LOCUS       sequence-439050-       10425 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of human integrin beta1 Q280* K418* ectodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: Beta1 Q280* K418* ectodomain
FEATURES             Location/Qualifiers
     source          1..10425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1136..1191
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1190..1209)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(1901..2489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1981..2000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2575..3384)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3298..3317
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3403..3450)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(3876..4475)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3959..3979)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4456..4475
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4485..4984)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4533..4552)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4926..4949
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        5068..5371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5373..5648
                     /label=chicken beta-actin promoter
     intron          5649..6665
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6589..6611
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6673..6692
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8957..9001
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9002..9025
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9116..9142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9149..9178
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             9200..9256
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9257..9967
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9402..9420)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9670..9689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcatgcttct atattatttt ctaaaagatt taaagttttg ccttctccat ttagacttat
       61 aattcactgg aatttttttg tgtgtatggt atgacatatg ggttcccttt tattttttac
      121 atataaatat atttccctgt ttttctaaaa aagaaaaaga tcatcatttt cccattgtaa
      181 aatgccatat ttttttcata ggtcacttac atatatcaat gggtctgttt ctgagctcta
      241 ctctatttta tcagcctcac tgtctatccc cacacatctc atgctttgct ctaaatcttg
      301 atatttagtg gaacattctt tcccattttg ttctacaaga atatttttgt tattgtcttt
      361 gggctttcta tatacatttt gaaatgaggt tgacaagtta ataacaggcc tattgattgg
      421 aaagtttgtc aacgaattgt gggtcttttg gggtttgctg ccccttttac gcaatgtgga
      481 tatcctgctt taatgccttt atatgcatgt atacaagcaa aacaggcttt tactttctcg
      541 ccaacttaca aggcctttct cagtaaacag tatatgaccc tttaccccgt tgctcggcaa
      601 cggcctggtc tgtgccaagt gtttgctgac gcaaccccca ctggttgggg cttggccata
      661 ggccatcagc gcatgcgtgg aacctttgtg tctcctctgc cgatccatac tgcggaactc
      721 ctagccgctt gttttgctcg cagcaggtct ggagcaaacc tcatcgggac cgacaattct
      781 gtcgtactct cccgcaagta tacatcgttt ccatggctgc taggctgtgc tgccaactgg
      841 atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc tgaatcccgc ggacgacccc
      901 tcccggggcc gcttggggct ctaccgcccg cttctccgtc tgccgtaccg tccgaccacg
      961 gggcgcacct ctctttacgc ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg
     1021 cacttcgctt cacctctgca cgtcgcatgg aggccaccgt gaacgcccac cggaacctgc
     1081 ccaaggtctt gcataagagg actcttggac tttcagcaat gtcatctcag tggctaataa
     1141 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1201 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1261 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     1321 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     1381 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     1441 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     1501 cttctcttat ggagatcttt gcatttttag acatttagaa gcctatatct tgttacagaa
     1561 ttggaattac acaaaaattc taccatattt tgaaagctta ggttgttctg aaaaaaacaa
     1621 tatattgttt tcctgggtaa actaaaagtc ccctcgagga aaggccccta aagtgaaaca
     1681 gtgcaaaacg ttcaaaaact gtctggcaat acaagttcca ctttgaccaa aacggctggc
     1741 agtaaaaggg ttaagaagac tgtcagcctt gagcggtatc agctcactca aaggcggtaa
     1801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2281 ggtatgtagg cggtgctaca gagttcttga agtggtgggc taactacggc tacactagaa
     2341 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2521 acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg attttggtca tgagttagaa
