LOCUS       sequence-439047-        9776 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of human integrin beta1 N562* full-length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: Beta1 N562* full-length
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(482..1070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(562..581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1156..1965)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1879..1898
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1984..2031)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(2457..3056)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2540..2560)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3037..3056
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3066..3565)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3114..3133)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3507..3530
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        3649..3952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3954..4229
                     /label=chicken beta-actin promoter
     intron          4230..5246
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5170..5192
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5254..5273
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7748..7774
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7781..7825
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    9493..9548
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9547..9566)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
       61 ctcccagtca tagctgtccc tcttctctta tggagatctt tgcattttta gacatttaga
      121 agcctatatc ttgttacaga attggaatta cacaaaaatt ctaccatatt ttgaaagctt
      181 aggttgttct gaaaaaaaca atatattgtt ttcctgggta aactaaaagt cccctcgagg
      241 aaaggcccct aaagtgaaac agtgcaaaac gttcaaaaac tgtctggcaa tacaagttcc
      301 actttgacca aaacggctgg cagtaaaagg gttaagaaga ctgtcagcct tgagcggtat
      361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg
      901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1081 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg
     1141 gattttggtc atgagttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1201 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1261 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1321 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1381 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1441 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1501 attcattcgt gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt
     1561 acaaacagga atcgagtgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1621 acctgaatca ggatattctt ctaatacctg gaacgctgtt tttccgggga tcgcagtggt
     1681 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gtggcataaa
     1741 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     1801 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc
     1861 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     1921 ggaatttaat cgcggcctcg acgtttcccg ttggatatgg ctcatttttt acttcctcac
     1981 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcga cactgcgggg
     2041 gctctgtgtg gtaagcaggt cttaaccttt ttactgccaa tgacgcatgg gatacgtcgt
     2101 ggcagtaaaa gggcttaaat gccaacgacg cgtcccatac gttgttggca ttttaattct
     2161 tctctctgca gcggcagcat gtgccgccgc tgcagagagt ttctagcgat gacagcccct
     2221 ctgggcaacg agccgggggg gctgtctttc tttatgtttt aaatgcactg acctcccaca
     2281 ttcccttttt agtaaaatat tcagaaataa tttaaataca tcattgcaat gaaaataaat
     2341 gttttttatt aggcagaatc cagatgctca aggcccttca taatatcccc cagtttagta
     2401 gttggactta gggaacaaag gaacctttaa tagaaattgg acagcaagaa agcgagtcag
     2461 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     2521 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga tgtctccagg
     2581 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     2641 agacccttgc cctggtggtc gggcgacacg ccgacggtgg ccaggaacca cgcgggctcc
