LOCUS       sequence-443343-        5329 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Formaldehyde sensor based on the frmR repressor, which expresses 
            yeast alcohol acetyl transferase (ATF1) upon detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Dutto A, Kan A, Saraw Z, Maillard A, Zindel D, Studart AR
  TITLE     Living Porous Ceramics for Bacteria-Regulated Gas Sensing and Carbon
            Capture.
  JOURNAL   Adv Mater. 2024 Dec 10:e2412555. doi: 10.1002/adma.202412555.
  PUBMED    39659127
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Mater. 
            2024 Dec 10:e2412555. doi: 10.1002/adma.202412555."
COMMENT     Sequence Label: pFSKm4-ATF1
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      996..1218
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      3497..3568
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3584..3611
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4056..4110
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    4057..4075
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4087..4105
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4111..4129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(join(4255..5046,5047..5115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNKAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4878..4897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5116..5220)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5288..5306
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(join(5327..5329,1..948))
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
       61 tgagaacgaa ccattgagat catgcttact ttgcatgtca ctcaaaaatt ttgcctcaaa
      121 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg
      181 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
      241 gttctcaagt tcagttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
      301 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
      361 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
      421 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
      481 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
      541 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
      601 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
      661 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
      721 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
      781 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
      841 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
      901 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
      961 gtctaggtaa ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt
     1021 ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     1081 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat
     1141 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc
     1201 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca
     1261 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct
     1321 cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc
     1381 gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta
     1441 aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa
     1501 agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac
     1561 tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc
     1621 cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc
     1681 ttacccgtct tactgtcaag aggacatccg gtcaaataaa acgaaaggct cagtcgaaag
     1741 actgggcctt tcgttttgct gaggagactt agggacccta aattttattc tccagtgtta
     1801 tatactatag gggggtatgc attgacatat agcatacact cctatagtat attgcgtgca
     1861 gataacctgc aggtactaga gaaagagggg aaatactaga tgaatgaaat cgatgagaaa
     1921 aatcaggccc ccgtgcaaca agaatgcctg aaagagatga ttcagaatgg gcatgctcgg
     1981 cgtatgggat ctgttgaaga tctgtatgtt gctctcaaca gacaaaactt atatcgaaac
     2041 ttctgcacat atggagaatt gagtgattac tgtactaggg atcagctcac attagctttg
     2101 agggaaatct gcctgaaaaa tccaactctt ttacatattg ttctaccaac aagatggcca
     2161 aatcatgaaa attattatcg cagttccgaa tactattcac ggccacatcc agtgcatgat
     2221 tatatttcag tattacaaga attgaaactg agtggtgtgg ttctcaatga acaacctgag
     2281 tacagtgcag taatgaagca aatattagaa gagttcaaaa atagtaaggg ttcctatact
     2341 gcaaaaattt ttaaacttac taccactttg actattcctt actttggacc aacaggaccg
     2401 agttggcggc taatttgtct tccagaagag cacacagaaa agtggaaaaa atttatcttt
     2461 gtatctaatc attgcatgtc tgatggtcgg tcttcgatcc acttttttca tgatttaaga
     2521 gacgaattaa ataatattaa aactccacca aaaaaattag attacatttt caagtacgag
     2581 gaggattacc aattattgag gaaacttcca gaaccgatcg aaaaggtgat agactttaga
     2641 ccaccgtact tgtttattcc gaagtcactt ctttcgggtt tcatctacaa tcatttgaga
     2701 ttttcttcaa aaggtgtctg tatgagaatg gatgatgtgg aaaaaaccga tgatgttgtc
     2761 accgagatca tcaatatttc accaacagaa tttcaagcga ttaaagcaaa tattaaatca
     2821 aatatccaag gtaagtgtac tatcactccg tttttacatg tttgttggtt tgtatctctt
     2881 cataaatggg gtaaattttt caaaccattg aacttcgaat ggcttacgga tatttttatc
     2941 cccgcagatt gccgctcaca actaccagat gatgatgaaa tgagacagat gtacagatat
     3001 ggcgctaacg ttggatttat tgacttcacc ccctggataa gcgaatctga catgaatgat
     3061 aacaaagaaa atttttggcc acttattgag cactaccatg aagtaatttc ggaagcttta
     3121 agaaataaaa agcatctcca tggcttaggg ttcaatatac aaggcttcgt tcaaaaatat
     3181 gtgaacattg acaaggtaat gtgcgatcgt gccatcggga aaagacgcgg aggtacattg
     3241 ttaagcaatg taggtctgtt taatcagtta gaggagcccg atgccaaata ttctatatgc
     3301 gatttggcat ttggccaatt tcaaggatcc tggcaccaag cattttcctt gggtgtttgt
     3361 tcgactaatg taaaggggat gaatattgtt gttgcttcaa caaagaatgt tgttggtagt
     3421 caagaatctc tcgaagagct ttgctccatt tacaaagctc tccttttagg cccttaataa
     3481 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3541 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     3601 gcctttctgc gacggagcca atgtacgcaa aaaaccccgc ttcagcgggg ttttttcgcc
     3661 aaaaaaaacc ccgccctgtc aggggcgggg ttttttttta gggccctact gactgtctat
     3721 ttaagatagg ctcgaaccag ttcaatagtg tcgtcaacgg attggctgac ttcgcggctg
     3781 tagcagtcat ttcggtcaaa cgtttcccgg atatggcttt caagcacttc tgccatcagc
     3841 ccattagccg cgccccgaac ggcagcgatc tgttggagta tggcacggca ttcggcatca
     3901 ccctccagcg accgttccag agcatcaatc tgccctcgaa tacgacgaac tcgagtaagg
     3961 acctttttct tctcttccgg agtactgggc attttttttt cctccttata agggggtagg
     4021 gggtctagat ttttgtcgaa ctattcattt cacttttctc tatcactgat agggagtggt
     4081 aaaataactc tatcaatgat agagtgtcaa tctctatcac tgatagggat catctggcca
     4141 ttcgatggac ttaattaacg gcactgcgaa aaaaccccgc cgaagcgggg ttttttgcga
     4201 aatataatga ccctcttgat aacccaagag ggcatttttt aatgcccatg gcgtttacca
     4261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt tattcagctc
     4621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4861 cccagcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5221 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5281 ctataaaaat aggcgtatca cgaggccctt aggccaaata ggccgttca
//
