgaag ctccttccga gcgcactcaa tgagcaccga gccgtgaatg ggtgctatcg
     2701 ccacgccacc aaaggaagga tcggcgtaga tctcctcact gtctgaccag aattcatcaa
     2761 gttgaggcaa gttatcgtcc tcggggtgct gaggctcatc cgcttcaggg tgccgctcat
     2821 cttccacctg acaggaagcc agtttctgag aattgctgag gagagggagc tgctggtttg
     2881 actggttaga agttagctgc tcagatggac tagtgagagg ctcagcatga acgggagaat
     2941 cggctgtggg aagagactgg tgaggatgag cagccacctc ggcaggctgc accgttccag
     3001 tacattcccc agcagctgca ttagcaccac tgtgtgctcc agaaggcatt gtgcagtgga
     3061 gaatactact tgtttctgca aacccacctg aggctgtttt actgtacgta caggtagatg
     3121 tttctgcgga ggaagcctgc aggggacaga agtctgtaga ttcgtctttc actaggtgag
     3181 aagtagatga ggctgatgaa aagctcttag tgttgtgtga acctggggct ccctttgggc
     3241 tggcagagat cctcttcctt ctgagaccag cagtacctga tttattgtgg ttctgcttca
     3301 ttttggccct cttcccacat cgaggaacag gctcagtaaa acgtagtcgc ctcttcctgg
     3361 ttggcccatt gacttggatg gccccagatt tgattttggc cttcatccct tccacggagc
     3421 caaattcatc agtgtctgca agccggtata gtggcaggac gtggagttgt tcatcctcgg
     3481 gacaatttgt gtcacggcca tctgctcgaa tcaacgtaca caccacggtg cttccgttgt
     3541 gcatgttgtg aatgtccttg tgagaatggg cacaaaaatc catgcaacag gtgacaccag
     3601 agaaaggacg cccctcttca tttccaagtc gacagtctcc agcaacttct tcatattcca
     3661 cctgattttg ataagcaact ggagccatct gcttgtaaag tggagctaat actgtagcca
     3721 attcttgtaa attcttttca agctgttttt cctctctgct tatgagggtc ttgcggtctg
     3781 gaatgatcca cccggttttc acgtcacttc cttcagaact cattccagta attctcttat
     3841 agtagttatg taaggggtag tttggagcaa gtctgaattt tctggggttt tcactcctcc
     3901 caaacttaca gccgttgaaa tacatgctcc acgaacagcc aaaggagaag gacgctccgc
     3961 aggtttttgg gtcgatgcct tgacaggtgc aggtacgctt tttgttgagg gtacatcttc
     4021 ggtctgtggg atgtccgctg taggacctca agttctcagt gagctctttg tacaggcggt
     4081 cagccatcag gcgagggata ccttcccaca gcaggataag gacaactatc acagccgtcg
     4141 aacagtgatg gtctacacgc tcacgaacca aacaaataag cttctcttca ggaccacttc
     4201 ttctgatcac ccacttggcg accgggcagc cctgagagct cttcccttcc ttccccgtga
     4261 acactatctt ctcaatccgg attgccttcc ccttctggcc aaacctagtc tccatgagct
     4321 ccctgacagc agccacactt ggtcctgccc caaggtgtgt ataatatggg cctttttctt
     4381 tttgtgtacc tccatcacag tcacagggtg cagcttcctg ttccttcaat ccagctatca
     4441 ggttttttgc aggcttgcta agtaagtcca tgatgctctc tttaaattct gactgtgcac
     4501 tgtctgtggt gggacccagt ggtccattgc ttgatgtttg agcttcacgc ttctccgtaa
     4561 caactgtaat tccatcgaaa tgtaacactt tccctttcct gaggattgat gctgggtctg
     4621 gcaagagtgt gtcaggggag gtaccaggca acgtcggtgg tggtatctga tgcaccaggt
     4681 tctcttgagc gcctagatga aactgtgttt gggcagcacc agccattata ttagcacact
     4741 ggttagatgg tggggactgg ggcagagggt gagcttcctg attgacactc accatgggct
     4801 gtccctgaga attatctgcc ggctctgcaa atgtctgccc accggattct gtcttgaatt
     4861 ggcatgttgg cagagaatct gatggttcaa ccttcacttt ctcctttgcc aattcagggt
     4921 ttcttgtgaa ttttatctga ttgataggag gaaataactc attatgttgt gagggtgttt
     4981 tactctgagt cacgggcttt aatatttggt taaagacagg aatatttctc aacagaacag
     5041 gaaaactacg tggactagac tggccaaacc tttgaaactt agatgacatc caaatgtcat
     5101 gcattttact atactctata gctagctgct cttgcttctt ctgatcattt tcttgagagc
     5161 ttcgggctgg gggtttcttt ttccgcttgt ttgcgtgtgg ggtcgctctt gcctttttct
     5221 gggccttctt tggctttgct gatggtacac tgggtttagt ttgtgtacta gagggtggct
     5281 tctgatgtac catgcgtttc tcctgactgt gtttttgctt tgtgttcttt gcaacaaggc
     5341 tttctggtgt actttcagca ttcctgacct cggcgttgat gtgattaatc gtggatgcaa
     5401 gttgggtcaa ttgtgctgcg acatcttctt caatttgact gtagggtgca tcctggcatg
     5461 atgggtcatg ggaactcaaa ttctgatcgc agcttagtac ctgactgcag ttttcaagat
     5521 tgtgactttc tatgggcaat ggatgtgaat tctcaaaacc atcaaaggga gctctcccac
     5581 cagcagcgcc tttcttctca gctgtaggtg aacttggata tccttgtgat tttataagtg
     5641 cttggttagt agcgaaacta tcaggtgact tgaatgttct gttctggcca ttaaagacaa
     5701 cagtgtcatg gtgaaggccc tttccttgaa cgtttgggtc ttggaggtct tttctcaccg
     5761 aatgaaggat agccttgcaa ctgttaagca gactagccgt tttctgatgc gcttcttcat
     5821 ccttctccgg cttggatggg gaggagctct ctgagggcgc ttctgagagc tgagtcaggg
     5881 cttctatggc taccacttgt tccagcgtta atctcctgtc cttcattagg gatagaagac
     5941 ttggctgaac cggagagcca tctttgggtt ctttgttggg taccttctca ggagggtgag
     6001 gtcgactccc agcgctgtgt gtattagggt gattattaaa gatggtgggt tctgcaccag
     6061 acctgcagtt caagccaggc ttctggaaga gcagggtgtt ccttggcccc ttgagatgat
