LOCUS       sequence-442625-        6124 bp    DNA     circular SYN 15-APR-2025
DEFINITION  srb-6 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY123
FEATURES             Location/Qualifiers
     source          1..6124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1799..1819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1844..1864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1886..1906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3715..3737)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3837..3856
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3934..3952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4020..4124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4125..4193,4194..4985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4343..4362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5156..5744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5645..5664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6032..6053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6068..6073,6074..6091,6092..6098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6106..6122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6111..6124,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ctagtggctt
      181 ctggtattgt gctctttata cttgaaagag cacaacacac ctgtgaccag agtcctcatg
      241 acaagaattt tttttcagta tttgtatatt tcgatgagaa aaaaacacac tttttgtatt
      301 tttagtttgg tcagatcttt gccaaaactg ctgaactttt gagcaaaact atttgacgca
      361 tgactttcat tctttgcgat tgatgtgcta tgcagatttt cttgaaactt tagcataccg
      421 aaattttatt aattaaaagc aattcggtct tttcgaaata atttttttaa acctcgatta
      481 atatatatta cttccttttc tccccctttt tatgtatgtt ttatgattgt gatttccaat
      541 acgtgtacat tcttgattaa tttattctct ccagcaacaa gttgcaaatt ggaattagag
      601 agtcagtgtt agagaaatac gagtttccct ctttttaatg taaagtttat tgtttcacca
      661 ctcgtgcagg tgttaacatg ctttttcgat cgcccttacg aactttttta tttatatgtc
      721 aataaatatt ttactgattt cataaaatta tttttaatca tttatttact attttaaaaa
      781 aaattaaaat gttgcatgat tttaataacc aaaattacta attttaaaaa gaaacaacac
      841 tggttttatg ttgtctgatc tgagaaattc gttagtgaaa tacaagaaaa atagcaaaaa
      901 taactatgta tgtacaaaat aatagggcac caacaaaaat atttttctgt aatatatttg
      961 tcatggtttt ctggatttta aaacactcaa aatattatct tgaatgagca aggcatagct
     1021 taatctagag aaaatgatgt gagctgatgt gaacagaaaa aagatacgtt ccaaaaagtc
     1081 accaattttc attgtattat ttaccagcat taattttttt aaatcaaggt agagtagcgt
     1141 cagctaggaa attgttaaaa cctggataaa attgccagtt attataaaaa gcatttcaaa
     1201 aataatttta aattttctaa tagtagtcaa aaattggtgg ttattcagtt ttgataattc
     1261 gtaatttagg aaaactaccg tttttttttt caaaatttta caagaagctt ttgactagaa
     1321 attttaaaat aactgaaatg ttttggaggg aatcagtatg aaatttggta tttttggatt
     1381 attttatatc tatttagata aaattggtgc cgtttctttg actatttgtt gttcaaaaga
     1441 ccagcagacc aaaagctaag cttaaaattt ctgcatatat ttcagttctt ttttacatcg
     1501 taaaagaaaa aattctaatc tttttacctc cattgcgaca ttttcccaga ctttatgcat
     1561 caaacatacc tccttttttg ttttctagaa actactgaac cggatatatg ccagttttta
     1621 ttttgtgatc agtcttgaaa tttctacaga agaaataaaa cccgggattg gccaaaggac
     1681 ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg
     1741 agggtaccgg ttttcccagt cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc
     1801 aaagaagaaa cgcaaagtac cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa
     1861 agtaccggtc gccgagaaga cagccccaaa aaagaagcgt aaagttccac gcgtcatggg
     1921 agtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agcgcgctgt
     1981 ctccaaggga gaggagctca tcaaggagaa catgcacacc aagctctaca tggagggaac
     2041 cgtcaacaac caccacttca agtgcaccca cgaaggggag ggcaagccct acgagggcac
     2101 ccagaccaac aggattaagg tggtggaggg aggccccctg ccgttcgcat tcgacatcct
     2161 ggccacctgc tttatgtacg ggagcaagac cttcatcaac cacacccaag gaatcccaga
     2221 cttcttcaag caatccttcg taagtttaaa catgatttta ctaactaact aatctgattt
     2281 aaattttcag cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     2341 gcttaccgtt acccaggaca ccagcctcca ggacggctgc ttgatctaca acgtcaagct
     2401 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     2461 ggccagtacc gagaccctgt accccgctga cggcggcctg gaaggcagat gcgacatggc
     2521 cctgaagctc gtgggcgggg gccacctgca ctgcaacctg aagaccacat acagatccaa
     2581 gaaacccgct aagaacctca agatgccagg agtaagttta aacatatata tactaactaa
     2641 ccctgattat ttaaattttc aggtctactt tgtggaccgc agactggaaa gaatcaagga
     2701 ggccgacaat gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     2761 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagt aagcgccggt
     2821 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     2881 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     2941 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     3001 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     3061 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     3121 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     3181 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     3241 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     3301 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     3361 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     3421 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     3481 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     3541 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     3601 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac
     3661 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3721 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3781 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3841 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     3901 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     3961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5881 gccgaacgac cgagcgcagc gagtcagtga gcgatgaagc ggaagagcgc ccaatacgca
     5941 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6001 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6061 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6121 aatt
//
