LOCUS       sequence-442960-        8601 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RADARSv3 driving mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, 
            Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
  TITLE     Programmable eukaryotic protein synthesis with RNA sensors by 
            harnessing ADAR.
  JOURNAL   Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5.
  PUBMED    36302988
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5."
COMMENT     Sequence Label: pKJ276
FEATURES             Location/Qualifiers
     source          1..8601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     418..437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     671..688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    805..826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(841..846,847..864,865..871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    879..895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     884..906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     903..919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     903..919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    933..981
                     /note="synthetic polyadenylation signal"
     CDS             complement(join(982..1695,1696..1698,1699..1701))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     1635..1656
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1698..1707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1726..1745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1727..1745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1790..1993)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1819..1839)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1994..2373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2487..3665
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2718..3656
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3613..3633
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3678..3687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3684..4391
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3829..3847)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4097..4116
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4392..4445
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_RNA        4523..4541
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             4698..4754
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5481..6048
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5498..6050
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5642..5659)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5869..5888
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6062..6661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6062..6081)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6558..6578
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6670..6894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6904..6921)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6904..6920)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6913..6935)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7129..7148)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7248..7270
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7308..7326)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7394..7498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7499..7567,7568..8359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSGGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7717..7736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8530..8601,1..517)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
       61 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      121 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      181 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      241 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      301 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      361 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      421 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      481 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      541 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      601 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      661 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      721 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      781 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      841 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      901 cacaggaaac agctatgacc atgattacgc cacacacaaa aaaccaacac acagatgtaa
      961 tgaaaataaa gatattttat tctacttgta cagctcgtcc atgccgagag tgatcccggc
     1021 ggcggtgcgg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     1081 cacggactgg gtgctcaggt agtggctgtc gggcagcagc acggggccgt cgccgatggg
     1141 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctccacgt tgtggcggat
     1201 cttgaagttg gccttgatgc cgttcttctg cttgacggcc atgatataga tgttgtggct
     1261 gttgaagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgacgcc
     1321 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     1381 gtaggtgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     1441 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaaca cctgcacgcc
     1501 gtggctcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tagtactgat
     1561 gaacttcagg gtcagcttgc cgttggtggc atcgccctcg ccctcgcccc tcacgctgaa
     1621 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     1681 ctcctcgccc ttgctcacca tggtggcggt accaagcttg cgtctcccta tagtgagtcg
     1741 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
     1801 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     1861 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     1921 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     1981 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     2041 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     2101 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     2161 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     2221 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     2281 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     2341 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcga
     2401 agctgatgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     2461 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     2521 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2581 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2641 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2701 ttgccgcgag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2761 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2821 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2881 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2941 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     3001 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     3061 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3121 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3181 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3241 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3301 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3361 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3421 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3481 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3541 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3601 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3661 cgtgacgtac gggatccgcc accatggtga gcaagggcga ggaggataac atggccatca
     3721 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     3781 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     3841 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     3901 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     3961 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     4021 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     4081 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     4141 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     4201 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     4261 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     4321 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     4381 agctgtacaa ggagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg
     4441 gcccctctag aactccaaaa gaccagtggt ggttttcgac gcaggaccac cgcgtcggca
     4501 agtctcctca ttggatccag atacatgagg atcacccatg tgcatccatc tttttcagcc
     4561 atctttagaa ggttcaggtt gttttctgcc aggacgcagg accaccgcgt cctttgctgc
     4621 tttcacacag gttactcaca tggggatcac ccaggtacag gtctcctttc tcagggctga
     4681 gagctagcgg aagcggagct actaacttca gcctgctgaa gcaggctggc gacgtggagg
     4741 agaaccctgg acctgtaagt aaggtaagta aggtcagcaa aggcgaagaa gacaacatgg
     4801 catcactgcc agcgacacac gaacttcata ttttcggctc catcaatggt gtggacttcg
     4861 atatggttgg acagggtact gggaacccta acgatggata tgaggagttg aacttgaaga
     4921 gtaccaaggg cgatcttcag ttttcacctt ggattctcgt acctcatata ggttatggct
     4981 ttcatcaata tctgccctat cctgacggta tgtcaccttt ccaagcagct atggttgacg
     5041 ggagcggtta tcaggttcac cggacaatgc agtttgaaga tggagcctct ttgaccgtaa
     5101 attatcggta tacttatgag ggcagccata ttaaaggcga ggctcaagtt aaggggactg
     5161 ggttccccgc ggatggtccc gtaatgacaa acagtctgac ggctgcagac tggtgccgct
     5221 ctaagaagac atatccaaat gacaagacga ttattagcac tttcaagtgg tcatacacta
     5281 caggaaatgg taaacgctat aggagcactg cccggaccac gtatactttc gcgaagccga
     5341 tggcggcaaa ttatttgaaa aaccaaccga tgtatgtgtt cagaaagaca gaactcaagc
     5401 attctaaaac tgaactgaac ttcaaagagt ggcagaaagc ctttactgat gtaatgggca
     5461 tggacgaact ctataagtaa tccctccccc ccccctaacg ttactggccg aagccgcttg
     5521 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5581 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5641 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5701 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5761 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5821 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5881 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5941 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6001 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     6061 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta
     6661 agcggccgcc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6721 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6781 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6841 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggctcgaa
     6901 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6961 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7021 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     7081 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     7141 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     7201 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7261 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     7321 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     7381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctgg
     8221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8581 accgctacca gcggtggttt g
//
