LOCUS       sequence-440865-        9719 bp    DNA     circular SYN 14-APR-2025
DEFINITION  lentiviral vector for expressing human B3GALT5 with D156A and D243A 
            inactivating mutations and C-terminal myc-DDK tag, includes 
            nucleotide change in PAM targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9719)
  TITLE     Fucosylated glycoproteins and fucosylated glycolipids play opposing 
            roles in cholera intoxication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiMutB3GALT5catdead-blast
FEATURES             Location/Qualifiers
     source          1..9719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..207
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     162..182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             225..405
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    452..577
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1070..1303
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1488..1532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1830..1947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2040..2251
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3240..3269
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3288..3311
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3321..3377
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3378..3776
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3801..4389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3854..3874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4272..4283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4392..4408)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4393..4409)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4914..5094
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5120..5137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5126..5350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5396..5824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5483..5502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5693..5714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5833..5853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5838..6167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6018..6153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6080..6099
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6215..6262
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6281..6655
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6785..6906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6822..6841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6876..6895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6955..6971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6955..6971)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6968..6990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6979..6995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7003..7009,7010..7027,7028..7033))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7048..7069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7186..7203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7357..7945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7437..7456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8116..8907,8908..8976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8739..8758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8977..9081)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9156..9175)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9347..9719,1..7)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
       61 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      121 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      181 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      241 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      301 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      361 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      421 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      481 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      541 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      601 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      661 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      721 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      781 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
      841 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
      901 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
      961 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1021 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1081 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1141 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1201 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1261 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1321 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1381 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1441 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1501 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1561 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1621 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1681 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1741 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     1801 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     1861 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     1921 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     1981 tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     2041 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
     2101 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2161 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2221 ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgagg agatctgccg
     2281 ccgcgatcgc catggctttc ccgaagatga gattgatgta tatttgcctt ctggttctgg
     2341 gggctctttg tttgtatttt agcatgtaca gtctaaatcc tttcaaagaa cagtcctttg
     2401 tttacaagaa agacgggaac ttccttaagc tcccagatac agactgcagg cagacacctc
     2461 ccttcctcgt cctgctggtg acctcatccc acaaacagtt ggctgagcgc atggccatcc
     2521 ggcagacgtg ggggaaagag aggacggtga agggaaagca gctgaagaca ttcttcctcc
     2581 tggggaccac cagcagtgca gcggaaacaa aagaggtgga ccaggagagc cagcgacacg
     2641 gggacattat ccagaaggat ttcctagacg tctattacaa tctgaccctg aagaccatga
     2701 tgggcataga atgggtccat cgcttttgtc ctcaggcagc gtttgtgatg aaaacagctt
     2761 cagacatgtt catcaatgtt gactatctga ctgaactgct tctgaagaaa aacagaacaa
     2821 ccaggttttt cactggcttc ttgaaactca atgagtttcc catcaggcag ccattcagca
     2881 agtggtttgt cagtaaatct gaatatccgt gggacaggta cccaccattc tgctccggca
     2941 ccggctacgt gttttctggc gacgtggcga gtcaggtgta caatgtctcc aagagcgtcc
     3001 catacattaa actggaagct gtgtttgtgg ggctctgcct cgaaaggctg aacatcagat
     3061 tggaggagct ccactcccag ccgacctttt ttccaggggg cttacgcttc tccgtatgcc
     3121 tcttcaggag gatcgtggcc tgccacttca tcaagcctcg gactctcttg gactactggc
     3181 aggctctaga gaattcccgg ggggaagatt gtccgcctgt cacgcgtacg cggccgctcg
     3241 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     3301 acgacgataa gggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg
     3361 aagagaatcc tggaccgatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     3421 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     3481 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     3541 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     3601 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     3661 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     3721 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagaat
     3781 tcgatatcaa gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg
     3841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4201 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4381 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     4441 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     4501 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     4561 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4621 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     4681 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     4741 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     4801 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     4861 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     4921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5101 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5161 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5221 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5281 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5341 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5821 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6181 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6241 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6301 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6361 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6421 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     6481 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     6541 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     6601 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     6661 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6721 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6781 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6901 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6961 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7021 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7081 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7141 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7201 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7261 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7321 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7381 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7441 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7501 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7561 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7621 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7681 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7981 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8221 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9121 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9181 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9241 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9301 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     9361 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     9421 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     9481 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     9541 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     9601 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     9661 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctat
//
