LOCUS       sequence-443357-       14079 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express MYRFL gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, 
            Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, 
            David J. Papke, Mateo Valenzuela, Brianna Silverman, Amanda 
            Falzetta, Scott B. Ficarro, Jarrod A. Marto, Christopher D.M. 
            Fletcher, Antonio Perez-Atayde, Thierry Alcindor, Akiko Shimamura, 
            John R. Prensner, Richard I. Gregory, Alejandro Gutierrez
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation
  JOURNAL   Molecular Cell, 2025, ISSN 1097-2765
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, 2025, ISSN 1097-2765"
COMMENT     Sequence Label: rag2:MYRFL_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..14079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1493..1513)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1493..1511)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1532..1548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1532..1548)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1545..1567)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1556..1572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1580..1586,1587..1604,1605..1610))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1625..1646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1763..1780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1934..2522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2014..2033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2693..3484,3485..3553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3316..3335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3554..3658)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3685..4140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3772..3791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3982..4003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4266..4288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4280..4297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4281..4297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4307..4325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4380..4396
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4380..4396
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(5577..5579,5580..5582,5583..6296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5577..5579,5580..5582,5583..6293)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5622..5643)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5883..5902)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6230..6251
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6598..6732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacctgctgg tttatactgg ggcattagga cccacacaga ccacaggttg acctgctggt
       61 ttgtactggg gcatttggac ccacacagac cacaggttga cctgctggtt tatactgggg
      121 cattagaacc cacacagacc acaggttgac ctgctggttt atactggtgc attaggaccc
      181 acacagacca caggtagacc tgctggttta tactggggca ttaggaccca cacagaccac
      241 aggttgacct gctggtttat actggggcat taggacccac acagaccaca ggtagacctg
      301 ctggtttact aacacctctt ccgggagcaa cctagctttc ccatgtggtc tcccttttag
      361 gtacttgccg gacacttcag ctgctggtca agaggctagc taaagctaaa atcataaata
      421 tacatttaaa acagattagt tttatataac atattaatta aaactaaatt gaactttaaa
      481 catcttttaa ttgcctgtga ttgtataata tttcacagat tgttcccatt cgttggtcgt
      541 gtctagttag cgcacatgca atcgcaacag attttccttt aagacctgca ttcgacactg
      601 aaatcacact cttggtaatg catctgtgac agtctggatg taatgtgctg ttaaaatagt
