LOCUS       sequence-441612-        5714 bp    DNA     circular SYN 15-APR-2025
DEFINITION  tax-2 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY137
FEATURES             Location/Qualifiers
     source          1..5714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1079..1099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1121..1141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3305..3327)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3427..3446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3524..3542)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3610..3714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3715..3783,3784..4575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3933..3952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4746..5334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5235..5254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5488..5505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5622..5643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5658..5663,5664..5681,5682..5688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5696..5712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5701..5714,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc agcaaaccca
      181 aaggaataaa tattccgatt cgatggaaaa ttgtaaattc attgttttaa atgatttttt
      241 tattttatta cttggcctgt caaataccgg gtttttaatc gataaacgta ttgttgaaaa
      301 atattccaat aaattttgaa ttatatatgg aaaatataaa aatcgactga caatgagtct
      361 ttaatttgaa ttgcttaaaa atctaggatg atggtactgt aactattgtt tttttttatt
      421 tttacgagtc aacaaaaacg aaaccatcag ttatgttcaa tagttgccga tatcagtaga
      481 acattcaaca ttacaagaga caactcgctt gaaaacttgt acagtgcaac aaaataaaca
      541 attaattttg gcagacagat acctatttaa taagcagtta tgaagcatga aaagtcaaaa
      601 tatgatgact gcttggcaac ggacttaatg aatgatcaac acattgggag cataagaggg
      661 attataatag tagcaagaaa gagaaagaga agaagaagaa gcacgcaagg gaggtcatat
      721 caactagtat ttccatttga gagaaggaga tggatagaga aggcttttag aaggttttaa
      781 tatttatcac tggaaattta agccttccta tcatttcttt gcgtctcctt gtatatatct
      841 tcactatatt gtttcctgtt ttcgtaacta ttttgaatcg tttttttcaa acccaagttt
      901 ccatgttcaa ttggattttt acagtttcgt ttcagaaatg tcagaaaaac cggagttttc
      961 cgatcccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
     1021 gaacattttc aggaggaccc ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc
     1081 aaagaagaag cgcaaggttc cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt
     1141 cccgcggatg tcagagctta ttaaggagaa tatgcatatg aaactgtaca tggaaggaac
     1201 cgtggacaac catcacttta agtgcacttc ggagggggaa ggcaaaccgt acgagggcac
     1261 ccagaccatg cgaatcaagg tggtcgaggg aggcccactg cccttcgcct tcgacattct
     1321 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc aggctactag
     1381 cttcctgtac ggcagcaaga cttttattaa tcacacccag ggcatcccgg acttcttcaa
     1441 acagtcgttc cctgagggct tcacatggga gcgtgtgacc acatacgaag acggtggcgt
     1501 tctcaccgct acccaggaca ccagcctcca ggacggttgc ctcatatata atgtggtaag
     1561 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagaaga tccggggagt
     1621 aaacttcaca tccaatggtc cggttatgca gaagaaaaca ctcggctggg aggccttcac
     1681 cgagacgctg tatccggctg acggaggcct ggaaggtcga aacgacatgg ccctgaagct
     1741 cgtcggcggt agccatctca tcgcaaacgc taagaccaca tatagatcca agaaaccggt
     1801 aagtttaaac atgattttac taactaacta atctgattta aattttcagg ctaagaacct
     1861 taaaatgccg ggagtctact atgttgacta cagactggaa cggatcaagg aggccaacaa
     1921 cgagacctac gtcgagcagc acgaggttgc agttgccaga tactgcgacc tcccgagcaa
     1981 actgggtcac aagcttaatc ctaggatacc ggtgggtgaa gacccaccaa agccatctgc
     2041 caagggagcc aagaaggccg ccaagaccgt tacgaagcca aaggacggaa agaagagacg
     2101 tcatgcccgt aaggaatcat actccgtcta catctaccgt gtcctcaagc aagttcatcc
     2161 agacactgga gtttcctcca aagccatgtc tatcatgaac tcttttgtca acgatgtctt
     2221 cgagcgtatt gctgctgaag catcccgtct tgctcactac aacaagcgtt ccacaatctc
     2281 atcccgcgaa attcagaccg ctgtccgtct gatccttcca ggagagcttg ccaagcacgc
     2341 cgtgtctgag ggaaccaagg ccgttaccaa gtacacttcc agcaagtagg aattccaact
     2401 gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc
     2461 atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat
     2521 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     2581 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     2641 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     2701 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     2761 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     2821 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     2881 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     2941 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     3001 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     3061 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     3121 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     3181 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     3241 tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     3301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     3421 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     3481 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
     3541 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5701 agcggataac aatt
//
