LOCUS       sequence-438161-       12186 bp    DNA     circular SYN 15-APR-2025
DEFINITION  FAP-tagged marker for the trans-Golgi (FAP optimized for yeast 
            expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS413-TEF1pr-SEC7-FAPoptim
FEATURES             Location/Qualifiers
     source          1..12186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(201..220)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     320..342
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(380..398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    446..949
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        979..1083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1084..1152,1153..1944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1302..1321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2115..2703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2604..2623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2857..2874
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2991..3012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3027..3032,3033..3050,3051..3057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3065..3081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3070..3092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3089..3105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3089..3105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3124..3144
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3126..3144
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3162..3560
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     3564..3580
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3564..3580
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             10395..10424
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10425..10454
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(10489..10508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10490..10508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10518..10535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10518..10534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10527..10549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10675..11130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10762..10781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10972..10993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(11390..12052)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(join(12053..12186,1..54))
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 aaaaaaaaaa atgaaaatca ttaccgaggc ataaaaaaat atagagtgta ctagaggagg
       61 ccaagagtaa tagaaaaaga aaattgcggg aaaggactgt gttatgactt ccctgactaa
      121 tgccgtgttc aaacgatacc tggcagtgac tcctagcgct caccaagctc ttaaaacggg
      181 aatttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      241 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      301 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      361 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      421 ataatggttt cttagggtcc ttttcatcac gtgctataaa aataattata atttaaattt
      481 tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag
      541 tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta
      601 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag
      661 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
      721 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
      781 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
      841 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
      901 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcaggtggc
      961 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1021 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1081 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1141 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1201 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1261 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1321 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1381 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1441 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1501 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1561 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1621 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1681 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1741 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1801 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1861 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1921 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2761 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2821 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2881 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2941 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     3001 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3061 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     3121 cgcgcaatta accctcacta aagggaacaa aagctggagc tcatagcttc aaaatgtttc
     3181 tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta ccacttcaaa
     3241 acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt cgttaattac
     3301 ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa
     3361 aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt tgattttttt
     3421 ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat ttctcaagtt
     3481 tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa
     3541 gcatagcaat ctaatctaag ttttctagaa ctagtggatc catgtctgaa cagaattcag
     3601 tagtaaatgc tgaaaaaggg gatggcgaaa tatcttcaaa tgtagagact gcttcttcag
     3661 tcaatccgtc tgtgaaacca cagaatgcta ttaaagaaga agctaaagag acaaatggtg
     3721 aagatcaaaa atgcaaaggg ccagaaaacg ctggcagtac ggcagaaaca aaagaaacaa
     3781 gtaacgacgc taccaacgga atgaaaacgc ccgaagaaac ggaagataca aatgacaaac
     3841 gccatgacga tgaaggtgaa gatggagatg aagatgaaga tgaagatgaa gatgaagatg
     3901 aagacaatgg ggacgaggat gatgaggacg tggatagcag tagttccgaa accagttccg
     3961 aagacgggga agattctgaa tcagtctcag gggaaagtac tgagagtagc tctggagaag
     4021 atgaagaatc tgatgaaagc gatggaaata catcaaacag ctcctctggt gatgaaagcg
     4081 ggtcagaaga agaagaagaa gaagaagaag aagaagaaga agaggaaaat gctggcgaac
     4141 cagctatcgc tcatcaagat agtgttccca ccaacgattc tactgcccca aggtctaccc
     4201 atacgaggaa catatcacta tcttcaaatg gttcgaacac aaactcaacc ataattttag
     4261 tgaaaactac gttggagaca attttaaatg ataaggacat taaaaagaat tcgaatgctc
     4321 agaaggctat cgaaaggaca ttacaaaaat ttaaggaatt tgatccgcaa accacgaata
     4381 acccacatta cgtggattca atactagtat tcgaagcact aagggcaagc tgtcgtacca
     4441 aatcctccaa agttcaaagt ttggctttag attgcctgtc aaaattgttt tcttttagat
     4501 cgctagacga gaccctgtta gtgaatccac ccgattcttt agcctctaat gatcaacgac
     4561 aagatgctgc cgatggaata acgccgcctc caaaacaaaa aattatagac gctgcaattg
     4621 atactatttc agactgtttt caaggtgaag gcactgatga ccgtgtggaa ctacaaatcg
     4681 ttagagcttt atctagttgc attttagaag aggattcaag ttctttatgc cacggtgctt
     4741 ccttgctaaa ggctatcaga acaatctaca acgttttcgt cttttcgttg aacccatcca
     4801 atcaaggtat tgcacaggcg accttgacac aaattattag ttcggtgtat gataaaatcg
     4861 atctcaaaca aagtacctct tcagcagtat ccttatcgac aaaaaatcat caacaacaat
     4921 ccgccataga actttccgag gcttctgaaa atgcagaaac gccggctcct ttaactctgg
     4981 aaaatatgga taagttgaat gacgatgagg aaagactaat ggacgctcaa cagcctgatt
     5041 ctatcgccat aactaaccaa gatctagctg ttaaagatgc gttcttagtg tttagagtca
     5101 tggcgaaaat atgtgctaaa cctttggaaa cagaactcga catgaggtca catgccgtca
     5161 ggtcaaagct tttatctctt cacatcattt actctattat caaagatcat attgacgtat
     5221 tcctttccca caacattttt ctaccaggaa aggagcgtgt gtgctttatc gattcaatta
     5281 gacaatattt acgtcttgtt ttatcaagga atgctgcctc gcctctagct ccagttttcg
     5341 aggttacttt agaaattatg tggctattga ttgctaattt gagagcagat ttcgtgaagg
     5401 aaattccagt ttttttaaca gaaatctact tccccatttc agaattaacc acttccacct
     5461 cccaacaaaa gagatatttt ttaagtgtta ttcaacgaat ttgtaacgac ccaagaactt
     5521 tagttgaatt ttacttgaat tatgattgta accctggaat gccaaatgta atggaaataa
