LOCUS       sequence-442962-        9382 bp    DNA     circular SYN 15-APR-2025
DEFINITION  RADARSv3 driving luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, 
            Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
  TITLE     Programmable eukaryotic protein synthesis with RNA sensors by 
            harnessing ADAR.
  JOURNAL   Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5.
  PUBMED    36302988
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 
            10.1038/s41587-022-01534-5."
COMMENT     Sequence Label: pKJ275
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(401..505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     573..591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(629..651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     751..770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     964..986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     978..995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     979..995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1005..1229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1238..1837)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1321..1341)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1818..1837
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    1849..2401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1851..2419
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2011..2030)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2240..2257
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(2420..2974)
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="GVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGK
                     LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ
                     GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR
                     CATFASKIQGQVDKIKGAGGD"
     CDS             complement(2984..3040)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_RNA        complement(3197..3215)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(3293..3346)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3347..4054)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3622..3641)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3891..3909
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4051..4060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4073..5251)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4082..5020
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4105..4125)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     enhancer        5365..5744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5745..5948
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5899..5919
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5993..6012
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5993..6011
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6037..7698
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     YPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     polyA_signal    7699..7747
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7761..7777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7761..7777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7774..7796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7785..7801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7809..7815,7816..7833,7834..7839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7854..7875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7992..8009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8163..8751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8243..8262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8922..9382,1..331,332..400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSGGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
       61 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
      541 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
      601 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      661 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      721 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      781 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      841 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      901 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      961 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagccatag agcccaccgc
     1021 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
     1081 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
     1141 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
     1201 aacagatggc tggcaactag aaggcacagg cggccgctta ggcaccgggc ttgcgggtca
     1261 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     1321 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     1381 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     1441 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     1501 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     1561 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     1621 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     1681 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     1741 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     1801 gggtggcgag gcgcaccgtg ggcttgtact cggtcatatg tgtggccata ttatcatcgt
     1861 gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt ttttaacctc
     1921 gactaaacac atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt
     1981 accttctggg catccttcag ccccttgttg aatacgcttg aggagagcca tttgactctt
     2041 tccacaacta tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct
     2101 tatacacgtg gcttttggcc gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg
     2161 caaagggtcg ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc
     2221 acgacattca acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc
     2281 gtcaagaaga cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg
     2341 gaaaataaca tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg
     2401 ttaggggggg ggggagggat tagtcaccac cggccccctt gatcttgtcc acctgaccct
     2461 ggatcttgct ggcaaaggtc gcacagcgtt gcggcagcca cttcttgagc aggtcagaac
     2521 actgcacgtt ggcaagccct ttgaggcagc cagttgtgca gtccacacac agatcgacct
     2581 gtgcgatgaa ctgctccatg ggctccaagt ccttgaaccc aggaatctca ggaatgtcga
     2641 cgatcgcctc gcctatgccg ccctgtgcgg actctttgtc gccttcgtag gtgtggcagc
     2701 gtcctgggat gaacttcttc atcttgggcg tgcacttgat gtgggacagg cagatcagac
     2761 agcccctggt gcagccagct ttccgggcat tggcttccat ctctttgagc acctccagcg
     2821 gcagcttctt gccgggcaac ttcccgcggt cagcatcgag atccgtggtc gcgaagttgc