      661 ctcctcggtg tgcatctgag tggtctgtgg catcgggttc ggtgtggagc agaatgattc
      721 actctcatta caccaaatat cgaatgcgtg agtcacacat cacattttgg caggagggga
      781 agctcggatt ttgtaaaaat tctccttatt tcccgcaggc ttatatttgc attaactgtt
      841 tctgcttggc ggctctttaa aggaacgaga gaaattaggc gactgttgtt gaaagaacga
      901 gagagagagt gaagaaggga acagctgtgc cttcctcatt agcataatgt ttttgttttt
      961 cggtgggggc ttcgagccct cgtgcagttg tgtgtttgtt ttggaggccg agagatgtgt
     1021 gtgcaagagt gcaaaacaaa aacccgtaac cacgagtgcc tgaagaacag tgaaaaacac
     1081 aaacagcgga gcacctgtgt ctgaagagca gggccatgaa gagaaggctc agcgaatccc
     1141 ctgaggcgca acgcaaaata aaggattcct ccggtgtctc tgagcgcttt attctcctgc
     1201 tcaagcgcac ttacatgccg gtgttcacac ttacacccgc cgcagaacaa acctgttgag
     1261 ttctggagtg gatttgcata cggcgatggc aaatgaaggc tagttttttc tagctctgtc
     1321 gtaaaaagag acagacgata agaaactgag aatgaaatgc aaggcacaac acaacagttc
     1381 tacagtgttt gcattataat gatgaacgta tactatttta cattaggatt gattttgctc
     1441 gatagggata acagggtaat tcgagggggg gcccggtacc cagcttttgt tccctttagt
     1501 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1561 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1621 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1681 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1741 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1801 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1921 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3061 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3121 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3181 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3241 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3301 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3361 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3421 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3481 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3541 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3601 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3661 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4081 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4141 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4201 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4261 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     4321 atagggcgaa ttggagctcc accgcggtgg cggcctaggg ataacagggt aatggccgct
     4381 ctagaactag tggatctgat ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     4441 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4501 atcatgtcac atctattacc acaatgcaca gcaccttgac ctggaaatta gggaaattat
     4561 aacagtcaat cagtggaaga aaatggagga agtatgtgat tcatcagcag ctgcgagcag
     4621 cacagtccaa aatcagccac aggatcaaga gcacccgtgg ccgtatcttc gcagatctaa
     4681 agcttaaatc agttgtgtta aataagagac attcaaaata aatgtaaatg agctctccaa
     4741 atcagcagac ttaacattct ttaaaatgat tgattcaata gtgataaaaa tcaggcatag
     4801 ccagttgtaa ctttagataa attacagaaa atgtcaaata cagagaaccg attctttttt
     4861 atgatacatc caagcacaca tttaacacaa tccaggcaaa ccccgaattt cacagtcaca
     4921 agcactgttt gtacaagagc tttgcctaag gacacacagt ctctataagt ccaggtcgtt
     4981 ggtttcactc ttattttaaa catgtgacat ttttcctgcc atcctgtctt aggctgctgt
     5041 ttgcttcatt ccatgtcaca ttaaattcct cagtagcacc ttttacacac acagccaatc
     5101 ttttccagaa aattcaattg ctttgaagag ataatgtgtg aacaaatcca tttagaaaag
     5161 gaaaattaag aatttgtaaa atcatctgta aattgttggc attcttctgt atatgaacat
     5221 cacatcattt acaggtaaag gtctggtcat taattatatg acaatttact ggtattattt
     5281 tgtgaaaggg gctattttca atgcgttcat ccatcctttt catccctcaa atctctcatt