     6121 cacactcccc cccggaggtg gtgacactca tggcatcctt gcagtttgag ggatcagtca
     6181 gcaatttaga ggaattattt cccaacgcct tgctaaattc ctcatgattc aatttgttga
     6241 gcggaaggtg tgtgtcagtg ggtaaacagt gtacattttt catgccgttc cttatggtct
     6301 gggcaaatgt cggagaaggg ttggtttgct gcttgtcatc ttcagagggc tgggggtttt
     6361 ccaccccagt taaattggca tcacagcttc ctttgacttg accctctgct aagtgtgatt
     6421 tcttgtttgg cgcccacttc tccacctcaa ttcccgtcat gccgcctgca ttcttcctta
     6481 cattttcaag gggatgtgtg atcaaatcca acctttgctc ttcttcccca tgaccacgtc
     6541 tactgcagtc catgcgatcg cccatggtgg cgtccacggc gctgttgctc gcctgggctc
     6601 tgctcctcag gccgctcttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
     6661 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
     6721 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
     6781 cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
     6841 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
     6901 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
     6961 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
     7021 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg
     7081 cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg
     7141 cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
     7201 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
     7261 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
     7321 ccatggtggc gggatccgaa ttctttgcca aaatgatgag acagcacaat aaccagcacg
     7381 ttgcccagga gctgtaggaa aaagaagaag gcatgaacat ggttagcaga ggctctagag
     7441 ccgccggtca cacgccagaa gccgaacccc gccctgcccc gtcccccccg aaggcagccg
     7501 tccccccgcg gacagccccg aggctggaga gggagaaggg gacggcggcg cggcgacgca
     7561 cgaaggccct ccccgcccat ttccttcctg ccggcgccgc accgcttcgc cccgcgcccg
     7621 ctagaggggg tgcggcggcg cctcccagat ttcggctccg cacagatttg ggacaaagga
     7681 agtccctgcg ccctctcgca cgattaccat aaaaggcaat ggctgcggct cgccgcgcct
     7741 cgacagccgc cggcgctccg ggggccgccg cgcccctccc ccgagccctc cccggcccga
     7801 ggcggccccg ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc
     7861 cccgcgccac gccccgtacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg
     7921 ggaggggggt gcaggggggg ttgcagcccg accgacgcgc ccacaccccc tgctcacccc
     7981 cccacgcaca caccccgcac gcagcctttg ttcccctcgc agcccccccc gcaccgcggg
     8041 gcaccgcccc cggccgcgct cccctcgcgc acactgcgga gcgcacaaag ccccgcgccg
     8101 cgcccgcagc gctcacagcc gccgggcagc gcggagccgc acgcggcgct ccccacgcac
     8161 acacacacgc acgcaccccc cgagccgctc cccccgcaca aagggccctc ccggagcccc
     8221 tcaaggcttt cacgcagcca cagaaaagaa acaagccgtc attaaaccaa gcgctaatta
     8281 cagcccggag gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag
     8341 agccggggcg ggcggcgcga ggcggcggcg gagcggggca cggggcgaag gcagcgcgca
     8401 gcgactcccg cccgccgcgc gcttcgcttt ttatagggcc gccgccgccg ccgcctcgcc
     8461 ataaaaggaa actttcggag cgcgccgctc tgattggctg ccgccgcacc tctccgcctc
     8521 gccccgcccc gcccctcgcc ccgccccccc cccccccccc cgcccccatc gctgcacaaa
     8581 ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag
     8641 tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt
     8701 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     8761 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     8821 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     8881 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     8941 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     9001 atgaccccgt aattgattac tattaataac taggacgcgt cctatagtga gtcgtattac
     9061 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     9241 cattgagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9301 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9361 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9421 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9481 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9541 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9601 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9661 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     9721 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     9781 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     9841 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     9901 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9961 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
