LOCUS       sequence-43945-e         888 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS0a-RFP
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             164..175
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(184..218)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        259..363
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(582..601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggacaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga cgaccttcac
       61 cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga actttgaaac
      121 gcatgaactc tttgataacg tcttcggagg aagccatcta gtatttctcc tctttcacta
      181 gtagctagca cagtacctag gactgagcta gctgtcaaag atctcatgaa ttcaggtggc
      241 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      301 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      361 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      421 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      481 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      541 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      601 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      661 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      721 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      781 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      841 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgag
//