     5341 cacgtccccc tccccatctg cacactttat ctcatttcca ccctgctgga atctgagcac
     5401 ttgtgcagtt atcagggctc ctgtatttag gaggctctgg gtgtccatgt aggggacgaa
     5461 cagaaacact gcagaccttt atagaagaac aattgataag agtcctcata cataaagact
     5521 ccattagtaa gccagtgacc caggagccca gaccaacagc aaagcagaca gtgaccatgg
     5581 tgagcaaggg cgaagagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5641 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5701 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5761 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5821 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5881 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5941 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     6001 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     6061 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6121 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6181 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6241 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     6301 gcccctctcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag
     6361 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6421 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagagat
     6481 ctgatctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     6541 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     6601 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6661 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6721 catgtctgga tcatcatcga tttaggcaca gcgtcgatag aagtaaaaga agtaatcggt
     6781 gaagaatatc ccagcaaaat aaggatcagt tgaacagtca gctaaatccg gtgcagctac
     6841 acggaaatgg aacatgctgt cagaaaatgg ggctacaggg aggctccaaa tgtgctgata
     6901 tccctgtgtc atctcctggg agatttctct gtggctttcc agccctttgg ttcccctttt
     6961 actatggaaa catatatttc caagggtgaa tttgcactgg aagactatca atggctctgt
     7021 tgtgtttatt tccagagaga acttgacatt ggtgggtgtg tgtttattaa caggaatatt
     7081 gtaattataa tttccagatc cgcactggag gctttgaatg caatactgat gatctattat
     7141 ttgctggatt tccataatct gaatagaact gatggttgta tcaatccagt cactctccca
     7201 gtcagggccg ctgagtgcat ttcttgccaa aactgatttg cttttgtctt ctgaccatcg
     7261 cctctgatgg aattccttct cctcagattg tctttgcaca ggacttgaag agacttcttt
     7321 cattccccgg atggggcagc aataagtctc ctgacacggc aggatttcac agaaagtaat
     7381 ttcaggtact tgtaaggagg ccggtgtggt taccagggat gtattaggtg ctgaggagga
     7441 ggctgtgggc agcagagctg gctcctgtga gcttgtgata ttgcttggag ggagatttgg
     7501 gacacgtcgg tcttgatctt ttaagctaag tatatagagg gcaactatcg tcaaagcaca
     7561 aaatgccatc acagcaatca gagttataac caaggtctgg aaaacccaat taggacacgc
     7621 ctgtcttttt cctgaaaaat aaaccttgtt gtttttttta tgaacggccc ttcttggaga
     7681 agatgcacta acggctctgc tagatttgct gattgtgctt gcttcactgg ctgatgactt
     7741 ccaactactg agccgcttta gccgggccag ctttctgttc catatttcta actcttctat
     7801 tctttcctca aggttgttag ttagtttgca cagttgcttc actgcaccta cattttccat
     7861 aaagatctgg tccttatcca ccatgaggaa gttctccaag gtctctccgt ttccgcaggt
     7921 gacatcacca acctctctta ctgctctggg caggatttct tgcacctcct gggcaatcat
     7981 tcctgtttga tgggcagtgt ttattcccat tgcagatgca aattcaggtt tgtagtcata
     8041 ttcaacaatt ctcatttggg ctattctttt cagctgttca ttcgtgtcaa cctcctggat
     8101 attctgcttt gcccggctgt cagagggatg catgatggtc cccatcactt tcatgttgcc
     8161 acagacaacc agggcttcgt ccggcgcatc tgtgttgatt cctactcgac cgtgacagac
     8221 aatagattct ggaacttgtc ctcgctgcca caatgcatca ctgtcatttt caaactgccc
     8281 agggttagag gcccttacaa tgatcctttc agagatgtgg gcagacaaca gatagaactg
     8341 gtcttggtta gcagcataca gtccaaccac caacatgaag tatctctggt ctggatttgg
