LOCUS       sequence-443590-       10441 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) 
            in a pBIG1a vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10441)
  TITLE     Baculovirus expression plasmids for production of recombinant 
            Strep-tagged HOOK3 bound to FTS and FHIP1B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBIG1a_StrepII-Hook3_FTS_FHIP1B
FEATURES             Location/Qualifiers
     source          1..10441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2103..2122)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2147..2195
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2290..2381
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2434..2457
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2494..2517
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2518..2541
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    4855..4989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5145..5236
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6321..6455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6612..6703
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9828..9962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  10276..10441
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg ggtttaaacg ctctatggtc taaagttttt atcgaggcta
     2101 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     2161 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     2221 ctggcactct gtcaagtgaa cgctctatgg tctaaagatt taaatcgacc tactccggaa
     2281 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     2341 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     2401 gtcccaccat cgggcgcgga tccctcgagc atgtggtcac atccgcagtt tgaaaaaggt
     2461 agcgcaggta gtgcagcagg tagcggtgca ggttggagcc atcctcagtt tgagaaactg
     2521 gaagttctgt ttcagggtcc gatgttcagc gtagagtcgc tggagcgggc ggagctgtgc
     2581 gagagcctcc tcacttggat ccagacattt aatgtggatg caccatgcca gaccgtggaa
     2641 gatttaacga atggggttgt gatggcccag gttcttcaaa agatagatcc tgcatatttt
     2701 gatgaaaatt ggctaaacag aatcaaaact gaagtaggag ataattggag gctaaagata
     2761 agcaatttaa agaaaatttt aaaaggaatc ttggattata atcatgagat tttaggacag
     2821 caaattaatg actttaccct tcctgatgtg aaccttattg gggagcattc tgatgcagca
     2881 gagcttggaa ggatgcttca gctcatctta ggctgtgctg tgaactgtga acagaagcaa
     2941 gagtacatcc aagccattat gatgatggag gaatctgttc aacatgttgt catgacagcc
     3001 attcaagagc tgatgagtaa agaatctcct gtctctgctg gaaatgatgc ctatgttgac
     3061 cttgatcgtc agctgaagaa aactacagag gaactaaatg aagctttgtc agcaaaggaa
     3121 gaaattgctc aaagatgcca tgaactggat atgcaggttg cagcattgca ggaagagaaa
     3181 agtagtttgt tggcagagaa tcaggtatta atggaaagac tcaatcaatc tgattctata
     3241 gaagacccta acagtccagc aggaagaagg catttgcagc tccagactca attagaacag
     3301 ctccaagaag aaacattcag actagaagca gccaaagatg attatcgaat acgttgtgaa
     3361 gagttagaaa aggagatctc tgaacttcgg caacagaatg atgaactgac cactttggca
     3421 gatgaagctc agtctctgaa agatgagatc gacgtgctga gacattcttc tgataaagta
     3481 tctaaactag aaggtcaagt agaatcttat aaaaagaagc tagaagacct tggtgattta
     3541 aggcggcagg ttaaactctt agaagagaag aataccatgt atatgcagaa tactgtcagt
     3601 ctagaggaag agttaagaaa ggccaacgca gcgcgaagtc aacttgaaac ctacaagaga
     3661 caggtagtag aactacaaaa cagattatcc gaagaatcaa agaaagcaga taaactagat
     3721 tttgaatata agcggctaaa agaaaaagtt gacagtcttc aaaaagaaaa ggacaggctg
     3781 agaacagaaa gggattctct gaaggaaacc attgaagagc ttcgttgtgt acaagctcaa
     3841 gaagggcagc tcacaacaca agggttaatg cctcttggaa gtcaggagtc ttcagacagt
     3901 ctagctgcag agattgttac acctgaaatc agggagaaac ttattcgtct tcagcatgag
     3961 aataagatgt taaagcttaa ccaagaaggt tcggacaatg aaaaaatagc cttattgcag
     4021 agccttctag atgatgcaaa tctacgcaag aatgaactgg agacagagaa taggctggtg
     4081 aatcaaagac ttctggaagt acagtcacaa gttgaagaat tacaaaaatc tttacaggat