     5581 ctgttgatta tttgacaaga ttggctttaa ctagggtgga aattactcag actcaaagat
     5641 catactacga tgaacaaata tcaaaatccc tgtccaccta caatttctca caattgcccc
     5701 ttttgacttc ttccaacttg tcttctagtc ctgatgttgg tcaagtcaat ttactttttc
     5761 cacttgattt tgctctcaaa atggtgtcac taaattgcat tgtgtcagtt ttgcgttcct
     5821 taagctcatg ggctcacaaa gctttaaatc caaacacaca cactgctaat aaggtattac
     5881 taaacacaac atcttcagct cgtcaagaat ctagatcatc tttgagtaac gacgtaaggt
     5941 cttctattat gacaagtaat gatgacttca aaccaacata tgaagacgaa gaaagcagat
     6001 cattgagcag tcaaaacatt gacgcagacg accccacaca atttgaaaat ttgaaactaa
     6061 ggaaaactgc tttatcggaa tgtattgcta ttttcaacaa taaacccaag aaagctattc
     6121 cagtacttat taaaaaagga tttttgaaag atgattctcc aatttccatt gccaagtggt
     6181 tattagaaac agaaggcctc gacatggccg ctgttgggga ttatctaggc gaaggagacg
     6241 acaagaacat cgctataatg cacgcatttg ttgatgagtt tgacttcact ggtatgtcca
     6301 ttgttgacgc attaaggtca tttttacaaa gtttcagatt gcctggagaa ggtcaaaaaa
     6361 ttgacagatt catgctgaaa tttgcggaaa gatttgtgga ccaaaacccc ggagtctttt
     6421 caaaggcgga tactgcatat gtgctttcgt attctttgat catgttgaat actgatttac
     6481 attcgtcaca aatcaaaaat aaaatgtctt tacaagagtt tttagaaaac aacgaaggta
     6541 ttgacaacgg aagagattta ccaagagact tcttggaagg tttgttcaac gaaattgcta
     6601 acaatgaaat caagttaatt tctgaacagc atcaggcaat gctttcaggt gataccaatc
     6661 ttgtccaaca acagcaatct gctttcaact tctttaattc tcgtgatttg acaagagagg
     6721 catataatca agtctcaaaa gaaatttcat ctaaaacgga attagtcttt aagaatttaa
     6781 acaaaaataa aggaggccca gacgtctatt atgctgcttc ccacgttgag catgttaaat
     6841 caattttcga gacactatgg atgtcctttt tagcagctct aaccccccca tttaaggatt
     6901 atgatgacat tgacacaacc aataagtgtt tagaaggctt gaaaatatca attaaaattg
     6961 cttctacttt tagaatcaat gatgctagaa cctcctttgt aggtgcgtta gtccaatttt
     7021 gtaaccttca aaaccttgaa gaaatcaaag ttaaaaatgt caatgcaatg gtaattcttc
     7081 ttgaagtcgc gttatcagaa ggaaattact tggagggatc gtggaaggac attttgctgg
     7141 tcgtgtctca aatggaaaga ctacaattga tatccaaagg tatcgacaga gatacggttc
     7201 cagatgttgc acaagctcgt gttgcaaacc ctagagtttc ttacgaatca tcaagatcca
     7261 ataatacatc tttctttgat gtttggggca agaaggcaac tcccacagaa ttggctcaag
     7321 aaaaacacca taatcaaacc ttatcacccg aaatctctaa attcatttcc tccagtgaat
     7381 tagtcgtttt gatggacaat atatttacca aaagttccga gttatcaggt aatgctatcg
     7441 ttgattttat caaagcttta accgctgtat ctttagaaga aattgaatca tctgaaaatg
     7501 cttccacacc aagaatgttt tccttgcaaa aaatggtcga tgtatgttac tacaatatgg
     7561 atcgtatcaa actagaatgg acgccgcttt gggctgttat gggaaaagct ttcaacaaga
     7621 ttgctacaaa ctctaactta gcagtagtat ttttcgctat cgattccctg cgtcaattgt
     7681 ctatgagatt tttagatatt gaggaattat caggttttga atttcaacat gattttttaa
     7741 aaccttttga atacacggtt caaaatagtg gcaacactga agttcaagaa atgattattg
     7801 agtgttttag gaacttcata ttgacaaaat cggaaagtat caaatctggc tggaagccta
     7861 ttcttgaatc cttacaatat acggctcgct caagcactga atccattgta ttaaagacgc
     7921 aattgctggt tagcaatgat attgtgacaa atcacttcga aaacgtattc tctcaagaag
     7981 atgccttttc tgagttagtt ggtgtcttca gagaaatcac caaaaataag agattccaaa
     8041 agctatctct acatgctttg gagtctctaa gaaagatgac tcagaacgtc gcagacatct
     8101 gtttttacaa tgaaaataag actgaagaag aaagaaaaca taacgatgct ttacttcgtg
     8161 ggaaagacat attccaggat gtgtggttcc ctatgttatt ctgcttcaat gatacgatca
     8221 tgacagctga agacttagaa gttagatcac gtgcattaaa ctatatgttc gatgccctag
     8281 tggcatatgg tggtaaattc aatgatgatt tctgggaaaa gatttgtaag aagttactat
     8341 ttcctatttt cggtgtatta tccaaacatt gggaagtcaa ccaattcaat agtcatgatg
     8401 atttaagtgt ttggctttcg accacgttaa ttcaagcctt aaggaacttg attgcccttt
     8461 ttacgcatta ctttgaatcg ttgaacagaa tgctcgatgg atttttgggt ctgctggtat
     8521 cctgtatttg tcaagaaaat gacactattg ctagaattgg gagatcctgc ctgcaacaat
     8581 tgatattgca aaacgtatct aaattcaatg agtaccattg gaatcaaata ggtgacgtat
     8641 tcgataaatt gtttgattta accactgcta atgaattgtt cgattatgat ccgttacagc
     8701 aaggaagaaa atcatctgta tctcatcacc aaacaactaa tgataccagc caacactcag