     8401 ttttcccttt tttctcatat tatttgctgt ggtttcgctg aagtgtaatc gtcccagtgt
     8461 tactttggtg acctggtcag ccagtaggtc aattttaaca ggattgaaaa tctttttgct
     8521 cctatctgct tgggactgtt caatagcaat tatttgattg gtagcttcca ccttagtgcc
     8581 aaaaactttc aagtaaaaca tttctattgg ctttaggccc atctcggttt caacaaattt
     8641 tggacttccc caaacttgga tgtgaatggt tatctggaaa tgattcttct tttggcaaac
     8701 aaaagcttca tctgctggtg aaaaattaaa tcctttgtct gttacaactc gatagcccac
     8761 atcaggtagt ttttcataat gactgtttaa taagctgtgc caaggaacac tatggcatgg
     8821 ttgccacttc agagcaggag agcactggtc tgtaggcatt ctgttctgtc cctcactgtc
     8881 agggtcctgg acttcactgc tgcgactcct acttgtcatc ggtctgcagt ggcaggccca
     8941 cactcggcat tccccggagt cctccagtgc ctgcgtgcac tttctcttct tggttggagg
     9001 caatgaggct ccagggctgt gacacagagg ctgctgaggg taagaacaac ctatccccag
     9061 atgggaggac acggattggt ccaggggggt ggcaagatga ctggcgtttg agtggcagct
     9121 gtgaaagcct ccgtggattt ggcaggagtc gcccattcca gaggtgctct gcagcgggtg
     9181 catgggcggc atcgcaggag cagctgtggg gtggagaaaa ggagctggag tcctcccagc
     9241 tgtgggcctc agggttgggt agcatgcacc tttgacctgc ggaggagagc atgagtcgga
     9301 tgcagaatag ggcggggtgt ctgggagctg gcgttgcaag gcccccaaat caaagtcatt
     9361 gcccagaaat tcctccaaca gacttgtgtc cagggctggg ttctccagag taccattggc
     9421 tccctgagct tcaaagaact gctgcagggc ctcattttcg cctaccacat ccatggatcc
     9481 ctttttgaag gtagctgtgt aaaaaaaata aaaaaaaaca ttaatttata gtcatttaat
     9541 tgtacaatgt taattttatt acctcttata aaaaccaacc atggctttac cacagataat
     9601 acttaaactt aaagataaca atggcaacca tagtgttaca ctacactaga catagtttaa
     9661 ccatagtatt tgtagtataa ctgtgatgtt taaatgatta tcattgtatc aaagaatgct
     9721 agtgcttcaa attagcataa gagtaagcga agtgtgtagt taggtcttat tttattattc
     9781 aaagtaagaa aaaaacgcgg aaccatatct ttctttcgct gtttccccga aaacagaaac
     9841 attttccaca ggaaattgta gttttcctaa ctctagtttc ccgttgagac gcacattaag
     9901 catacaatat aaggactaca gtacccagca ttcagtgcga gagcggcgtc tgcgtttaaa
     9961 cggtaggctt tatctgatgt acttttctat gtgctccaaa agctactatt ctcgtatttt
    10021 cttcaacatt tttctgccgt ttctacatct gatatgtttt agattcgcct gaaggtaatc
    10081 aaatacgcaa gagaagcatt aacaaacaaa ttagcaagca ctaataataa ctgtcaacac
    10141 gagtaactga gcactacatt aacacagtaa cgttatatgt aaaccacgtt acctactgta
    10201 gcctatattt taatatatat cattttacat tatgcaaatc gtatttccat cagttcctct
    10261 actctatatg tcacttaaaa cacgtacgct tccaaatatt acatatctag tcttacaata
    10321 gtcgtcctag taatgtaacg ttacttctta cctcttggtc agtgtactcc gaaatctgag
    10381 agctgtttgg tgctggaaat ttttatttta gatattaacg cgaattgttt agtgagtgag
    10441 ccagaggttg agtgtttgtg aaggttgact ggctcctctt gaggtttact gtttgcaact
    10501 gctgctcaca acaggtgttt gagcacaatc acacgagttt gaatgaaaac atgcacaaac
    10561 tcactgcatc attggctgtt tattaatatg gagttctggt tttgtgctgc tgatcagcaa
    10621 tactcataga tattatattt ttccgtgcaa ttttagtcat tatgaatgtt tccaggtctt
    10681 tccaaatggg gacataaccc aaccaccccc cgcgcttgat acggttctgt atatgcatat
    10741 attcacagta atacttttgt atagtaacca atagcatagg ttaatataca aaaaaaaaaa
    10801 aaaaatccaa attgataata atatacattt ttagcaatgt gttaatattt tctgaaggat
    10861 catgtgacac taaaattcag attgaataca atttagcttt ggcattaaag aaataacatt
    10921 gtcaaaaata tttaaaatat aatatagtta ttttaaatgg aaacaatgta tcccgatatt
    10981 gctttttcga ctgtaattgt gatcaaataa atgcagactt ggtgacataa agtttttttt
    11041 ttaaatagtg ctgtaaatta tttggattat ctagatatcc ttaacaatat acactcaccg
    11101 gccactttat tatgtacacc ccactagtac tgggttggac ctctttttgc cttagcatag
    11161 attcccttcc ttttgcattt caaccaggta ctggaaaatt cctcagagat tttggtccat
