LOCUS       sequence-441445-       13584 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dox-inducible lentiviral transfer vector with Arg kinase-dead 
            (K281M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13584)
  TITLE     Dynamic regulation of integrin beta-1 phosphorylation supports 
            invasive breast cancer progression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-ABL2(K281M)
FEATURES             Location/Qualifiers
     source          1..13584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3573..3584
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6171..6195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6248..6274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        6360..7571
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7408..7427
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7630..8247,8248..8253,8254..8289,8290..8292,8293..8328,
                     8329..8331,8332..8367,8368..8376)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8414..8987
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8581..8598)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8808..8827
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9003..9797
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9003..9797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9057..9076)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9667..9686
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9974..10562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10027..10047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10445..10456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10637..10870
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10989..11007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11075..11179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11180..11248,11249..12040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11398..11417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12211..12799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12700..12719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12953..12970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13210..13231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13246..13251,13252..13269,13270..13276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13284..13300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13289..13311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13308..13324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13308..13324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13374..13393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagt tggcaccatg gtccttggga cagttctcct tccacctaat
     2761 agttatggca gagatcagga cacttcactt tgctgcctgt gcactgaggc ctcagaatct
     2821 gctctacccg acttaacaga agctttgcat cgtccctatg gttgtgatgt tgaaccccag
     2881 gcactaaatg aggctatcag gtggagctcc aaggagaact tgctcggagc cactgagagt
     2941 gaccctaatc tcttcgttgc actttatgat tttgtagcaa gtggtgataa cacactcagc
     3001 atcactaaag gtgaaaagct acgagtcctt ggttacaacc agaatggtga gtggagtgaa
     3061 gttcgctcta agaatgggca gggctgggtg ccaagcaact acatcacccc agtgaacagc
     3121 ctggaaaaac actcctggta ccatggacct gtgtcacgca gtgcagctga gtatctgctc
     3181 agcagtctaa tcaatggcag cttcctggtg cgagaaagtg agagtagccc tgggcagctg
     3241 tccatctcgc tcaggtacga gggacgtgtg tatcactaca ggatcaatac cactgcagat
     3301 ggcaaggtgt atgtgactgc tgagagccgc ttcagcacct tggcagagct tgtacaccat
     3361 cactccacag tggctgatgg gctggtgaca acattacact acccagcacc caagtgtaat
     3421 aagcctacag tctatggtgt gtcccccatc cacgacaaat gggaaatgga gcgaacagat
     3481 attaccatga agcacaaact tgggggcggt cagtatggag aggtttacgt tggcgtctgg
     3541 aagaaataca gccttacagt tgctgtgatg acattgaagg aagataccat ggaggtagaa
     3601 gaattcctga aagaagctgc agtaatgaag gaaatcaagc atcctaatct ggtacaactt
     3661 ttaggtgtgt gtactttgga gccaccattt tacattgtga ctgaatacat gccatacggg
     3721 aatttgctgg attacctccg agaatgcaac cgagaagagg tgactgcagt tgtgctgctc
     3781 tacatggcca ctcagatttc ttctgcaatg gagtacttag agaagaagaa tttcatccat
     3841 agagatcttg cagctcgtaa ctgcctagtg ggagaaaacc atgtggtaaa agtggctgac
     3901 tttggcttaa gtagattgat gactggagac acttatactg ctcatgctgg agccaaattt
     3961 cctattaagt ggacagcacc agagagtctt gcctacaata ccttctcaat taaatctgac
     4021 gtctgggctt ttggggtatt gttgtgggaa attgctacct atggaatgtc accatatcca
     4081 ggtattgacc tgtctcaggt ctatgaccta ctagaaaaag gatatcgaat ggaacagcct
     4141 gagggatgcc cccctaaggt ttatgaactt atgagagcat gctggaagtg gagccctgcc
     4201 gataggccct cttttgctga aacacaccaa gcttttgaaa ccatgttcca tgactccagc
     4261 atttctgaag aggtagctga ggagcttggg agagccgcct cctcgtcatc tgttgttcca
     4321 tacctgcccc ggctacctat acttccttcc aagactcgga cactgaagaa acaggtggag
     4381 aacaaggaga acattgaagg ggcacaagat gccacagaaa attctgcttc cagtttagca
     4441 ccagggttca tcagaggtgc acaggcctct agtggatccc catcactgcc tcgaaagcaa
     4501 agagacaagt cacccagcag cctcttggaa gatgccaaag agacatgctt caccagggat
     4561 aggaaggggg gcttcttcag ctccttcatg aagaagagaa atgctcccac accccccaaa
     4621 cgcagcagct ccttccgaga aatggagaat cagccccata agaaatacga actcacgggt
     4681 aacttctcat ctgttgcttc tctacagcat gctgatgggt tctctttcac tcctgcccag
     4741 caagaggcga atctggtgcc acccaagtgc tatgggggga gctttgcaca gaggaacctc
     4801 tgtaatgacg acggtggtgg gggtgggggc agtggcactg ctgggggtgg gtggtctggc
     4861 atcacaggct tctttacacc acgcttaatc aaaaagacac tgggcttacg agcaggtaaa
     4921 cccacagcca gtgatgacac ttccaagcct tttccaaggt caaactctac atcttccatg
     4981 tcctcagggc ttccagagca ggataggatg gcaatgaccc ttcccaggaa ctgccagagg
     5041 tccaaactcc agctggaaag gacagtgtcc acctcttctc agccagaaga gaatgtggac
     5101 agggccaatg acatgcttcc aaaaaaatca gaggaaagtg ctgctccaag cagggagaga
     5161 ccaaaagcca agttattgcc cagaggagcc acagctcttc ctctcagaac accctctggg
     5221 gatctagcca ttacagagaa ggaccctcca ggggtgggag tggctggagt ggcagctgcc
     5281 cccaagggta aagagaagaa tggtggggca cgacttggga tggctggagt tccagaggat
     5341 ggagagcagc cgggctggcc ttctccagcc aaggctgccc ccgtcctccc aaccactcac
     5401 aaccacaaag tgccagtcct tatctcaccc actctgaaac acactccagc tgacgtgcag
     5461 ctcattggca cagactctca ggggaataaa ttcaagctct tatctgagca tcaggtcaca
     5521 tcctctggag acaaggaccg accccgacgg gtaaaaccaa agtgtgcccc acccccacca
     5581 ccagtgatga gactactgca gcatccgtcc atctgctcag accctacaga agagccaact
     5641 gccctaactg caggacagtc cacatcagaa acacaggaag gaggaaagaa ggcagctctg
     5701 ggcgcagtgc ccatcagtgg gaaagctggg aggccagtga tgcctccacc tcaagtgcct
     5761 ctgcccacat cttccatctc gccagccaaa atggccaatg gcacagcagg tactaaagtg
     5821 gctctgagaa aaaccaaaca ggccgctgag aaaatctcag cagacaaaat cagcaaagag
     5881 gccctgctgg aatgtgctga cctactgtcc agtgcactca cggaacctgt gcccaacagc
     5941 cagctggtag acactggaca ccagctgctt gactactgct caggctatgt ggactgcatc
     6001 cctcaaactc gcaacaaatt tgccttccga gaggctgtga gcaaactgga actcagcctg
     6061 caggagctac aggtttcttc agcagctgct ggtgtgcccg ggacaaaccc tgtccttaat
     6121 aacttattgt catgtgtaca ggaaatcagt gatgtggtgc agaggtagac acccagcttt
