LOCUS       sequence-438016-        3502 bp    DNA     circular SYN 15-APR-2025
DEFINITION  meffBlue chromoprotein in a MultiGreen 1.0 HG entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Pennetti VJ, LaFayette PR, Parrott WA
  TITLE     MultiGreen: A multiplexing architecture for GreenGate cloning.
  JOURNAL   PLoS One. 2024 Sep 18;19(9):e0306008. doi: 
            10.1371/journal.pone.0306008. eCollection 2024.
  PUBMED    39292669
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-09-18"; volume: "19(9):e0306008. doi"; pages: " 
            10.1371/journal.pone.0306008. eCollection 2024"
COMMENT     Sequence Label: pVP084
FEATURES             Location/Qualifiers
     source          1..3502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..37
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        38..72
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     misc_feature    789..809
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(841..860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(842..860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(870..892)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1086..1105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1205..1227
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1265..1283)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1351..1455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1456..1524,1525..2316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1674..1693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2487..3075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2976..2995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3229..3246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3363..3384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3399..3404,3405..3422,3423..3429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3437..3453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3467..3485
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3467..3484
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taggacctag agacggaatt cgcggccgct tctagagttt acggctagct cagtcctagg
       61 cacaatgcta gctactagag gctctttaac aatttatcat gactaatagg aggtaacaga
      121 tgtccgttat cgcaacccag atgacgtaca aagtttatat gtcgggcacc gtgaatggtc
      181 attattttga agtcgaaggt gatggcaaag gtcgtccgta tgaaggcgaa cagaccgtca
      241 aactgaccgt gacgaaaggc ggtccgctgc cgtttgcatg ggatattctg agtccgcagt
      301 gccaatacgg ttccattccg ttcaccaaat atccggaaga tatcccggac tacgtcaaac
      361 agagctttcc ggaaggtttc acgtgggaac gcattatgaa ctttgaagat ggcgctgtgt
      421 gcaccgtttc aaacgacagc tctatccaag gcaactgctt cacgtatcat gtgaaatttt
      481 cgggtctgaa cttcccgccg aatggcccgg ttatgcagaa gaaaacccaa ggttgggaac
      541 cgcacagtga acgtctgttt gcgcgcggcg gtatgctgat cggcaacaat ttcatggccc
      601 tgaaactgga aggcggtggc cattatctgt gtgaatttaa aaccacgtac aaagcgaaaa
      661 aaccagtgaa aatgccgggt tatcattacg ttgatcgtaa actggacgtc acgaaccaca
      721 ataaagacta tacctcagtt gaacagtgtg aaatcagcat cgcacgcaag ccggtggtcg
      781 cctaataata ctagtagcgg ccgctgcagc gtctctgtat gtattgagac cgaattctcg
      841 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct
      901 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      961 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     1021 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     1081 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     1141 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1201 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1261 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1321 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1681 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1981 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2161 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3241 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3301 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3361 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     3421 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact
     3481 atagaagtga agcttggtct ca
//
