LOCUS       sequence-438036-        9860 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAV expression of hM4Di from RAM promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  TITLE     Nagai Lab plasmids for expression in astrocytes and neurons
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAM-d2TTA-TRE-hM4Di-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   31..160
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        174..488
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    182..200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    289..307
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    325..343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    360..378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    396..414
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     461..485
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      499..508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1957..1966
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1963..2670
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2108..2126)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2376..2395
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2766..3354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2819..2839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3237..3248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3390..3407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3396..3507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3655..3703
                     /note="synthetic polyadenylation signal"
     misc_feature    3717..3808
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3757..3776
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             join(4174..4791,4792..4797,4798..4833,4834..4836,4837..4872,
                     4873..4875,4876..4911,4912..4920)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4933..4949)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4974..4993)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4975..4993)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5019..5153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5183..5323
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5183..5312
                     /label=AAV2 ITR
     polyA_signal    5460..5594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5623..5788
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5971..6426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6058..6077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6268..6289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6453..6557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6558..6626,6627..7418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6776..6795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7589..8177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8078..8097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  8480..8704
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8771..9304)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9493..9498,9499..9515,9516..9521))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 catttatcag ggttattgtc tcagagcatg cctgcaggca gctgcgcgct cgctcgctca
       61 ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg
      121 cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgcat ctcgagttta
      181 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
      241 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      301 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
      361 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      421 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
      481 agatcgccac cggtgtttgc caccatggcc aacttcacac ctgtcaatgg cagctcgggc
      541 aatcagtccg tgcgcctggt cacgtcatca tcccacaatc gctatgagac ggtggaaatg
      601 gtcttcattg ccacagtgac aggctccctg agcctggtga ctgtcgtggg caacatcctg
      661 gtgatgctgt ccatcaaggt caacaggcag ctgcagacag tcaacaacta cttcctcttc
      721 agcctggcgt gtgctgatct catcataggc gccttctcca tgaacctcta caccgtgtac
      781 atcatcaagg gctactggcc cctgggcgcc gtggtctgcg acctgtggct ggccctggac
      841 tgcgtggtga gcaacgcctc cgtcatgaac cttctcatca tcagctttga ccgctacttc
      901 tgcgtcacca agcctctcac ctaccctgcc cggcgcacca ccaagatggc aggcctcatg
      961 attgctgctg cctgggtact gtccttcgtg ctctgggcgc ctgccatctt gttctggcag
     1021 tttgtggtgg gtaagcggac ggtgcccgac aaccagtgct tcatccagtt cctgtccaac
     1081 ccagcagtga cctttggcac agccattgct ggcttctacc tgcctgtggt catcatgacg
     1141 gtgctgtaca tccacatctc cctggccagt cgcagccgag tccacaagca ccggcccgag
     1201 ggcccgaagg agaagaaagc caagacgctg gccttcctca agagcccact aatgaagcag
     1261 agcgtcaaga agcccccgcc cggggaggcc gcccgggagg agctgcgcaa tggcaagctg
     1321 gaggaggccc ccccgccagc gctgccaccg ccaccgcgcc ccgtggctga taaggacact
     1381 tccaatgagt ccagctcagg cagtgccacc cagaacacca aggaacgccc agccacagag
     1441 ctgtccacca cagaggccac cacgcccgcc atgcccgccc ctcccctgca gccgcgggcc
     1501 ctcaacccag cctccagatg gtccaagatc cagattgtga cgaagcagac aggcaatgag
     1561 tgtgtgacag ccattgagat tgtgcctgcc acgccggctg gcatgcgccc tgcggccaac
     1621 gtggcccgca agttcgccag catcgctcgc aaccaggtgc gcaagaagcg gcagatggcg
     1681 gcccgggagc gcaaagtgac acgaacgatc tttgccattc tgctggcctt catcctcacc
     1741 tggacgccct acaacgtcat ggtcctggtg aacaccttct gccagagctg catccctgac
     1801 acggtgtggt ccattggcta ctggctctgc tacgtcaaca gcaccatcaa ccctgcctgc
     1861 tatgctctgt gcaacgccac ctttaaaaag accttccggc acctgctgct gtgccagtat
     1921 cggaacatcg gcactgccag gcgggatcca ccggtcgcca ccatggtgag caagggcgag
     1981 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2041 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2101 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2161 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2221 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2281 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2341 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2401 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2461 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2521 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2581 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2641 tccaccggcg gcatggacga gctgtacaag tctagagtcg acccgggcgg ccgcttcgag
     2701 cagacatgaa aacgctagcg gcgcgccagc gatccaccgg atctagataa ctgatcctta
     2761 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3181 tgctcgcctg tgttgccacc tggattctgc gcgggacgcc cttctgctac gtcccttcgg
     3241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgatc
     3361 gataccgtcg actagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3421 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3481 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3541 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca gactagtacg
     3601 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
     3661 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     3721 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     3781 gcaagtgcag gtgccagaac atttctctgg cctaactggc cgacgcgtac cctagaagtt
     3841 tgttcgtgac tcagactaga agtttgttcg tgactcagac tagaagtttg ttcgtgactc
     3901 agactagaag tttgttcgtg actcagaact cattcataaa acgcttgtta taaaagcagt
     3961 ggctgcggcg cctcgtactc caaccgcatc tgcagcgagc aactgagaag ccaagactga
     4021 gccggcggcc ggatccagct catgagccat ggcttcccgc cggaggtgga ggagcaggat
     4081 gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc
     4141 tgtgcttctg ctaggatcaa tgtggaattc accatgtcta gactggacaa gagcaaagtc
     4201 ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa
     4261 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     4321 gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc
     4381 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     4441 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     4501 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     4561 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag
     4621 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     4681 ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc
     4741 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     4801 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     4861 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     4921 ctaagtaagc atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata
     4981 gtgagtcgta ttacgcgcgc agcggccgac catggcccaa cttgtttatt gcagcttata
     5041 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctccggac
     5161 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     5221 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     5281 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggaagctgt aagcttgtcg
     5341 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     5401 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5461 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5521 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5581 atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc
     5641 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     5701 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     5761 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa
     5821 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca
     5881 cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca
     5941 acgcttattt gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     6001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6361 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     6421 caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6481 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6541 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6601 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6661 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6721 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6781 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6841 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6901 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6961 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7021 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7081 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7141 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7201 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7261 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7321 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7381 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7441 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7501 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7561 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7981 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8221 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8281 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8341 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     8401 tttcacaccg cagaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg
     8461 gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac aaaatagatc
     8521 taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag
     8581 ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct
     8641 gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg taaagactat
     8701 attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
     8761 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     8821 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     8881 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
     8941 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     9001 gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     9061 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     9121 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     9181 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     9241 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     9301 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     9361 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     9421 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     9481 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     9541 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     9601 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
     9661 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga
     9721 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
     9781 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt
     9841 ggttggctac attattgaag
//