     4141 caaggctcaa aagcagaaga ttcagtcctt ctaaaaaaga agcttgaaga acatctagag
     4201 aagctgcatg aggccaataa tgaactacag aagaagagag ccattattga agatctcgag
     4261 ccaagattta acaacagctc cttaaaaatt gaagaattac aagaagcttt acgaaagaaa
     4321 gaggaagaaa tgaagcaaat ggaagaacga tacaaaaaat acttagagaa agccaaaagt
     4381 gtcatccgta ctttagatcc taaacagaat caaggagcag caccagaaat acaagctctt
     4441 aaaaatcagc tccaggaacg agaccgactg ttccactcat tagagaaaga atatgagaaa
     4501 acaaagagtc agagagagat ggaagagaaa tatattgtta gtgcctggta caatatggga
     4561 atgaccctgc ataaaaaggc agctgaagat agactggcaa gcacaggctc agggcagtca
     4621 tttctggcga ggcagaggca agcgaccagc agcagaagat catacccagg ccacgtgcag
     4681 ccggccacag caaggtaagg tacctctaga gcctgcagtc tcgacaagct tgtcgagaag
     4741 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4801 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4861 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4921 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4981 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     5041 agctgagttg gctgctgcca ccgctgagca ataactatca taaccattta aataaactgg
     5101 atactattgc acgtttaaat cgacctactc cggaatatta atagatcatg gagataatta
     5161 aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat
     5221 aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc gcggatccct
     5281 cgagcatgaa ccctttctgg agtatgagca ctagtagtgt ccgtaagcga agtgagggcg
     5341 aagaaaagac gctgactggg gacgtgaaaa cgtccccacc ccgcacagcc ccaaagaagc
     5401 agctcccaag cataccaaag aatgccctgc caattaccaa acccacatcc cctgcacctg
     5461 ccgcacagtc tactaacggt acacacgcct cttacggacc attttatctt gagtacagcc
     5521 ttctggctga atttaccctc gtggtaaagc aaaagctgcc tggcgtctac gtgcagccct
     5581 catacaggag cgccttgatg tggttcggag ttatctttat ccgacatggt ctctatcaag
     5641 atggcgtttt caaattcact gtgtatattc ctgataatta tcccgacgga gactgccctc
     5701 ggctcgtttt cgatattcct gtattccatc cccttgtcga ccccacatct ggtgaactgg
     5761 acgtgaaaag ggccttcgct aagtggagac ggaaccataa tcacatctgg caagtcttga
     5821 tgtatgctcg tagagtcttt tacaaaatcg ataccgcctc tccgttgaac ccggaagccg
     5881 ccgtacttta cgaaaaggac attcaactgt tcaagagtaa ggtagtggat agtgtcaaag
     5941 tgtgtactgc ccgccttttc gaccagccca aaattgagga cccgtacgcc atctctttca
     6001 gtccctggaa ccctagtgtc cacgacgaag ctcgcgaaaa gatgctcacc cagaagaagc
     6061 cagaggaaca gcacaataag tctgtccacg tagcagggct gtcttgggtc aagcctggat
     6121 ctgtgcagcc attttccaag gaagaaaaga cggtggctac ttaaggtacc tctagagcct
     6181 gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     6241 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     6301 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     6361 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6421 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat
     6481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgaggcaata
     6541 actatcataa ccatttaaat aaatctaatg atgcctgatg tttaaatcga cctactccgg
     6601 aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt
     6661 ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata
     6721 ccgtcccacc atcgggcgcg gatccctcga gcatggaaag aatgaattgg ctctcccgtc
     6781 tcgcctctag gggcccaggg cacaggattc cccaaggggc gaatctccaa acccctgtta
     6841 tggccgaccc agaaacctgc ctcatggtat tcaagaacca ttggtcccaa gtggtgagaa
     6901 tactggaacg tcaagggccc cgtgccgccc caggtggggc ggacgacctc agtgccgtca
     6961 gaaatcatac atatcaaatg ctgacccttc tggctgaaga tagggccgtg ccatccgccc
     7021 ccacaggtcc aggcccgctg ttggaattcg ctctccacga ggatctcctg actcgggtcc
     7081 tgacttggca attgcagtgg gacgagcttg gggacggcgt tgaagagcgg agagccgagc
     7141 agctgaagtt gttcgagatg cttgtcagtg aggcacgaca accccttctc agacatggcc
     7201 cggtccgcga agcgcttctg accctgcttg acgcttgcgg ccgccccgtt ccttcaagtc
     7261 ctgcacttga tgaggggctc gtgctcttgc tctcccaact ttgcgtgtgt gtggcacaag
