LOCUS       sequence-439559-        5136 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT2AR isoform D coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR...
FEATURES             Location/Qualifiers
     source          1..5136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             44..91
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             125..172
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             188..235
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             251..298
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             314..361
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             377..424
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             440..487
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             491..517
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1755..1771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1755..1771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1768..1790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1943..2531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2023..2042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2648..2672)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2772..3563,3564..3632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3395..3414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3633..3737)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3836..3858
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3850..3867
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3851..3867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgatggagga agcggaggcg acggaggcag cggaggaggc agccgggacc acatggtgct
       61 gcacgagtac gtgaacgccg ccggcatcac tagcggaggc gacggaggca gcggaggagg
      121 cagccgggac cacatggtgc tgcacgagta cgtgaacgcc gccggcatca ctagcggtag
      181 tggcggccgt gaccacatgg tccttcatga gtatgtaaat gctgctggga ttacaggtgg
      241 ctctggaggt agagatcata tggttctcca cgaatacgtt aacgccgcag gcatcactgg
      301 cggtagtgga ggacgcgacc atatggtact acatgaatat gtcaatgcag ccggaataac
      361 cggagggtcc ggaggccggg atcacatggt gctgcatgag tatgtgaacg cggcgggtat
      421 aactggtggg tcgggcggac gagaccatat ggtgcttcac gaatacgtaa acgcagctgg
      481 cattactagt tacccatacg atgttccaga ttacgcttaa ctctagacct aataggaaaa
      541 cacctgaaga cataattacg tggtgctgac ttactgcaaa ttatgattag ataattccca
      601 aggcgagagt ccacgatggc actgagggtg ggcgaaaggt ttttaactca aataatagaa
      661 agctaaaaaa aaaatctctt aagttgttta tcgatagaaa ttcgctagac aagtaatacg
      721 aatctagcat aatataatat ataatgtcgg gcaaagtata ttttttaaaa gttctgttca
      781 actaaactaa acaaatgttt ttcatcaagt aatcttatcg ttgaaatgct tcattatcat
      841 ctgttgtgta agattatgat tatgtaagct actgagaaat cctgatttag aaatcataat
      901 cataaaggaa ttcgtaaagg aaaggaatca taaaggaatt cagaattcat gagcgattta
      961 gatcatatgc cacgtgagat agtagttcac aagcctgcac gtctcacaat attaataaaa
     1021 tagagcgata aaatcagtcg acctaaggac caaggacaag aggtaatgaa cgaataagag
     1081 cttcgctttt ttggcatttt ttgtatgtcg caaaaaactt cataaaagtg tatgaatttc
     1141 taatgaggaa ggggtccccg gccaaactac ccatgggaaa tatgacaagt ttttcccctg
     1201 tgctactcgt acaaaaaata aaggtgtttt ctttacaaat tataagttgg acatttctca
     1261 tattttgttt cctaccattt ctccaggcgc cactcagaaa ttgaaaaact tttggccatt
     1321 tcgatttgag tttttcttct aaatagttta tcgctgctga cctgttccta agggtcagat
     1381 gtggtcgctt acttgatggt tttgtagaac cgggcattta aaatatcttt aacatacttc
     1441 gctaaaaaat catatagtaa ttcaactaac taacggctta aatagttgta tataaaaata
     1501 taaaagttat taactaagca tgattatttt agaacttttt actctttcat cacctatgta
     1561 cgttggatcg gtgatacggc ctcttagatt ggcaaagttc catatactaa taagaccttt
     1621 tctctgaatt cactgaattt cagatcagat tttattatgg gcagctcaga ggtcccccat
     1681 tgaagcggcc gcaattatag gctaggtgga ggctcagtga tgataagtct gcgatggtgg
     1741 atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac
     1801 aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta
     1861 tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag
     2581 ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc tcgtgatacc
     2641 aattcggagc ctgctttttt gtacaaactt gttgataatg gcaattcaag gatcttcacc
     2701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2881 ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc tccagattta
     2941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3241 gtgttatcac tcatggttat ggtagcactg cataattctc ttactgtcat gccatccgta
     3301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3721 caaatagggg ttccgcgcac atttccccga aaagtgccag atacctgaaa caaaacccat
     3781 cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg gttttcccag
     3841 tcacgacgtt gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaaggaagtg
     3901 ccattccgcc tgacctaatt atgctagcca ttgtttgggt aatggccatg atggtatcca
     3961 gttcaataac cgttcttggt aagatacaga acggatgaat tgaaacgtgg ctacaaaagt
     4021 ctatttcctg tgcatttagg tctggtcaat gagaagaaca taatgcccga gcctaacata
     4081 tgcgttatca acaatcgcgc ctttttcgtg tttggatctc tggtagcttt ttatataccc
     4141 atgctgatga tggttaccac atacgcattg acgattcccc tcctccggaa gaaagcgcga
     4201 tttgccgccg agcacccgga aagtgaactt tttcgcaggt aatttcatac tcgttatatg
     4261 gctcccagtt aatttccaag aaaaatcgta attgtaaaca tactgaaata atataaaaag
     4321 agagtttctc gcctgtcata tacccattac ttagccaaaa gtaaactggc aagaaaaaaa
     4381 tctgcacaca atcgttctgt agatgactac taaatttccg gttaagaact cgctcttcca
     4441 tcatctctcg cttcttcttt taaacacagc aatttgtccc attgattggc caatcgaaac
     4501 tcgaccaagt gaacgaatta gcccttactc aatcaagcat cgcctatccc gctatccctc
     4561 atgctctaaa aatctgtaca ctaaagaaag tcccctaatc gtcaaaagct taactaatag
     4621 atttttcaga aggcattata catttttctg agtgcatcca tgatgctttt acgttcatgt
     4681 ttccaggttg ggtggacgct tcaccctaag gccgcagcac agccagcagc agttgcagat
     4741 gttcagtagc ttttctggca gcaataacaa attcctatca atgggcgacg gcaatcgcaa
     4801 cttcaacact gaaggagaga tggaggaggg tggagctcct agcagaagga gtggcgtaga
     4861 gcccgccgaa cgacctttga tgcaacagcg aacggcgagc agcaggagca tgggcacggt
     4921 tagtttccgt aatgtcgtga acgggactag cggagccgca ggaagtgggc gcaggacgac
     4981 tggcactgcc cacagcagct tccggttctc tggcgccggc atctttcggc actcgtcgtc
     5041 gtctcccgcc tcgagctgcc actccaccag cacgtcgcgt tcgagctcct tctggcgcaa
     5101 acacggcgcc tatccaaacc taatggacag gtacgg
//
