LOCUS       sequence-441605-         143 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pX459 plasmid encoding the sgRNA protospacer sequence 
            "5'-GCATAATGTCACTACAGATC-3' to target the LIG4 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 143)
  TITLE     SelectRepair Knockout: Efficient PTC-free gene knockout through 
            selectable homology-directed DNA repair
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX459_LIG4_Exon3
FEATURES             Location/Qualifiers
     source          1..143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgca taatgtcact
       61 acagatcgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgc
//
