LOCUS       sequence-440255-       10687 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses EYA2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Bergmann T, Liu Y, Skov J, Mogus L, Lee J, Pfisterer U, Handfield 
            LF, Asenjo-Martinez A, Lisa-Vargas I, Seemann SE, Lee JTH, Patikas 
            N, Kornum BR, Denham M, Hyttel P, Witter MP, Gorodkin J, Pers TH, 
            Hemberg M, Khodosevich K, Hall VJ
  TITLE     Production of human entorhinal stellate cell-like cells by forward 
            programming shows an important role of Foxp1 in reprogramming.
  JOURNAL   Front Cell Dev Biol. 2022 Aug 15;10:976549. doi: 
            10.3389/fcell.2022.976549. eCollection 2022.
  PUBMED    36046338
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-08-15"; volume: "10:976549. doi"; pages: " 
            10.3389/fcell.2022.976549. eCollection 2022"
COMMENT     Sequence Label: p-Tet-O-EYA2-Puro
FEATURES             Location/Qualifiers
     source          1..10687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        481..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             697..877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    924..1049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1542..1775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1960..2004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2302..2419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2476..2494,2495..2517,2518..2536,2537..2559,2560..2578,
                     2579..2601,2602..2620,2621..2643,2644..2662,2663..2685,
                     2686..2704,2705..2727,2728..2746)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2779..2817
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2917..2926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4543..4596
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4603..5202
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4603..4622)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5099..5119
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5241..5829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5294..5314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5712..5723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5832..5848)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5833..5849)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6354..6534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6560..6577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6566..6790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6836..7264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6923..6942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7133..7154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7273..7293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7278..7607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7458..7593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7520..7539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7655..7702
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7721..8095
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8225..8346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8262..8281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8316..8335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8395..8411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8395..8411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8408..8430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8419..8435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8443..8449,8450..8467,8468..8473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8488..8509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8626..8643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8797..9385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8877..8896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9556..10347,10348..10416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10179..10198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10417..10521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10596..10615)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      721 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      781 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      841 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      901 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      961 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1021 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1081 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1141 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1201 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1261 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1321 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1381 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2221 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     2281 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2341 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2401 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     2461 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2521 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2581 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2641 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2701 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2761 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccgcggcccc gaattcgcca ccatggtaga actagtgatc
     2941 tcacccagcc tcactgtaaa cagcgattgt ctggataaac tgaagtttaa ccgtgctgac
     3001 gctgctgtgt ggactctgag tgacagacaa ggcatcacca aatcggcccc cctgagagtg
     3061 tcccagctct tctccagatc ttgcccacgt gtcctccccc gccagccttc cacagccatg
     3121 gcagcctacg gccagacgca gtacagtgcg gggatccagc aggctacccc ctatacagct
     3181 tacccacctc cagcacaagc ctatggaatc ccttcctaca gcatcaagac agaagacagc
     3241 ttgaaccatt cccctggcca gagtggattc ctcagctatg gctccagctt cagcacctca
     3301 cccactggac agagcccata cacctaccag atgcacggca caacagggtt ctatcaagga
     3361 ggaaatggac tgggcaacgc agccggtttc gggagtgtgc accaggacta tccttcctac
     3421 cccggcttcc cccagagcca gtacccccag tattacggct catcctacaa ccctccctac
     3481 gtcccggcca gcagcatctg cccttcgccc ctctccacgt ccacctacgt cctccaggag
     3541 gcatctcaca acgtccccaa ccagagttcc gagtcacttg ctggtgaata caacacacac
     3601 aatggacctt ccacaccagc gaaagaggga gacacagaca ggccgcaccg ggcctccgac
     3661 gggaagctcc gaggccggtc taagaggagc agtgacccgt ccccggcagg ggacaatgag
     3721 attgagcgtg tgttcgtgtg ggacttggat gagacaataa ttatttttca ctccttactc
     3781 acggggacat ttgcatccag atacgggaag gacaccacga cgtccgtgcg cattggcctt
     3841 atgatggaag agatgatctt caaccttgca gatacacatc tgttcttcaa tgacctggag
     3901 gattgtgacc agatccacgt tgatgacgtc tcatcagatg acaatggcca agatttaagc
     3961 acatacaact tctccgctga cggcttccac agttcggccc caggagccaa cctgtgcctg
     4021 ggctctggcg tgcacggcgg cgtggactgg atgaggaagc tggccttccg ctaccggcgg
     4081 gtgaaggaga tgtacaatac ctacaagaac aacgttggtg ggttgatagg cactcccaaa
     4141 agggagacct ggctacagct ccgagctgag ctggaagctc tcacagacct ctggctgacc
     4201 cactccctga aggcactaaa cctcatcaac tcccggccca actgtgtcaa tgtgctggtc
     4261 accaccactc aactaattcc tgccctggcc aaagtcctgc tatatggcct ggggtctgtg
     4321 tttcctattg agaacatcta cagtgcaacc aagacaggga aggagagctg cttcgagagg
     4381 ataatgcaga gattcggcag aaaagctgtc tacgtggtga tcggtgatgg tgtggaagag
     4441 gagcaaggag cgaaaaagca caacatgcct ttctggcgga tatcctgcca cgcagacctg
     4501 gaggcactga ggcacgccct ggagctggag tatttatcta gagagggcag gggaagtctt
     4561 ctaacatgcg gggacgtgga ggaaaatccc ggcccactcg agatgaccga gtacaagccc
     4621 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4681 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4741 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4801 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4861 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4921 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4981 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5041 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5101 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5161 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gattaattaa gctagctaat
     5221 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5281 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5341 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5401 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5461 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5521 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5581 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5641 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5701 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5761 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5821 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5881 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5941 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6001 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6061 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6121 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6181 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6241 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6301 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7261 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7321 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7381 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7561 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7621 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7681 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7741 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7801 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7861 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7921 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7981 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8041 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8101 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8161 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8221 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8341 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9301 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9361 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9421 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9481 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9541 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9601 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9661 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9721 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9781 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9841 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9901 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9961 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10021 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10081 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10141 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10201 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10261 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10321 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10381 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10441 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10501 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10561 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10621 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10681 atttaag
//