     8761 atgatgatag caacgacaga agggagaacg attctaatat cagtgagacg gtcgaaagag
     8821 cgcatcaaga agaatccagc gaagacgttg gtggtgacat ggttgaaaca ctcaatgggc
     8881 aaactaagct taataatggc aattccgttc cgacggtaaa ggacgaattg aatccaaagc
     8941 ccgctagtct aagtatcccc aagaaaacta agcacatgaa acgaaacgaa agtaacgaag
     9001 atatacgtag gagaataaac atcaagaatt ctattgttgt caaatgtgtt ttacagcttt
     9061 tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac tgtatccctt
     9121 acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt tctcgtgact
     9181 ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt gataaaatac
     9241 caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt atgtttcagt
     9301 tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc accatttgca
     9361 tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt agtatcaatg
     9421 cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat gatgaagact
     9481 ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat aagagcgttc
     9541 caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa ttataccttt
     9601 ctactgatcc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacgcgggtg
     9661 cgggtgcggg tatgcaagct gttgttactc aagaaccatc tgttactgtt tctccaggtg
     9721 gtactgttat cttgacttgt ggttctggta ctggtgctgt tacttctggt cactacgcta
     9781 actggttcca acaaaagcca ggtcaagctc caagagcttt gatcttcgac actgacaaga
     9841 agtactcttg gactccaggt agattctctg gttctttgtt gggtgctaag gctgctttga
     9901 ctatctctga cgctcaacca gaagacgaag ctgaatacta ctgttctttg tctgacgttg
     9961 acggttactt gttcggtggt ggtactcaat tgactgtttt gtctggtggt ggtggttctg
    10021 gtggtggtgg ttctggtggt ggtggttctg gtggtggtgg ttctcaagct gttgttactc
    10081 aagaaccatc tgttactgtt tctccaggtg gtactgttat cttgacttgt ggttctggta
    10141 ctggtgctgt tacttctggt cactacgcta actggttcca acaaaagcca ggtcaagctc
    10201 caagagcttt gatcttcgac actgacaaga agtactcttg gactccaggt agattctctg
    10261 gttctttgtt gggtgctaag gctgctttga ctatctctga cgctcaacca gaagacgaag
    10321 ctgaatacta ctgttctttg tctgacgttg acggttactt gttcggtggt ggtactcaat
    10381 tgactgtttt gtctgagcag aagctgatca gcgaggagga cctggagcag aagctgatca
    10441 gcgaggagga cctgtaacgc ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
    10501 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
    10681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    10741 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10861 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10981 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    11041 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    11101 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
    11161 gtgcggtatt tcacaccgca tagatccgtc gagttcaaga gaaaaaaaaa gaaaaagcaa
    11221 aaagaaaaaa ggaaagcgcg cctcgttcag aatgacacgt atagaatgat gcattacctt
    11281 gtcatcttca gtatcatact gttcgtatac atacttactg acattcatag gtatacatat
    11341 atacacatgt atatatatcg tatgctgcag ctttaaataa tcggtgtcac tacataagaa
    11401 cacctttggt ggagggaaca tcgttggtac cattgggcga ggtggcttct cttatggcaa
    11461 ccgcaagagc cttgaacgca ctctcactac ggtgatgatc attcttgcct cgcagacaat
    11521 caacgtggag ggtaattctg ctagcctctg caaagctttc aagaaaatgc gggatcatct
    11581 cgcaagagag atctcctact ttctcccttt gcaaaccaag ttcgacaact gcgtacggcc
    11641 tgttcgaaag atctaccacc gctctggaaa gtgcctcatc caaaggcgca aatcctgatc
    11701 caaacctttt tactccacgc acggccccta gggcctcttt aaaagcttga ccgagagcaa
    11761 tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa gtcaccaatg cactcaacga
    11821 ttagcgacca gccggaatgc ttggccagag catgtatcat atggtccaga aaccctatac
    11881 ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct tctgcctctt
    11941 tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag atcgcaatct
    12001 gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc atctttgcct
    12061 tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca ttactttata
    12121 taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga gtcatccgct
    12181 aggtgg
//
