LOCUS       sequence-438446-       11304 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Assembled Tn10 with ptac promoter driving gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Alker AT, Farrell MV, Demko AM, Purdy TN, Adak S, Moore BS, Sneed 
            JM, Paul VJ, Shikuma NJ
  TITLE     Linking bacterial tetrabromopyrrole biosynthesis to coral 
            metamorphosis.
  JOURNAL   ISME Commun. 2023 Sep 19;3(1):98. doi: 10.1038/s43705-023-00309-6.
  PUBMED    37726481
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME 
            Commun."; date: "2023-09-19"; volume: "3(1):98. doi"; pages: " 
            10.1038/s43705-023-00309-6"
COMMENT     Sequence Label: pMMK805
FEATURES             Location/Qualifiers
     source          1..11304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1452..1471)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1463..2278
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1463..2278
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1536..1555)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(2324..2351)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2367..2438)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(2648..2695)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3528..3544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3552..3558,3559..3574,3575..3580))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    6341..6357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6365..6371,6372..6387,6388..6393))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        6615..6692
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6693..7775
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6712..6731)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        7882..7986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7987..8055,8056..8847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8205..8224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(8904..8923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9064..9086
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9225..9244
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            9925..10034
                     /note="incP origin of transfer"
     CDS             10067..10438
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      join(11056..11304,1..140)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
       61 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
      121 ggaacactta acggctgaca tgggaattcg gtatacatca ctttatttaa aacgatgccc
      181 attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg
      241 gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg
      301 ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa
      361 tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt
      421 ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt
      481 gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa
      541 ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta
      601 ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt
      661 ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa
      721 gctcagggga gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc
      781 cgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt
      841 tagcttcaaa taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt
      901 aaatagcaat aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg
      961 cgatatgcat aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttcc
     1021 tgacggaatg ttaattctcg ttgaccctga gcactgatga atcccctaat gattttggta
     1081 aaaatcatta agttaaggtg gatacacatc ttgtcatatg atcccggatc cggctgtaat
     1141 ccgggcagcg caacggaaca ttcatcagtg taaaaatgga atcaataaag ccctgcgcag
     1201 cgcgcagggt cagcctgaat acgcgtggcc gcctaggccg cggccgccgg gcaagtacga
     1261 catcacccgg cccaaggcgg caggctgact cacgttaagg gattttggtc atgactgatc
     1321 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg
     1381 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1441 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctccaggcc
     1501 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1561 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     1621 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1681 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1741 tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata
     1801 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1861 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca
     1921 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1981 gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt
     2041 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     2101 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     2161 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     2221 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     2281 agaattggtt aattggttgt aacacgcggc cgctcggtat aaacgcagaa aggcccaccc
     2341 gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga
     2401 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggtgta ctgcagtgca
     2461 ctagtggttc gtaacatctc tgtaactgct tgagacgtgg actgttgtga tatgccatat
     2521 atgcagtggt gttcgccggc gctggttccg gctgtcttgc ttagttgtga gtcgccaggc
     2581 agtcatcggt atgatctgta catgattcgt cagtgtgagc accactgacg agcagatttc
     2641 cagccagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttagg
     2701 atttatttgt atagttcatc catgccgtgt gtaatcccag cagccgtgac aaactcaaga
     2761 aggaccatgt ggtcgcgctt ttcgttggga tctttcgaaa gggcagattg agtggacagg
     2821 taatggttgt ctggtaacag cacaggccca tcgccaattg gggtgttttg ttggtaatgg
     2881 tcggctagtt gaacgcttcc atcctcaatg ttgtgtctga tcttgaagtt aaccttgata
     2941 ccgttctttt gcttgtcggc cataatgtac acattatggg agttatagtt atattccagt
     3001 ttgtggccta aaatatttcc atcttcctta aagtcaatac cctttaactc gattctatta
     3061 acaagggtat caccctcgaa cttcacttcg gcgcgtgtct tatagttgcc gtcatcctta
     3121 aaaaagatgg ttctctcctg tacatagcct tcgggcattg cactcttgaa aaagtcgtgc
     3181 tgtttcatgt gatctgggta gcgcgcaaag cactgaaccc catacccgaa cgtagtgacc
     3241 agtgttggcc atggaacagg tagtttaccc gtggtgcaaa taaatttaag ggtcagcttg
     3301 ccatatgttg cgtcgccttc accctctcca ctgacagaga acttgtgccc attaacatca
     3361 ccgtctaatt ccacgaggat tgggacaact cctgtgaaaa gctcttctcc tttactcata
     3421 gggacaactc cagtgaaaag ttcttctcct ttactcatat gtatatctcc ttcttaaatc
     3481 tagaggatcc ccgggtaccg agctcgaatt ctgtttcctg tgtgaaattg ttatccgctc
     3541 acaattccac acattatacg agccgatgat taattgtcaa cagctcattt cagaatattt
     3601 gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt gccgttgtat
     3661 tgcgacgaat tgccacgttg tcgcagtgtc tgtactgtgg gttaatgtct gtgatcgccg
     3721 tcgtgagttc gtgtcatccg cgcatgattc agatggtcct ggagatcgtt gcttgtaccg
     3781 gctgtttgtg gctctaccac gtctcatcag tctagatgcg aattcagggg cctgtcagac
     3841 caagtttact catatatact ttagattgat ttctgaaagc gaccaggtgc tcgcggccgc
     3901 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca tttttacact
     3961 gatgaatgtt ccgttgcgct gcccggatta cagccggatc cgggatcata tgacaagatg
     4021 tgtatccacc ttaacttaat gatttttacc aaaatcatta ggggattcat cagtgctcag
     4081 ggtcaacgag aattaacatt ccgtcaggaa agctttaagt tggttctctt ggatcaattt
     4141 gctgacaatg gcgtttacct taccagtaat gtattcaagg ctaatttttt caagttcatt
