LOCUS       sequence-442086-        5666 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A recombinant AAV2 plasmid encoding the ZipACR I151V mutant and soma
            targeting with KV2.1 motif..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Mermet-Joret N, Moreno A, Zbela A, Ellendersen BE, Krauth N, von 
            Philipsborn A, Piriz J, Lin JY, Nabavi S
  TITLE     Dual-color optical activation and suppression of neurons with high 
            temporal precision
  JOURNAL   eLife 2023 12:RP90327
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 2023 
            12:RP90327"
COMMENT     Sequence Label: AAV-hSyn-ZipT-mScarlet-KV2.1
FEATURES             Location/Qualifiers
     source          1..5666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(175..763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(255..274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(934..1725,1726..1794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1557..1576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1795..1899)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1925..2380)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2062..2083)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2274..2293
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2452..2581
                     /label=AAV2 ITR
     promoter        2663..3110
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4073..4768
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4206..4224)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4474..4493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5028..5048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   5475..5604
                     /label=AAV2 ITR
ORIGIN
        1 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
       61 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1921 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     1981 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2041 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     2101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     2161 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     2221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     2281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     2341 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     2401 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct
     2461 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
     2521 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     2581 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
     2641 tctgcagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg aggcggggtg
     2701 ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc
     2761 aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg
     2821 cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc
     2881 gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc
     2941 ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc
     3001 gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc
     3061 ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag tcgaattcaa
     3121 gcttggatcc atggccgccc tccatcaggc tctggaggag ctgggacagg gagccagcag
     3181 gcacctcctg tacagagccc ccgaggagat ggtgtatggc aagtgggtgg aggccaaccc
     3241 taggtgcatc gaactgcacg accactccga ctacgtgtac gtgttccagc tgtgcttcgc
     3301 cgtggtgtgc gcctgccagg tgatcttcat gttcaccagg gcccccaacg tgggctggga
     3361 agccatctat ctgcccctgg ccgaggtcgt gacctacagc attgccgcca acggagaggg
     3421 cgtgctgagg atggccgacg gcaggtactt caatttcgcc aaactggccg gatgggccgt
     3481 gtgctgcccc attatgctca tccagatcgg aggcatggcc cagatcaagt acaggaccat
     3541 ccccctgaac aacgtggtgc tggccgctag cctgaatagg accatcttcg gcatggccag
     3601 cgccatcaca gcctccgacc ctgctagatg gggcttttac ttctgcgcct ggatctgtta
     3661 cctgaccgag gtcggcatca ccctgaccat catggccgtg gccatcagcg acttcagcaa
     3721 gatcaagaca gagctcggcc agtgggtggt gggccgtatt caaaccatga ggatcatctt
     3781 tctggtcgcc tggaccagct tccccgtcgt gtgggtgctg ggctacaccg gattctgcgt
     3841 gatccacgag gactacatcg ccctgctgta tctgttcgcc gacctgctct ccaagaacac
     3901 ctggggagtg atgatgtggc ataccacctg ggtgaaactc aacggcaaat gggacaggga
     3961 atttgccgcc gccggaggac acgaggctct gaagaaggcc ctggagcagg acgtggaaat
     4021 cggcgccggc gagaagaacc agaaccagct gaccgccagg caggcggccg caatggtgag
     4081 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     4141 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     4201 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     4261 ccctcagttc atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta
     4321 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     4381 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     4441 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     4501 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     4561 gatggccctg cgcctgaagg acggcgggcg ctacctggcg gacttcaaga ccacctacaa
     4621 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtg gaccgcaagt tggacatcac
     4681 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     4741 caccggcggc atggacgagc tgtacaagca gtcccaaccc atactgaaca cgaaggaaat
     4801 ggctcctcaa agtaaaccgc ccgaagaact cgaaatgagt tcaatgccat cacctgtggc
     4861 tccgcttccc gcccgcacgg aaggtgtcat cgacatgcga agcatgtcta gtattgactc
     4921 atttattagc tgcgccaccg atttccctga agctactcgg ttctaatcta gaataatcaa
     4981 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5041 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5101 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
     5161 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5221 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatcactag ttaggcttta
     5281 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5341 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     5401 tttaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt
     5461 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     5521 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     5581 ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5641 ggtttgcgta ttgggcgctc ttccgc
//
