LOCUS       sequence-44293-e         850 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, 
            Tucker P, Ingles CJ, Blencowe BJ
  TITLE     Role for PSF in mediating transcriptional activator-dependent 
            stimulation of pre-mRNA processing in vivo.
  JOURNAL   Mol Cell Biol. 2005 Aug;25(15):6734-46.
  PUBMED    16024807
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-08"; volume: "25(15)"; pages: "6734-46"
COMMENT     Sequence Label: Myc-PSF-Delta+/-
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(6..24)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(45..61)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(45..61)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(58..80)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    69..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(93..99,100..117,118..123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    138..159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(276..293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(527..546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgttccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
       61 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      841 gctacagagt
//
