LOCUS       sequence-442757-        9844 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAV expression of hM4Di from RAM promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  TITLE     Nagai Lab plasmids for expression in astrocytes and neurons
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAM-d2TTA-TRE-hM4Di-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..137
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        157..471
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    165..183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    236..254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    272..290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    343..361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    379..397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     444..468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1940..1949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1946..2653
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2091..2109)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2359..2378
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2749..3337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2802..2822)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3220..3231)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3370..3387)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3376..3487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3635..3683
                     /note="synthetic polyadenylation signal"
     misc_feature    3697..3788
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3737..3756
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             join(4154..4771,4772..4777,4778..4813,4814..4816,4817..4852,
                     4853..4855,4856..4891,4892..4900)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4913..4929)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4954..4973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4955..4973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4999..5133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5162..5302
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5162..5291
                     /label=AAV2 ITR
     polyA_signal    5469..5603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5632..5797
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5981..6435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6068..6087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6277..6298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6461..6565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6566..6634,6635..7426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6784..6803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7597..8185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8086..8105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  8490..8714
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8781..9314)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9484..9489,9490..9506,9507..9512))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttgcatgcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
       61 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      121 catcactagg ggttcctgcg gccgcacgcg cgtctcgagt ttactcccta tcagtgatag
      181 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
      241 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      301 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
      361 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
      421 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc caccggtgtt
      481 tgccaccatg gccaacttca cacctgtcaa tggcagctcg ggcaatcagt ccgtgcgcct
      541 ggtcacgtca tcatcccaca atcgctatga gacggtggaa atggtcttca ttgccacagt
      601 gacaggctcc ctgagcctgg tgactgtcgt gggcaacatc ctggtgatgc tgtccatcaa
      661 ggtcaacagg cagctgcaga cagtcaacaa ctacttcctc ttcagcctgg cgtgtgctga
      721 tctcatcata ggcgccttct ccatgaacct ctacaccgtg tacatcatca agggctactg
      781 gcccctgggc gccgtggtct gcgacctgtg gctggccctg gactgcgtgg tgagcaacgc
      841 ctccgtcatg aaccttctca tcatcagctt tgaccgctac ttctgcgtca ccaagcctct
      901 cacctaccct gcccggcgca ccaccaagat ggcaggcctc atgattgctg ctgcctgggt
      961 actgtccttc gtgctctggg cgcctgccat cttgttctgg cagtttgtgg tgggtaagcg
     1021 gacggtgccc gacaaccagt gcttcatcca gttcctgtcc aacccagcag tgacctttgg
     1081 cacagccatt gctggcttct acctgcctgt ggtcatcatg acggtgctgt acatccacat
     1141 ctccctggcc agtcgcagcc gagtccacaa gcaccggccc gagggcccga aggagaagaa
     1201 agccaagacg ctggccttcc tcaagagccc actaatgaag cagagcgtca agaagccccc
     1261 gcccggggag gccgcccggg aggagctgcg caatggcaag ctggaggagg cccccccgcc
     1321 agcgctgcca ccgccaccgc gccccgtggc tgataaggac acttccaatg agtccagctc
     1381 aggcagtgcc acccagaaca ccaaggaacg cccagccaca gagctgtcca ccacagaggc
     1441 caccacgccc gccatgcccg cccctcccct gcagccgcgg gccctcaacc cagcctccag
     1501 atggtccaag atccagattg tgacgaagca gacaggcaat gagtgtgtga cagccattga
     1561 gattgtgcct gccacgccgg ctggcatgcg ccctgcggcc aacgtggccc gcaagttcgc
     1621 cagcatcgct cgcaaccagg tgcgcaagaa gcggcagatg gcggcccggg agcgcaaagt
     1681 gacacgaacg atctttgcca ttctgctggc cttcatcctc acctggacgc cctacaacgt
     1741 catggtcctg gtgaacacct tctgccagag ctgcatccct gacacggtgt ggtccattgg
     1801 ctactggctc tgctacgtca acagcaccat caaccctgcc tgctatgctc tgtgcaacgc
     1861 cacctttaaa aagaccttcc ggcacctgct gctgtgccag tatcggaaca tcggcactgc
     1921 caggcgggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
     1981 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     2041 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2101 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     2161 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     2221 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2281 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2341 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2401 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2461 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     2521 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     2581 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     2641 cgagctgtac aagtctagag tcgacccggg cggccgcttc gagcagacat gaaaacgcta
     2701 gcggcgcgcc agcgatccac cggatctaga taactgatcc ttatcgataa tcaacctctg
     2761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3121 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3181 acctggattc tgcgcgggac gcccttctgc tacgtccctt cggccctcaa tccagcggac
     3241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3301 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg actagagctc
     3361 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3421 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3481 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3541 gcaaggggga ggattgggaa gacaatagca gactagtacg tctctcaagg ataagtaagt
     3601 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     3661 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     3721 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     3781 atttctctgg cctaactggc cgacgcgtac cctagaagtt tgttcgtgac tcagactaga
     3841 agtttgttcg tgactcagac tagaagtttg ttcgtgactc agactagaag tttgttcgtg
     3901 actcagaact cattcataaa acgcttgtta taaaagcagt ggctgcggcg cctcgtactc
     3961 caaccgcatc tgcagcgagc aactgagaag ccaagactga gccggcggcc ggatccagct
     4021 catgagccat ggcttcccgc cggaggtgga ggagcaggat gatggcacgc tgcccatgtc
     4081 ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa
     4141 tgtggaattc accatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     4201 actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     4261 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc
     4321 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     4381 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
     4441 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     4501 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     4561 cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga
     4621 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     4681 gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     4741 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
     4801 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     4861 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagc atcgataccg
     4921 tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4981 agcggccgac catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5101 aaactcatca atgtatctta tcatgtctgg atctccggac acgtgcggac cgagcggccg
     5161 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     5221 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     5281 cgagcgcgca gctgcctgca ggtctgagac aataaccctg ataaatgctt caataatgta
     5341 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     5401 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5461 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5581 atgtatctta tcatgtctgg atctgatcac tgatatcgcc taggagatcc gaaccagata
     5641 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     5701 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     5761 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
     5821 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt
     5881 tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac
     5941 tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6241 tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc caaactggaa
     6301 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6361 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6421 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6481 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6541 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6601 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6661 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6721 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6781 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6841 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6901 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6961 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7021 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7081 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7141 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7201 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7261 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7321 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7381 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8341 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8401 gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga
     8461 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
     8521 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
     8581 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     8641 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
     8701 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     8761 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     8821 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     8881 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     8941 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     9001 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     9061 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     9121 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     9181 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     9241 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     9301 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     9361 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     9421 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     9481 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     9541 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     9601 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     9661 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     9721 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     9781 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     9841 cagc
//