     2701 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     2761 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     2821 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     2881 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     2941 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     3001 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtg
     3061 gcggacgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga
     3121 agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg
     3181 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
     3241 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
     3301 tccatctgca cgagactagt gagtcgtgct acttccattt gtcacgtcct gcacgacgcg
     3361 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
     3421 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
     3481 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
     3541 cttgggaaaa gcgcctcccc tacccggtag agaaacttga tctgtcgccg caattcaaac
     3601 ttcgtgaggc tccggtgccc gtcagtgacc tgctatactc tggagacgcg ttacataact
     3661 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3721 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3781 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3841 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3901 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     3961 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     4021 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcgc
     4081 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     4141 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     4201 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     4261 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     4321 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     4381 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     4441 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     4501 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     4561 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     4621 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     4681 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     4741 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     4801 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     4861 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     4921 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     4981 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     5041 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     5101 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     5161 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     5221 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     5281 atcattttgg caaagaattc ggcttgatcg aagccgtctc aggggagaga ccacacccaa
     5341 gctgtctaga gccgccacca tgcctttgct cctcctgttg cccttgctct gggctggcgc
     5401 cctcgcgcag acggacgaga atcgatgttt gaaagcgaat gcaaaaagct gcggagagtg
     5461 cattcaggcc ggaccgaatt gtggatggtg tacaaattcg acatttttgc aagagggaat
     5521 gcccacgtcg gcccgatgtg atgacctcga ggccttgaaa aagaaaggat gtccaccgga
     5581 tgacatcgag aacccacggg gctcaaagga catcaaaaag aacaagaatg tcacaaaccg
     5641 ctcgaaggga actgctgaaa agcttaaacc ggaggacatc acgcagatcc agccccaaca
     5701 gctggtgctc cgattgcggt ccggtgagcc acagacgttt actctcaagt tcaagagagc
     5761 ggaggattac ccgatcgatc tttactatct tatggacctt tcgtactcca tgaaggatga
     5821 tttggaaaat gtcaagagcc tcggtactga cttgatgaat gaaatgcgcc gcattaccag
     5881 cgatttccga atcggattcg gatcatttgt ggaaaagacc gtcatgccct acatttcgac
     5941 cactccggca aagttgcgca acccgtgcac ctcagaacag aactgcacct cgcctttttc
     6001 gtataagaac gtcctcagct tgacaaacaa gggtgaagtg ttcaatgagc tggtcggaaa
     6061 gcaaagaatc tcgggaaacc tcgatagccc ggagggaggg tttgatgcaa tcatgcaagt
     6121 ggcggtatgc gggtccctta ttggctggag aaacgtgacc agactcctgg tgttttccac
     6181 tgatgcggga tttcatttcg ccggagacgg gaaactggga gggattgtac ttccgaatga
     6241 cggacagtgt catttggaga acaacatgta tacgatgtcc cactactatg actacccctc
     6301 gatcgcgcac ctcgtccaaa aactgagcga aaacaatatc cagactatct ttgcggtcac
     6361 ggaggagttt cagcccgtgt ataaagaact gaaaaacttg atccctaagt ccgcagtcgg
     6421 aacgctgtcg gccaacagct cgaatgtgat ccagttgatc atcgatgcgt ataactcact
