LOCUS       sequence-443741-       11796 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express LRRC10 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, 
            Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, 
            David J. Papke, Mateo Valenzuela, Brianna Silverman, Amanda 
            Falzetta, Scott B. Ficarro, Jarrod A. Marto, Christopher D.M. 
            Fletcher, Antonio Perez-Atayde, Thierry Alcindor, Akiko Shimamura, 
            John R. Prensner, Richard I. Gregory, Alejandro Gutierrez
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation
  JOURNAL   Molecular Cell, 2025, ISSN 1097-2765
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, 2025, ISSN 1097-2765"
COMMENT     Sequence Label: rag2:LRRC10_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    5969..6103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6124..6258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6560..7273,7274..7276,7277..7279))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6563..7273,7274..7276,7277..7279))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6605..6626)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6954..6973
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7213..7234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8460..8476)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8460..8476)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8531..8549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8559..8576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8559..8575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8568..8590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8716..9171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8853..8874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9065..9084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9198..9302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9303..9371,9372..10163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9521..9540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10334..10922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10823..10842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11076..11093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11210..11231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11246..11251,11252..11269,11270..11276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11284..11300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11289..11311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11308..11324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11308..11324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11343..11363
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11345..11363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatgss
      721 wrwgstmggt kkggkycywa akscccsrgt ataaaccagc aggtcaacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
      901 aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccwrwrwwr aymarmmrrk
      961 swmcckryks tctgtgkkgg tmmwaawkyc ccagkatrwc cttcctaccc tggcatcctg
     1021 gacaaatttg ctcactgtcc tctgtccatc atggcctcct aaccatcccc atatcataat
     1081 tggctttatc actctagctg gggccgaaat cgcatacttc cattttatga ggtacgctaa
     1141 aatcagtatg cgagccaagt aatatgtccg aatacttccg gcgagattct agagtgtgca
     1201 ttcgatgcac gctacgctat tccatgatgc cccgtgagag aattcttgaa tcggagagaa
     1261 gcaacgcaac tcatgcgggt aggttacgtg acgatgacat attggcggat gtagtacgtc
     1321 caagttccat tcatactgct cacattcata ttttatagaa tgttcttttc ccacagccaa
     1381 gtagtaagtt taaattcaaa tacagtacct actaagtagt agtagacggt ttcggacgca
     1441 gcccctgtct cctctccacc aatatactgg tgtgtagtgt gcggtctggt gcaatatggc
     1501 tgccatcgcg tcatccaggt ggatgctgca cactggtggt tgatgaggag attttgccca
     1561 aaaaagtgtt ttgagtttcc agaaaagcgc gatataaatg taaggaatta tcattagctt
     1621 attataatgg aactttctgg ataacttgtg ccagagctag gggccattaa aactttgccc
     1681 tggggtacct cagggagctg caaggaagtg ctatagggct aatgaaatgt ttgggggaaa
     1741 aaaaaaaatt ttataagtaa aaataaaact aaaataatta ataatttcat tgttgaaaat
     1801 tatagtaaaa ttgataaaca agataagcag cattagtata aaaaactaaa aacaaacgta
     1861 atttaaaata attaaattgt tttaaataaa ttatacaaaa taatgtttac aacgacacgg
     1921 tggtgcagta ggtagtgctg tcgccagcaa agcaagaagg tcgctggttc gagcctcagc
     1981 tggatcactt gatgtttctg tgtggagcat tcgcgtgcgt ttaaccccag attaataaag
     2041 agactaagcc gaaaagaaaa taaatgtatg aaaatgttta cgtaaaatgt aaataaatgc
     2101 atgattaata aaatatatga attttaaaga ttgaggtaga attgttttat tattcacaca
