LOCUS       sequence-440462-        7060 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Zhang SQ, Tsiaras WG, Araki T, Wen G, Minichiello L, Klein R, Neel 
            BG
  TITLE     Receptor-specific regulation of phosphatidylinositol 3'-kinase 
            activation by the protein tyrosine phosphatase Shp2.
  JOURNAL   Mol Cell Biol 2002 Jun;22(12):4062-72.
  PUBMED    12024020
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-06"; volume: "22(12)"; pages: "4062-72"
COMMENT     Sequence Label: pBABE-puro SHP2 WT
FEATURES             Location/Qualifiers
     source          1..7060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3390..3410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3395..3724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3575..3710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3734..4333
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3734..3753)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4230..4250
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4554..5023
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5153..5170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5324..5912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5404..5423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6072..6863,6864..6932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6695..6714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6933..7037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acgtaggaat tccgggcggg aggaacatga catcgcggag atggtttcac ccaaatatca
     1441 ctggtgtgga ggcagaaaac ctactgttga caagaggagt tgatggcagt tttttggcaa
     1501 ggcctagtaa aagtaaccct ggagacttca cactttccgt tagaagaaat ggagctgtca
     1561 cccacatcaa gattcagaac actggtgatt actatgacct gtatggaggg gagaaatttg
     1621 ccactttggc tgagttggtc cagtattaca tggaacatca cgggcaatta aaagagaaga
     1681 atggagatgt cattgagctt aaatatcctc tgaactgtgc agatcctacc tctgaaaggt
     1741 ggtttcatgg acatctctct gggaaagaag cagagaaatt attaactgaa aaaggaaaac
     1801 atggtagttt tcttgtacga gagagccaga gccaccctgg agattttgtt ctttctgtgc
     1861 gcactggtga tgacaaaggg gagagcaatg acggcaagtc taaagtgacc catgttatga
     1921 ttcgctgtca ggaactgaaa tacgacgttg gtggaggaga acggtttgat tctttgacag
     1981 atcttgtgga acattataag aagaatccta tggtggaaac attgggtaca gtactacaac
     2041 tcaagcagcc ccttaacacg actcgtataa atgctgctga aatagaaagc agagttcgag
     2101 aactaagcaa attagctgag accacagata aagtcaaaca aggcttttgg gaagaatttg
     2161 agacactaca acaacaggag tgcaaacttc tctacagccg aaaagagggt caaaggcaag
     2221 aaaacaaaaa caaaaataga tataaaaaca tcctgccctt tgatcatacc agggttgtcc
     2281 tacacgatgg tgatcccaat gagcctgttt cagattacat caatgcaaat atcatcatgc
     2341 ctgaatttga aaccaagtgc aacaattcaa agcccaaaaa gagttacatt gccacacaag
     2401 gctgcctgca aaacacggtg aatgactttt ggcggatggt gttccaagaa aactcccgag
     2461 tgattgtcat gacaacgaaa gaagtggaga gaggaaagag taaatgtgtc aaatactggc
     2521 ctgatgagta tgctctaaaa gaatatggcg tcatgcgtgt taggaacgtc aaagaaagcg
     2581 ccgctcatga ctatacgcta agagaactta aactttcaaa ggttggacaa gggaatacgg
     2641 agagaacggt ctggcaatac cactttcgga cctggccgga ccacggcgtg cccagcgacc
     2701 ctgggggcgt gctggacttc ctggaggagg tgcaccataa gcaggagagc atcatggatg
     2761 cagggccggt cgtggtgcac tgcagtgctg gaattggccg gacagggacg ttcattgtga
     2821 ttgatattct tattgacatc atcagagaga aaggtgttga ctgcgatatt gacgttccca
     2881 aaaccatcca gatggtgcgg tctcagaggt cagggatggt ccagacagaa gcacagtacc
     2941 gatttatcta tatggcggtc cagcattata ttgaaacact acagcgcagg attgaagaag
     3001 agcagaaaag caagaggaaa gggcacgaat atacaaatat taagtattct ctagcggacc
     3061 agactagtgg agatcagagc cctctcccgc cttgtactcc aacgccaccc tgtgcagaaa
     3121 tgagagaaga cagtgctaga gtctatgaaa acgtgggcct gatgcaacag cagaaaagtt
     3181 tcagatgaga aaacctgcca aaacttcagc acagaaatag atgtggactt tcaccctctc
     3241 cctaaaaaga tcaagaacag acgcaagaaa gtttatgtga agacagaatt tggatttgga
     3301 aggcttgcaa tgtggttgac taccttttga taagcaaaat ttgaaaccat ttaaagaccg
     3361 gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3481 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3541 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3601 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3661 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3721 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3781 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3841 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3901 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3961 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4021 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4081 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4141 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4201 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4261 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4321 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     4381 ccatgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     4441 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     4501 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     4561 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     4621 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     4681 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     4741 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     4801 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     4861 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     4921 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     4981 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     5041 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     5101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6001 ggatcttcac ctagatcctt ttgcggccgc aaatcaatct aaagtatata tgagtaaact
     6061 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7021 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