     6481 ttcatcagag gtcatccttg agaacggaaa gcttagcgaa ggagtgacaa tctcgtacaa
     6541 atcatattgc aagaatggag tgaatggcac cggcgagaat ggccggaaat gttcaaacat
     6601 ttccatcggc gacgaggtcc agtttgagat cagcattact tccaataagt gccctaagaa
     6661 agactcggac tcattcaaaa ttcggcctct cgggtttacc gaagaggtag aggtgatcct
     6721 ccagtacatt tgtgaatgcg agtgtcaatc agagggtatc cccgaaagcc ccaagtgtca
     6781 tgagggtaac ggaacgttcg aatgcggggc atgtagatgt aacgaaggtc gcgtcggaag
     6841 gcactgcgaa tgctcgactg atgaggtaaa ctcggaagac atggatgcgt attgccgaaa
     6901 agagaattcg agcgaaatct gctcaaacaa cggggaatgt gtgtgcggac aatgtgtctg
     6961 ccggaaacgg gataacacaa atgagatcta ctccggaaag ttttgcgaat gcgacaattt
     7021 caactgtgac aggtccaacg gtcttatctg tggtggtaat ggagtctgca agtgccgcgt
     7081 ctgcgagtgt tagccgaatt acacaggctc agcgtgtgat tgcagcctcg acacgtcaac
     7141 atgcgaggcg tccaacgggc agatttgtaa cgggcgaggg atttgcgagt gtggggtgtg
     7201 caaatgcact gaccccaaat tccaggggca gacgtgcgag atgtgccaga cgtgtcttgg
     7261 tgtctgtgcg gagcataagg aatgcgtgca gtgcagagcg ttcaacaaag gggaaaagaa
     7321 agatacgtgt acgcaagaat gttcgtattt caatatcacg aaggtggagt cgagggataa
     7381 gctcccgcag ccagtacagc cggaccccgt cagccattgc aaggaaaagg acgtggatga
     7441 ttgctggttc tatttcacat actccgtgaa tggtaataat gaggtaatgg tacatgtcgt
     7501 ggagaacccc gaatgcccca cgggtcccga catcattccc atagtagctg gagtcgtggc
     7561 aggtatcgtg cttatcggtc tggcgttgtt gttgatctgg aaactcttga tgatcatcca
     7621 tgaccgcaga gagtttgcca agtttgagaa agagaagatg aatgcaaaat gggacactgg
     7681 cgaaaaccca atatacaaaa gcgctgttac aacagtggtg aaccccaagt acgagggcaa
     7741 agctagctac ccatacgatg ttccagatta cgctggcagc gggttgaatg atattttcga
     7801 ggctcagaag atagaatggc atgaatgata atcgtggaac agtacgaacg cgccgagggc
     7861 cgccactcca ccggcggcat ggacgagctg tacaagtgat aaagtacttg ataagctctt
     7921 caggttgagg tctctaaaag cgtcttcctc ttctcatcac atcatatcaa ggttatatac
     7981 catcaatatt gccacagatg ttacttagcc ttttaatatt tctctaattt agtgtatatg
     8041 caatgatagt tctctgattt ctgagattga gtttctcatg tgtaatgatt atttagagtt
     8101 tctctttcat ctgttcaaat ttttgtctag ttttattttt tactgatttg taagacttct
     8161 ttttataatc tgcatattac aattctcttt actggggtgt tgcaaatatt ttctgtcatt
     8221 ctatggcctg acttttctta atggtttttt aattttaaaa ataagtctta atattcatgc
     8281 aatctaatta acaatctttt ctttgtggtt aggactttga gtcataagaa atttttctct
     8341 acactgaagt catgatggca tgcttctata ttattttcta aaagatttaa agttttgcct
     8401 tctccattta gacttataat tcactggaat ttttttgtgt gtatggtatg acatatgggt
     8461 tcccttttat tttttacata taaatatatt tccctgtttt tctaaaaaag aaaaagatca
     8521 tcattttccc attgtaaaat gccatatttt tttcataggt cacttacata tatcaatggg
     8581 tctgtttctg agctctactc tattttatca gcctcactgt ctatccccac acatctcatg
     8641 ctttgctcta aatcttgata tttagtggaa cattctttcc cattttgttc tacaagaata
     8701 tttttgttat tgtctttggg ctttctatat acattttgaa atgaggttga caagttaata
     8761 acaggcctat tgattggaaa gtttgtcaac gaattgtggg tcttttgggg tttgctgccc
     8821 cttttacgca atgtggatat cctgctttaa tgcctttata tgcatgtata caagcaaaac
     8881 aggcttttac tttctcgcca acttacaagg cctttctcag taaacagtat atgacccttt
     8941 accccgttgc tcggcaacgg cctggtctgt gccaagtgtt tgctgacgca acccccactg
     9001 gttggggctt ggccataggc catcagcgca tgcgtggaac ctttgtgtct cctctgccga
     9061 tccatactgc ggaactccta gccgcttgtt ttgctcgcag caggtctgga gcaaacctca
     9121 tcgggaccga caattctgtc gtactctccc gcaagtatac atcgtttcca tggctgctag
     9181 gctgtgctgc caactggatc ctgcgcggga cgtcctttgt ttacgtcccg tcggcgctga
     9241 atcccgcgga cgacccctcc cggggccgct tggggctcta ccgcccgctt ctccgtctgc
     9301 cgtaccgtcc gaccacgggg cgcacctctc tttacgcgga ctccccgtct gtgccttctc
     9361 atctgccgga ccgtgtgcac ttcgcttcac ctctgcacgt cgcatggagg ccaccgtgaa
     9421 cgcccaccgg aacctgccca aggtcttgca taagaggact cttggacttt cagcaatgtc
     9481 atctcagtgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     9541 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     9601 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     9661 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     9721 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tcttta
//