     4201 ccaaccaatg ataggcatca cttcttggat agggataagg tttttattat tatcaataat
     4261 ataatcaaga taatgttcaa atatactttc taaggcagac caaccatttg ttaaatcagt
     4321 ttttgttgtg atgtaggcat caatcataat taattgctgc ttataacagg cactgagtaa
     4381 ttgtttttta tttttaaagt gatgataaaa ggcacctttg gtcaccaacg cttttcccga
     4441 gatcgatctc atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg
     4501 agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca
     4561 tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg
     4621 acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa
     4681 taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa
     4741 agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc
     4801 gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaagt aacaggggtc
     4861 acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga gttgaggttg
     4921 acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca acaatctttt
     4981 acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg taataaatta
     5041 tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag cgtaaccatg
     5101 tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc ttgttattgt
     5161 gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta cgtttcgatt
     5221 tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc
     5281 aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa aacgctctga
     5341 gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca agtctcggaa
     5401 ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg gtgttcgaat
     5461 ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg agtagatttt
     5521 aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc ctttagagcg
     5581 agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc tcttatagcc
     5641 taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt tccagttttc
     5701 cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt accaacccag
     5761 cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa tgagcggtgt
     5821 ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag ctttctttga
     5881 acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac cgtgtagtgc
     5941 gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc agtcaacaag
     6001 tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt atacagcgag
     6061 tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga tgttatgttt
     6121 tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag ttttacagtc
     6181 aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta attcggggca
     6241 gaattggtaa agagagtcgt gtaaaatatc gagttcgcac atcttgttgt ctgattattg
     6301 atttttcgcg aaaccatttg atcctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6361 cacacattat acgagccgat gattaattgt caacagctca tttcagaata tttgccagaa
     6421 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
     6481 aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
     6541 ccagaagcat tggtgcaccg tgcagtcgat gataagctgt caaacatgag aattcccggg
     6601 agagctcggt acccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc
     6661 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
     6721 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
     6781 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
     6841 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
     6901 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
     6961 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
     7021 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
     7081 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
     7141 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
     7201 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
     7261 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
     7321 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
     7381 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
     7441 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     7501 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     7561 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     7621 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     7681 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7741 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
     7801 ctcactcatt aggcacccca ggctttacac tttatgcttc cgacctgcag atctgcaggt
     7861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8221 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8521 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8881 attgatttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8941 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     9001 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     9061 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     9121 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     9181 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     9241 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     9301 aggatccttt ttgtccggtg ttgggttgaa ggtgaagccg gtcggggccg cagcgggggc
     9361 cggcttttca gccttgcccc cctgcttcgg ccgccgtggc tccggcgtct tgggtgccgg
     9421 cgcgggttcc gcagccttgg cctgcggtgc gggcacatcg gcgggcttgg ccttgatgtg
     9481 ccgcctggcg tgcgagcgga acgtctcgta ggagaacttg accttccccg tttcccgcat
     9541 gtgctcccaa atggtgacga gcgcatagcc ggacgctaac gccgcctcga catccgccct
     9601 caccgccagg aacgcaaccg cagcctcatc acgccggcgc ttcttggccg cgcgggattc
     9661 aacccactcg gccagctcgt cggtgtagct ctttggcatc gtctctcgcc tgtcccctca
     9721 gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca aaacagcagg
     9781 gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt
     9841 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc
     9901 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
     9961 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
    10021 gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac
    10081 caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag
    10141 agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt
    10201 cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg
    10261 catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc
    10321 gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa
    10381 gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact
    10441 tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct
    10501 ccatcaagaa gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga
    10561 ccgagcgcct gggtcacgtg cgcgtcacga actgcgaggc aaacaccctg cccgctgtca
    10621 tggccgaggt gatggcgacc cagcacggca acacccgttc cgaggccgac aagacctatc
    10681 acctgctggt tagcttccgc gcgggagaga agcccgacgc ggagacgttg cgcgcgattg
    10741 aggaccgcat ctgcgctggg cttggcttcg ccgagcatca gcgcgtcagt gccgtgcatc
    10801 acgacaccga caacctgcac atccatatcg ccatcaacaa gattcacccg acccgaaaca
    10861 ccatccatga gccgtatcgg gcctaccgcg ccctcgctga cctctgcgcg acgctcgaac
    10921 gggactacgg gcttgagcgt gacaatcacg aaacgcggca gcgcgtttcc gagaaccgcg
    10981 cgaacgacat ggagcggcac gcgggcgtgg aaagcctggt cggctggatc cggccacgat
    11041 gcgtccggcg tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc
    11101 tctcatgttt cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa
    11161 ctaaaccctc atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt
    11221 cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc
    11281 ctctaaggtt ttaagtttta taag
//