     2161 tttggttatt tttaatcagt gacaacttgt gatgctcttt atattgttta ttacgctact
     2221 ttgaatattc agtctcaaat tgcatgtaaa tacgacttca aagcagaatt agtttaattt
     2281 aattgcctgt aaacagtgtt gtactttgat ggtgattgtc tgataataat atgatttcac
     2341 tatttaaaat ttgaaaaaaa aaaatctgaa aaatactcac cggccacttt attaggtaca
     2401 cctgtctaac tgcttgttaa cgtaaatttc taatcagcca atcaccaggc agcaactcag
     2461 tgcatttagg catgtagaca tggtcaagac gatctgctgc agttcaaagc gagcatcaga
     2521 atggagaagc aaggtgattt aagtgacttt aaacgtgacg tgtttgttgg tgccagacgg
     2581 gctggtctga gtatttcaga aactgctgat ttactggggt tttcacacac aaacatctct
     2641 ggggtttaca gagaatggtc caaaaaaaga taatatccag tgagcggcag ttctgtgggg
     2701 gcaaatgtct tgttgatgcc agaggtcaga ggagaatggc ctgactggtt ccagctgata
     2761 gaaaggcaac agtaattcaa ataaccactc gttagaactg aggcatgcag aagagcacct
     2821 ttgaacacaa catgtcgaac cttgaggcgg gtgggctaca gcagctcaag accacatcgg
     2881 gtgccactcc tgtcagctaa gaacaggaaa ctgaggctac aattcgcaca ggctcaacat
     2941 aagtagacaa tagaagattg aacattatgt tgcctggtca gatggataca ttctaccttg
     3001 tatcaacagt tcaggctggt gatggtggtg taatggtgtg ggggatatat tcttggcaca
     3061 ctttgggccc attagtacca attaaccatc gtgtcaacgc cacagcctaa ctgagtattg
     3121 ttgctgacca tgttcatttc tttttgacca cagtgtaccc atcttctgat ggctacttcc
     3181 tgcaggataa tgcaccatgt cataaagcgt gaatgatctc agactggttt cttgaacatg
     3241 acaatgagtt cactgtgctc gaatgacctc cacagtcacc agatctcaat ccaatagagc
     3301 gcctttgggg tgtggtagaa tggaagattc gcatcctgga tgtgcagccg acaaatctgc
     3361 atgatgctag catgtcaata tggaccaaaa tctctgagga attttccagt acctggttga
     3421 aatgcaaaag gaagggaatc tatgctaagg caaaaagagg tccaacccag tactagtggg
     3481 gtgtacataa taaagtggcc ggtgagtgta tattgttaag gatatctaga taatccaaat
     3541 aatttacagc actatttaaa aaaaaaactt tatgtcacca agtctgcatt tatttgatca
     3601 caattacagt cgaaaaagca atatcgggat acattgtttc catttaaaat aactatatta
     3661 tattttaaat atttttgaca atgttatttc tttaatgcca aagctaaatt gtattcaatc
     3721 tgaattttag tgtcacatga tccttcagaa aatattaaca cattgctaaa aatgtatatt
     3781 attatcaatt tggatttttt tttttttttt gtatattaac ctatgctatt ggttactata
     3841 caaaagtatt actgtgaata tatgcatata cagaaccgta tcaagcgcgg ggggtggttg
     3901 ggttatgtcc ccatttggaa agacctggaa acattcataa tgactaaaat tgcacggaaa
     3961 aatataatat ctatgagtat tgctgatcag cagcacaaaa ccagaactcc atattaataa
     4021 acagccaatg atgcagtgag tttgtgcatg ttttcattca aactcgtgtg attgtgctca
     4081 aacacctgtt gtgagcagca gttgcaaaca gtaaacctca agaggagcca gtcaaccttc
     4141 acaaacactc aacctctggc tcactcacta aacaattcgc gttaatatct aaaataaaaa
     4201 tttccagcac caaacagctc tcagatttcg gagtacactg accaagaggt aagaagtaac
     4261 gttacattac taggacgact attgtaagac tagatatgta atatttggaa gcgtacgtgt
     4321 tttaagtgac atatagagta gaggaactga tggaaatacg atttgcataa tgtaaaatga
     4381 tatatattaa aatataggct acagtaggta acgtggttta catataacgt tactgtgtta
     4441 atgtagtgct cagttactcg tgttgacagt tattattagt gcttgctaat ttgtttgtta
     4501 atgcttctct tgcgtatttg attaccttca ggcgaatcta aaacatatca gatgtagaaa
     4561 cggcagaaaa atgttgaaga aaatacgaga atagtagctt ttggagcaca tagaaaagta
     4621 catcagataa agcctaccgt ttaaacgcag acgccgctct cgcactgaat gctgggtact
     4681 gtagtcctta tattgtatgc ttaatgtgcg tctcaacggg aaactagagt taggaaaact
     4741 acaatttcct gtggaaaatg tttctgtttt cggggaaaca gcgaaagaaa gatatggttc
     4801 cgcgtttttt tcttactttg aataataaaa taagacctaa ctacacactt cgcttactct
     4861 tatgctaatt tgaagcacta gcattctttg atacaatgat aatcatttaa acatcacagt
     4921 tatactacaa atactatggt taaactatgt ctagtgtagt gtaacactat ggttgccatt
     4981 gttatcttta agtttaagta ttatctgtgg taaagccatg gttggttttt ataagaggta
     5041 ataaaattaa cattgtacaa ttaaatgact ataaattaat gttttttttt atttttttta
     5101 cacagctacc ttcaaaaagg gatccatggg gaacaccatc agggccctcg tggccttcat
     5161 ccctgctgac cgttgccaga actatgtggt cagggacctc cgtgagatgc cgctggacaa
     5221 gatggtggat ctgagtggga gccagttacg ccgcttcccc ctgcacgtgt gctccttcag
     5281 ggagctggtc aagctctacc tgagcgacaa ccacctcaat agcctgcctc cggagctggg
     5341 gcagctacag aacctgcaga ttctggcctt ggatttcaac aacttcaagg ctctgcccca
     5401 ggtggtgtgc accttgaaac agctctgcat cctctacctg ggcaacaaca aactctgcga
     5461 cctccccagt gagctgagcc tgctccagaa cctcaggacc ctgtggatcg aggccaactg
     5521 cctcacccag ctgccggatg tggtctgtga gctgagtctc cttaagactc tgcatgccgg
     5581 ctccaacgcc ctgcgtttgc tgccaggcca gctccggcgc ctccaggagc tgaggaccat
     5641 ctggctctcg ggcaaccggc taactgactt tcccactgtg ctgcttcaca tgcccttcct
     5701 ggaggtgatt gatgtggact ggaacagcat ccgttacttc cccagcctgg cgcacctgtc
     5761 aagtctgaag ctggtcatct atgaccacaa tccttgcagg aacgcaccca aggtggccaa