     6181 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     6241 gttcgaatac ccatacgacg tcccagacta cgcttagtaa tgattaatta aactagaaat
     6301 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agtaacccac ccaagatctg
     6361 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     6421 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     6481 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     6541 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     6601 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     6661 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     6721 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     6781 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     6841 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     6901 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     6961 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     7021 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     7081 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     7141 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     7201 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     7261 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     7321 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     7381 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     7441 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     7501 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     7561 ttttgttaga cgaagcttgg taccgagctc ggatctccac cccgtaccgg tcctgcagtc
     7621 gaattcacca tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     7681 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     7741 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     7801 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     7861 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     7921 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     7981 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     8041 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     8101 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     8161 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     8221 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     8281 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     8341 cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcc gcggccgcac
     8401 tagaggaatt ccgcccctct cccccccccc cccctaacgt tactggccga agccgcttgg
     8461 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     8521 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8581 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8641 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8701 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8761 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8821 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8881 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8941 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct taccggtcca
     9001 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9061 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9121 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9181 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9241 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9301 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9361 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9421 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9481 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     9541 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9601 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9661 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9721 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9781 ttgacgagtt cttctgatgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     9841 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     9901 aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc caattcgata tcaagcttat
     9961 cgcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    10021 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    10081 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    10141 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    10201 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    10261 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    10321 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
    10381 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    10441 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10501 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10561 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    10621 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
    10681 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10741 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10801 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10861 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    10921 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
    10981 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    11041 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    11101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    11161 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    11221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    11281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    11341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    11401 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
    11461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    11521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    11581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    11641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    11701 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11881 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    12001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    12061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    12121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    12361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    12661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    12721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    12781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12841 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    12901 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12961 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    13021 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
    13081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    13141 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    13201 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    13261 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    13321 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    13381 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    13441 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    13501 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    13561 aatatgtact acaaacttag tagt
//
