LOCUS       sequence-441034-        1434 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Scip expression in Chick.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ritesh KC, de Boer RL, Lin M, Jeannotte L, Philippidou P
  TITLE     Multimodal Hox5 activity generates motor neuron diversity.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 8:2024.02.08.579338. doi: 
            10.1101/2024.02.08.579338.
  PUBMED    38370781
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 8:2024.02.08.579338. doi: 
            10.1101/2024.02.08.579338."
COMMENT     Sequence Label: pCAGGS-V5-Scip
FEATURES             Location/Qualifiers
     source          1..1434
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..68
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1380..1397
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttggcaa agaattcgcc accatgggta agcctatccc taaccctctc ctcggtctcg
       61 attctacggc caccaccgcg cagtatctgc cgcggggccc cggcggcgga gctgggggca
      121 cagggccgct catgcatccc gatgccgccg cggcggcggc agcggcggcg gcggccgagc
      181 ggctgcacgc gggggccgcg taccgcgaag tgcagaagct gatgcaccac gagtggctgg
      241 gcgcgggcgc gggccacccc gtgggcctag cgcaccctca atggctaccc acgggaggag
      301 gcggcggcgg cgactgggcg ggcggcccgc acctggaaca cggcaaggca ggcggtggcg
      361 gtaccggccg agctgacgac ggcggcggtg gcggcggttt ccacgcccgc ctggtgcacc
      421 aaggggcggc ccacgcgggc gcggcatggg cacaaggcgg cacagcgcac cacttgggcc
      481 ccgccatgtc gccgtcgccc ggggccggcg ggggtcacca gccccagccg ctcgggctgt
      541 acgctcaggc ggcctacccc ggtggcggcg gcggcggcct ggccgggatg ctggcggcgg
      601 gaggcggcgg cgcgggaccc ggcctgcacc acgcactgca cgaggacggc cacgaggcac
      661 agctggagcc gtcgccacca ccgcacctgg gcgcacacgg acacgcacac ggacatgcac
      721 acgcgggcgg cctgcacgcg gcggcggcgc acctgcaccc gggcgcgggc ggtggtggct
      781 cgtcggtggg cgagcactcg gacgaggatg ctcccagctc cgacgacctg gagcagttcg
      841 ccaagcagtt caagcaacga cgcatcaagc tgggcttcac ccaggccgac gtgggactgg
      901 cgctgggcac cctctacggt aacgtgttct cgcagaccac catctgccgt ttcgaggccc
      961 tgcagctgag cttcaagaac atgtgcaagc tcaagccgct gctcaacaag tggctggagg
     1021 agaccgactc gtccagcggc agccccacca acctggacaa gatcgcggcg cagggccgca
     1081 agcgcaagaa gcgcacgtcc atcgaggtgg gtgtcaaagg cgcgctcgag agccactttc
     1141 tcaagtgtcc caagccgtct gcgcacgaga tcaccggcct ggccgacagc ctgcaactgg
     1201 agaaggaggt ggtgcgtgtc tggttctgca accggcggca gaaggagaag cgcatgaccc
     1261 ccgcggccgg cgcgggccac ccgcccatgg acgacgttta tgcgcctggg gagctggggc
     1321 ctggcggggg cagcgcgtcg ccaccttctg cgcccccgcc acccccgccg gccgcgctgc
     1381 accaccacca ccaccacaca ctgcccggct ctgtgcagtg agcggccgca ctcc
//