     2581 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     2641 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     2701 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2761 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     2821 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     2881 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     2941 agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgagtgcaa
     3001 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     3061 taatacctgg aacgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
     3121 agtacggata aaatgcttga tggtcggaag tggcataaat tccgtcagcc agtttagtct
     3181 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     3241 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
     3301 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     3361 cgtttcccgt tggatatggc tcatttttta cttcctcacc ttgtcgtatt atactatgcc
     3421 gatatactat gccgatgatt aattgtcgac actgcggggg ctctgtgtgg taagcaggtc
     3481 ttaacctttt tactgccaat gacgcatggg atacgtcgtg gcagtaaaag ggcttaaatg
     3541 ccaacgacgc gtcccatacg ttgttggcat tttaattctt ctctctgcag cggcagcatg
     3601 tgccgccgct gcagagagtt tctagcgatg acagcccctc tgggcaacga gccggggggg
     3661 ctgtctttct ttatgtttta aatgcactga cctcccacat tcccttttta gtaaaatatt
     3721 cagaaataat ttaaatacat cattgcaatg aaaataaatg ttttttatta ggcagaatcc
     3781 agatgctcaa ggcccttcat aatatccccc agtttagtag ttggacttag ggaacaaagg
     3841 aacctttaat agaaattgga cagcaagaaa gcgagtcagg caccgggctt gcgggtcatg
     3901 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     3961 tcgtagaagg ggaggttgcg gggcgcggat gtctccagga aggcgggcac cccggcgcgc
     4021 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     4081 ggcgacacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     4141 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     4201 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     4261 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     4321 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
     4381 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
     4441 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg cggacgaaag gcccggagat
     4501 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
     4561 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
     4621 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
     4681 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
     4741 agtcgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
     4801 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
     4861 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
     4921 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
     4981 acccggtaga gaaacttgat ctgtcgccgc aattcaaact tcgtgaggct ccggtgcccg
     5041 tcagtgacct gctatactct ggagacgcgt tacataactt acggtaaatg gcccgcctgg
     5101 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     5161 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     5221 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     5281 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     5341 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     5401 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     5461 ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
     5521 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
     5581 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
     5641 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
     5701 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
     5761 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc
     5821 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg
     5881 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct
     5941 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg
     6001 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc
     6061 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
     6121 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta
     6181 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     6241 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga
     6301 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     6361 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     6421 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     6481 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     6541 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     6601 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     6661 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg
     6721 gcttgatcga agccgtctca ggggagagac cacacccaag ctgtctagag ccgccaccat
     6781 gcctttgctc ctcctgttgc ccttgctctg ggctggcgcc ctcgcgcaga cggacgagaa
     6841 tcgatgtttg aaagcgaatg caaaaagctg cggagagtgc attcaggccg gaccgaattg
     6901 tggatggtgt acaaattcga catttttgca agagggaatg cccacgtcgg cccgatgtga
     6961 tgacctcgag gccttgaaaa agaaaggatg tccaccggat gacatcgaga acccacgggg
     7021 ctcaaaggac atcaaaaaga acaagaatgt cacaaaccgc tcgaagggaa ctgctgaaaa
     7081 gcttaaaccg gaggacatca cgcagatcca gccccaacag ctggtgctcc gattgcggtc
     7141 cggtgagcca cagacgttta ctctcaagtt caagagagcg gaggattacc cgatcgatct
     7201 ttactatctt atggaccttt cgtactccat gaaggatgat ttggaaaatg tcaagagcct
     7261 cggtactgac ttgatgaatg aaatgcgccg cattaccagc gatttccgaa tcggattcgg
     7321 atcatttgtg gaaaagaccg tcatgcccta catttcgacc actccggcaa agttgcgcaa
     7381 cccgtgcacc tcagaacaga actgcacctc gcctttttcg tataagaacg tcctcagctt
     7441 gacaaacaag ggtgaagtgt tcaatgagct ggtcggaaag caaagaatct cgggaaacct
     7501 cgatagcccg gagggagggt ttgatgcaat catgcaagtg gcggtatgcg ggtcccttat
     7561 tggctggaga aacgtgacca gactcctggt gttttccact gatgcgggat ttcatttcgc
     7621 cggagacggg aaactgggag ggattgtact tccgaatgac ggatagtgtc atttggagaa
     7681 caacatgtat acgatgtccc actactatga ctacccctcg atcgcgcacc tcgtccaaaa
     7741 actgagcgaa aacaatatcc agactatctt tgcggtcacg gaggagtttc agcccgtgta
     7801 taaagaactg aaaaacttga tccctaagtc cgcagtcgga acgctgtcgg ccaacagctc
     7861 gaatgtgatc cagttgatca tcgatgcgta taactcactt tcatcagagg tcatccttga
     7921 gaacggaaag cttagcgaag gagtgacaat ctcgtacaaa tcatattgca agaatggagt
     7981 gaatggcacc ggcgagaatg gccggaaatg ttcaaacatt tccatcggcg acgaggtcca
     8041 gtttgagatc agcattactt ccaataagtg ccctaagtag gactcggact cattcaaaat
     8101 tcggcctctc gggtttaccg aagaggtaga ggtgatcctc cagtacattt gtgaatgcga
     8161 gtgtcaatca gagggtatcc ccgaaagccc caagtgtcat gagggtaacg gaacgttcga
     8221 atgcggggca tgtagatgta acgaaggtcg cgtcggaagg cactgcgaat gctcgactga
     8281 tgaggtaaac tcggaagaca tggatgcgta ttgccgaaaa gagaattcga gcgaaatctg
     8341 ctcaaacaac ggggaatgtg tgtgcggaca atgtgtctgc cggaaacggg ataacacaaa
     8401 tgagatctac tccggaaagt tttgcgaatg cgacaatttc aactgtgaca ggtccaacgg
     8461 tcttatctgt ggtggtaatg gagtctgcaa gtgccgcgtc tgcgagtgta atccgaatta
     8521 cacaggctca gcgtgtgatt gcagcctcga cacgtcaaca tgcgaggcgt ccaacgggca
     8581 gatttgtaac gggcgaggga tttgcgagtg tggggtgtgc aaatgcactg accccaaatt
     8641 ccaggggcag acgtgcgaga tgtgccagac gtgtcttggt gtctgtgcgg agcataagga
     8701 atgcgtgcag tgcagagcgt tcaacaaagg ggaaaagaaa gatacgtgta cgcaagaatg
     8761 ttcgtatttc aatatcacga aggtggagtc gagggataag ctcccgcagc cagtacagcc
     8821 ggaccccgtc agccattgca aggaaaagga cgtggatgat tgctggttct atttcacata
     8881 ctccgtgaat ggtaataatg aggtaatggt acatgtcgtg gagaaccccg aatgccccac
     8941 gggtccagac gctagcgggt tgaatgatat tttcgaggct cagaagatag aatggcatga
     9001 acttgaggtg cttttccaag gtcccgcgca atgcaagaag aaattgcaag ccctcaaaaa
     9061 aaagaacgcg cagctcaaat ggaaattgca agcccttaag aagaagctgg cacaataccc
     9121 atacgatgtt ccagattacg ctggcggcca ccatcaccac catcatcatc atcaccacgg
     9181 taccagtact gggagtgggg ccaccaactt ttctctgctc aagcaagccg gggatgttga
     9241 agaaaatcca gggccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     9301 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     9361 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     9421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     9481 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     9541 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     9601 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     9661 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     9721 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     9781 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     9841 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     9901 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     9961 caagtgataa agtacttgat aagctcttca ggttgaggtc tctaaaagcg tcttcctgtt
    10021 ctcatcacat catatcaagg ttatatacca tcaatattgc cacagatgtt acttagcctt
    10081 ttaatatttc tctaatttag tgtatatgca atgatagttc tctgatttct gagattgagt
    10141 ttctcatgtg taatgattat ttagagtttc tctttcatct gttcaaattt ttgtctagtt
    10201 ttatttttta ctgatttgta agacttcttt ttataatctg catattacaa ttctctttac
    10261 tggggtgttg caaatatttt ctgtcattct atggcctgac ttttcttaat ggttttttaa
    10321 ttttaaaaat aagtcttaat attcatgcaa tctaattaac aatcttttct ttgtggttag
    10381 gactttgagt cataagaaat ttttctctac actgaagtca tgatg
//