     5821 aggtgtgcgc cgtgtgggga gatgggcaga ggagacgcca gagcccgacc ctagaaaagc
     5881 caggcgctat gcgttggtca gagaggaaag ccaggagcta caggcaccag tccctctact
     5941 tcctcctacc aactcctgaa tcgatgatga tccagacatg ataagataca ttgatgagtt
     6001 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattcgat
     6121 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6181 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     6241 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     6301 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     6361 gatcctctag atcagatctc ttgtttattg cagcttataa tggttacaaa taaagcaata
     6421 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6481 aactcatcaa tgtatcttat catgtctgga tctacgtaat acgactcact atagttctag
     6541 aggctcgaga ggggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     6601 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     6661 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     6721 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     6781 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     6841 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     6901 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     6961 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     7021 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     7081 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     7141 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     7201 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     7261 cttcgccctt gctcaccatg gtcactgtct gctttgctgt tggtctgggc tcctgggtca
     7321 ctggcttact aatggagtct ttatgtatga ggactcttat caattgttct tctataaagg
     7381 tctgcagtgt ttctgttcgt cccctacatg gacacccaga gcctcctaaa tacaggagcc
     7441 ctgataactg cacaagtgct cagattccag cagggtggaa atgagataaa gtgtgcagat
     7501 ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa
     7561 atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta
     7621 cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac
     7681 aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt
     7741 gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga
     7801 catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa
     7861 aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct
     7921 tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt
     7981 gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc
     8041 taaagttaca actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat
     8101 gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca
     8161 caactgattt aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
     8221 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
     8281 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
     8341 tagatgtgac atgattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     8401 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag
     8461 atccactagt tctagagcgg ccattaccct gttatcccta ggccgccacc gcggtggagc
     8521 tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8641 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8701 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8761 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8821 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     8881 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     8941 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     9001 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9061 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9121 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     9181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9541 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11101 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11161 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11221 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    11281 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    11341 gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccccc ctcgaattac
    11401 cctgttatcc ctatcgagca aaatcaatcc taatgtaaaa tagtatacgt tcatcattat
    11461 aatgcaaaca ctgtagaact gttgtgttgt gccttgcatt tcattctcag tttcttatcg
    11521 tctgtctctt tttacgacag agctagaaaa aactagcctt catttgccat cgccgtatgc
    11581 aaatccactc cagaactcaa caggtttgtt ctgcggcggg tgtaagtgtg aacaccggca
    11641 tgtaagtgcg cttgagcagg agaataaagc gctcagagac accggaggaa tcctttattt
    11701 tgcgttgcgc ctcaggggat tcgctgagcc ttctcttcat ggccctgctc ttcagacaca
    11761 ggtgctccgc tgtttgtgtt tttcactgtt cttcag
//