    11221 attgacatgc tagcatcatg cagatttgtc ggctgcacat ccaggatgcg aatcttccat
    11281 tctaccacac cccaaaggcg ctctattgga ttgagatctg gtgactgtgg aggtcattcg
    11341 agcacagtga actcattgtc atgttcaaga aaccagtctg agatcattca cgctttatga
    11401 catggtgcat tatcctgcag gaagtagcca tcagaagatg ggtacactgt ggtcaaaaag
    11461 aaatgaacat ggtcagcaac aatactcagt taggctgtgg cgttgacacg atggttaatt
    11521 ggtactaatg ggcccaaagt gtgccaagaa tatatccccc acaccattac accaccatca
    11581 ccagcctgaa ctgttgatac aaggtagaat gtatccatct gaccaggcaa cataatgttc
    11641 aatcttctat tgtctactta tgttgagcct gtgcgaattg tagcctcagt ttcctgttct
    11701 tagctgacag gagtggcacc cgatgtggtc ttgagctgct gtagcccacc cgcctcaagg
    11761 ttcgacatgt tgtgttcaaa ggtgctcttc tgcatgcctc agttctaacg agtggttatt
    11821 tgaattactg ttgcctttct atcagctgga accagtcagg ccattctcct ctgacctctg
    11881 gcatcaacaa gacatttgcc cccacagaac tgccgctcac tggatattat ctttttttgg
    11941 accattctct gtaaacccca gagatgtttg tgtgtgaaaa ccccagtaaa tcagcagttt
    12001 ctgaaatact cagaccagcc cgtctggcac caacaaacac gtcacgttta aagtcactta
    12061 aatcaccttg cttctccatt ctgatgctcg ctttgaactg cagcagatcg tcttgaccat
    12121 gtctacatgc ctaaatgcac tgagttgctg cctggtgatt ggctgattag aaatttacgt
    12181 taacaagcag ttagacaggt gtacctaata aagtggccgg tgagtatttt tcagattttt
    12241 ttttttcaaa ttttaaatag tgaaatcata ttattatcag acaatcacca tcaaagtaca
    12301 acactgttta caggcaatta aattaaacta attctgcttt gaagtcgtat ttacatgcaa
    12361 tttgagactg aatattcaaa gtagcgtaat aaacaatata aagagcatca caagttgtca
    12421 ctgattaaaa ataaccaaat gtgtgaataa taaaacaatt ctacctcaat ctttaaaatt
    12481 catatatttt attaatcatg catttattta cattttacgt aaacattttc atacatttat
    12541 tttcttttcg gcttagtctc tttattaatc tggggttaaa cgcacgcgaa tgctccacac
    12601 agaaacatca agtgatccag ctgaggctcg aaccagcgac cttcttgctt tgctggcgac
    12661 agcactacct actgcaccac cgtgtcgttg taaacattat tttgtataat ttatttaaaa
    12721 caatttaatt attttaaatt acgtttgttt ttagtttttt atactaatgc tgcttatctt
    12781 gtttatcaat tttactataa ttttcaacaa tgaaattatt aattatttta gttttatttt
    12841 tacttataaa attttttttt ttcccccaaa catttcatta gccctatagc acttccttgc
    12901 agctccctga ggtaccccag ggcaaagttt taatggcccc tagctctggc acaagttatc
    12961 cagaaagttc cattataata agctaatgat aattccttac atttatatcg cgcttttctg
    13021 gaaactcaaa acactttttt gggcaaaatc tcctcatcaa ccaccagtgt gcagcatcca
    13081 cctggatgac gcgatggcag ccatattgca ccagaccgca cactacacac cagtatattg
    13141 gtggagagga gacaggggct gcgtccgaaa ccgtctacta ctacttagta ggtactgtat
    13201 ttgaatttaa acttactact tggctgtggg aaaagaacat tctataaaat atgaatgtga
    13261 gcagtatgaa tggaacttgg acgtactaca tccgccaata tgtcatcgtc acgtaaccta
    13321 cccgcatgag ttgcgttgct tctctccgat tcaagaattc tctcacgggg catcatggaa
    13381 tagcgtagcg tgcatcgaat gcacactcta gaatctcgcc ggaagtattc ggacatatta
    13441 cttggctcgc atactgattt tagcgtacct cataaaatgg aagtatgcga tttcggcccc
    13501 agctagagtg ataaagccaa ttatgatatg gggatggtta ggaggccatg atggacagag
    13561 gacagtgagc aaatttgtcc aggatgccag ggtaggaagg acatcctggg aattttttac
    13621 caccacagag agtcgggacc ttgttttaac atcttatccg aaagatggag ctcaccgagc
    13681 agtatagagt ctccatcaat atactggggc attaggaccc acacagacca caggttgacc
    13741 tgctggttta tactggggca ttaggaccca cacagaccac aggttgacct gctggtttat
    13801 cctggggcat taggacccac acagaccaca ggttgatctg ctggtttata ccggggcatt
    13861 aggacccaca cagaccacag gttgacatgc tggtttatcc tgggacttta ggacccacac
    13921 agaccacagg ttgatctgct ggtttatact ggggcattag gacccacaca gaccacaggt
    13981 tgatctgctg gtttataccg gggcattagg acccacacag accacaggtt gacatgctgg
    14041 tttgtaccgg ggcattagga cccacacaga ccacaggtt
//
