LOCUS       sequence-442077-        6037 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A recombinant AAV2 plasmid encoding the ZipACR I151T mutant and soma
            targeting with KV2.1 motif..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Mermet-Joret N, Moreno A, Zbela A, Ellendersen BE, Krauth N, von 
            Philipsborn A, Piriz J, Lin JY, Nabavi S
  TITLE     Dual-color optical activation and suppression of neurons with high 
            temporal precision
  JOURNAL   eLife 2023 12:RP90327
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 2023 
            12:RP90327"
COMMENT     Sequence Label: AAV-hSyn-ZipV-mScarlet-KV2.1
FEATURES             Location/Qualifiers
     source          1..6037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     rep_origin      complement(737..1325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(817..836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1496..2287,2288..2356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2119..2138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2357..2461)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2487..2942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2624..2645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     repeat_region   3014..3143
                     /label=AAV2 ITR
     promoter        3225..3673
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4636..5331
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4769..4787)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5037..5056
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5591..5611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
       61 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
      121 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      181 gcgctctatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      241 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      301 cactaaatcn rraccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      361 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      421 tagcggtcac gctgcgcgta accaccacac ccgccgcgcy taatgcgccg ctacagggcg
      481 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      541 ssstattnsg scrsycttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2461 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     2521 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2581 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2641 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2701 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2761 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2821 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2881 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2941 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3001 gctattacgc cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     3061 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     3121 caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac
     3181 gtagccatgc tctaggaaga tctctgcaga gggccctgcg tatgagtgca agtgggtttt
     3241 aggaccagga tgaggcgggg ntgggggtgc ctacctgacg accgaccccg acccactgga
     3301 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
     3361 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
     3421 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
     3481 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
     3541 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
     3601 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
     3661 cctgagagcg cagtcgaatt caagcttgga tccatggccg ccctccatca ggctctggag
     3721 gagctgggac agggagccag caggcacctc ctgtacagag cccccgagga gatggtgtat
     3781 ggcaagtggg tggaggccaa ccctaggtgc atcgaactgc acgaccactc cgactacgtg
     3841 tacgtgttcc agctgtgctt cgccgtggtg tgcgcctgcc aggtgatctt catgttcacc
     3901 agggccccca acgtgggctg ggaagccatc tatctgcccc tggccgaggt cgtgacctac
     3961 agcattgccg ccaacggaga gggcgtgctg aggatggccg acggcaggta cttcaatttc
     4021 gccaaactgg ccggatgggc cgtgtgctgc cccattatgc tcatccagat cggaggcatg
     4081 gcccagatca agtacaggac catccccctg aacaacgtgg tgctggccgc tagcctgaat
     4141 aggaccatct tcggcatggc cagcgccatc acagcctccg accctgctag atggggcttt
     4201 tacttctgcg cctggatctg ttacctgacc gaggtcggca tcaccctgac catcatggcc
     4261 gtggccatca gcgacttcag caagatcaag acagagctcg gccagtgggt ggtgggccgt
     4321 attcaaacca tgaggatcat ctttctggtc gcctggacca gcttccccgt cgtgtgggtg
     4381 ctgggctaca ccggattctg cgtgatccac gaggactaca tcgccctgct gtatctgttc
     4441 gccgacctgc tctccaagaa cacctgggga gtgatgatgt ggcataccac ctgggtgaaa
     4501 ctcaacggca aatgggacag ggaatttgcc gccgccggag gacacgaggc tctgaagaag
     4561 gccctggagc aggacgtgga aatcggcgcc ggcgagaaga accagaacca gctgaccgcc
     4621 aggcaggcgg ccgcaatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     4681 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     4741 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4801 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcaccaag
     4861 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     4921 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     4981 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     5041 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     5101 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg gcgctacctg
     5161 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     5221 gtggaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     5281 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gcagtcccaa
     5341 cccatactga acacgaagga aatggctcct caaagtaaac cgcccgaaga actcgaaatg
     5401 agttcaatgc catcacctgt ggctccgctt cccgcccgca cggaaggtgt catcgacatg
     5461 cgaagcatgt ctagtattga ctcatttatt agctgcgcca ccgatttccc tgaagctact
     5521 cggttctaat ctagaataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5581 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5641 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
     5701 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5761 tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg
     5821 taaccatcac tagttaggct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5881 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5941 tcagggggag atgtgggagg ttttttaaaa tcgataagga tcttcctaga gcatggctac
     6001 gtagataagt agcatggcgg gttaatcatt aactaca
//