     2881 tggccacggc cacgatgttg aagtcttcgt tgttctcggt gggcttggcc tcggccacag
     2941 cgatgcagat cagggcaaac agaactttga ctcccttact tacaggtcca gggttctcct
     3001 ccacgtcgcc agcctgcttc agcaggctga agttagtagc tccgcttccg ctagctctca
     3061 gccctgagaa aggagacctg tacctgggtg atccccatgt gagtaacctg tgtgaaagca
     3121 gcaaaggacg cggtggtcct gcgtcctggc agaaaacaac ctgaaccttc taaagatggc
     3181 tgaaaaagat ggatgcacat gggtgatcct catgtatctg gatccaatga ggagacttgc
     3241 cgacgcggtg gtcctgcgtc gaaaaccacc actggtcttt tggagttcta gaggggccgg
     3301 gattttcctc cacgtccccg catgttagaa gacttcccct gccctccttg tacagctcgt
     3361 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     3421 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     3481 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     3541 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     3601 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
     3661 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
     3721 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     3781 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
     3841 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
     3901 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     3961 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
     4021 tggccatgtt atcctcctcg cccttgctca ccatggtggc ggatcccgta cgtcacgaca
     4081 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
     4141 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
     4201 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
     4261 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
     4321 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
     4381 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc
     4441 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
     4501 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
     4561 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
     4621 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
     4681 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
     4741 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
     4801 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
     4861 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
     4921 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
     4981 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     5041 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     5101 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     5161 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     5221 tgcgctctgc ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg
     5281 tccattagct gcaaagattc ctctctgttt aaaactttat ccatctttgc atcagcttcg
     5341 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     5401 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5461 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5521 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5581 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     5641 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     5701 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5761 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5821 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5881 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5941 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     6001 ctcactatag ggagacgcaa gcttggtacc gccaccatga agaccttaat tcttgccgtt
     6061 gcattagtct actgcgccac tgttcattgc caggactgtc cttacgaacc tgatccacca
     6121 aacacagttc caacttcctg tgaagctaaa gaaggagaat gtattgatag cagctgtggc
     6181 acctgcacga gagacatact atcagatgga ctgtgtgaaa ataaaccagg aaaaacatgt
     6241 tgtcgaatgt gtcagtatgt aattgaatgc agagtagagg ccgcaggatg gtttagaaca
     6301 ttctatggaa agagattcca gttccaggaa cctggtacat acgtgttggg tcaaggaacc
     6361 aagggcggcg actggaaggt gtccatcacc ctggagaacc tggatggaac caagggggct
     6421 gtgctgacca agacaagact ggaagtggct ggagacatca ttgacatcgc tcaagctact
     6481 gagaatccca tcactgtaaa cggtggagct gaccctatca tcgcctaccc gtacaccatc
     6541 ggcgaggtca ccatcgctgt tgttgagatg ccaggcttca acatcaccgt cattgagttc
     6601 ttcaaactga tcgtgatcga catcctcgga ggaagatctg taagaatcgc cccagacaca
     6661 gcaaacaaag gaatgatctc tggcctctgt ggagatctta aaatgatgga agatacagac
     6721 ttcacttcag atccagaaca actcgctatt cagcctaaga tcaaccagga gtttgacggt
     6781 tgtccactct atggaaatcc tgatgacgtt gcatactgca aaggtcttct ggagccgtac
     6841 aaggacagct gccgcaaccc catcaacttc tactactaca ccatctcctg cgccttcgcc
     6901 cgctgtatgg gtggagacga gcgagcctca cacgtgctgc ttgactacag ggagacgtgc
     6961 gctgctcccg aaactagagg aacctgcgtt ttgtctggac atactttcta cgatacattt
     7021 gacaaagcaa gataccaatt ccagggtccc tgcaaggaga ttcttatggc cgccgactgt
     7081 ttctggaaca cttgggatgt gaaggtttca cacaggaatg ttgactctta cactgaagta
     7141 gagaaagtac gaatcaggaa acaatcgact gtagtagaac tcattgttga tggaaaacag
     7201 attctggttg gaggagaagc cgtgtccgtc ccgtacagct ctcagaacac ttccatctac
     7261 tggcaagatg gtgacatact gactacagcc atcctacctg aagctctggt ggtcaagttc
     7321 aacttcaagc aactgctcgt cgtacatatt agagatccat tcgatggtaa gacttgcggt
     7381 atttgcggta actacaacca ggatttcagt gatgattctt ttgatgctga aggagcctgt
     7441 gatctgaccc ccaacccacc gggatgcacc gaagaacaga aacctgaagc tgaacgactc
     7501 tgcaatagtc tcttcgccgg tcaaagtgat cttgatcaga aatgtaacgt gtgccacaag
     7561 cctgaccgtg tcgaacgatg catgtacgag tattgcctga ggggacaaca gggtttctgt
     7621 gaccacgcat gggagttcaa gaaagaatgc tacataaagc atggagacac cctagaagta
     7681 ccagatgaat gcaaataaaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     7741 ttgtgtgtgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7801 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7861 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7921 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8041 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8101 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8161 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8281 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8341 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8401 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8461 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8521 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8581 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8641 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8701 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8761 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8821 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9061 ccagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9361 ccgatcgttg tcagaagtaa gt
//