     7321 aacccagtct gttggagttc tttcttcagc caccacccga accaggcgcg gctccccgat
     7381 tgcttctgtt cagccggttg gtgccgtttg ttcatctaga gggcacattg ggccaacaag
     7441 cccgagatgc gctgctcttg ctgatggcgc ttagtgctgg ctcaccaact gttggaaggt
     7501 atattgcaga tcacagctac ttttgccccg tactggctac tgggttgagc gcgctctata
     7561 gctcattgcc gcgtaaaatt gaggtccccg gtgatgattg gcattgtctg cgtagagaag
     7621 attggttggg tgtacccgcc ctggccttgt tcatgagctc actggaattc tgcaacgcgg
     7681 ttatacaagt ggcgcaccca ttggttcaga agcaactggt tgattacatc cataatggtt
     7741 ttctcgtacc tgtcatgggc ccagccttgc ataagaccag tgtggaagaa atgatcgcaa
     7801 gtacagctta tttggaattg ttcttgcgct ccatctcaga gccagccctg ctcagaacct
     7861 ttctccgctt tctcctgctg caccgacatg acactcatac aatcctcgat acgctggtgg
     7921 ctagaattgg atccaatagc cgcttgtgca tggtgagctt gtctctcttt agaacactgc
     7981 ttaatttgtc ctgcgaggat gtcctgttgc aactggtgct gaggtatttg gttccatgta
     8041 accacgttat gttgtcccag aaacccgctg ttcgtgatgt ggacctttat ggcagagccg
     8101 cagataagtt cctcagtctg attccccgtt gctgccggca tcatgccccc tcacccccgc
     8161 gacccgaaca tgcgtcatgg gcccgaggcc ctgggagccc cagcgttgac tctagcagcg
     8221 tgactaccgt ccctcggccc agcactccaa gccgtctggc ccttttcctt cgacaacaaa
     8281 gcctgggcgg atctgaaagt cccgggcccg ccccatgctc acccggtctc tccgcatctc
     8341 cggcatctag tccgggacgt cgacccacgc cagctgagga gcctggggag ctcgaggata
     8401 attatctcga gtacttgagg gaagctagac ggggcgtcga tcgatgtgtt cgcgcctgcc
     8461 gcacttggtc tgcgccttat gacggtgaac ggccgtcccc agaaccttct cccttcggga
     8521 gcaggaccaa gaaacgctcc ctgctcccag aagaagaccg gaataacgtg ggagagggcg
     8581 aagaagagga acttgggcgc agaggtcgcg caggcggcgc gggtgaggga cccggtcatc
     8641 tgccaccacc acaacttaac ggggtgccag gcagttggcc cgaaggcgct aagaaggtaa
     8701 gactggtgcc aaaagaagga gccggagagc ttcttgaagg aatctccgag ggaatggcag
     8761 gattggaagg cttcggtcaa gaacttagag agctcgaggt tgcactgtct aatggtggaa
     8821 cggggagtga aagccccctg gaacccccgc tgcctttgga agaagaggaa gcctatgaga
     8881 gcttcacatg tccccctgag ccgccagggc ccttcctgtc ctcaccactg cgcaccttga
     8941 atcaacttcc ttctcagcca tttactgggc cctttatggc tgtattgttt gctaagctcg
     9001 agaatatgct ccaaaacagc gtctacgtca attttctcct cacaggtctg gtggcacaac
     9061 tggcctgcca cccacagcct ctgctgcgaa gcttcttgct caatactaac atggtgttcc
     9121 agcctagcgt caagagtctg ttgcaagtgc tgggctcagt gaagaataag atagaaaact
     9181 ttgctgccag ccaggaagat ttcccagccc ttctgagtaa agccaagaaa tatcttattg
     9241 cacgtggcaa actggactgg gctgaaggcc ccgcggccgg gccggctccg aggcgttccg
     9301 acccactcgt gaagtctagg cggccgtcac tcggagagct gctcctgcga cacgctcatt
     9361 ctcccaccag ggctagacaa gctgcgcagc tcgtgctgca accgggaagg gatggtgcag
     9421 gcctcggcct cagcggcggg tcccccggag cctcaacccc tgtccttctc acccgcggcg
     9481 gagcgcccga acggcaaggg gaagccctca gagtaaagaa tgctgtctac tgcgcagtga
     9541 tatttccaga gttcttgaaa gagctcgccg ctatttcaca agcacacgca gtgacttccc
     9601 catttctcct tgagacctct gaagaagggt ccggcccgct tatatccggt tgtggaccac
     9661 tcaaccctta aggtacctct agagcctgca gtctcgacaa gcttgtcgag aagtactaga
     9721 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9781 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     9841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9961 tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag
    10021 ttggctgctg ccaccgctga gcaataacta tcataaccat ttaaataaat ctatatctca
    10081 atcggggtta gtcgataccc caccgagacc ccattgggac caatacgccc gcgtttcttc
    10141 cttttcccca ccccaacccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    10201 ggcggcaagc cctgccatag ccactacggg ttaattaaaa actatatctc aatcggggtt
    10261 taaacaggag atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta
    10321 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
    10381 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
    10441 a
//
