LOCUS       sequence-443756-       10496 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of full-length TNRC6A with S1616D/S1691D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Munakata F, Suzawa M, Ui-Tei K
  TITLE     Identification of Phosphorylated Amino Acids in Human TNRC6A 
            C-Terminal Region and Their Effects on the Interaction with the 
            CCR4-NOT Complex.
  JOURNAL   Genes (Basel). 2021 Feb 13;12(2):271. doi: 10.3390/genes12020271.
  PUBMED    33668648
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2021-02-13"; volume: "12(2):271. doi"; pages: " 
            10.3390/genes12020271"
COMMENT     Sequence Label: pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      989..998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             998..1021
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1022..1048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1052..1165
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    5395..5406
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6330..6346)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6331..6347)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6402..6421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6403..6421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6448..6465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6454..6678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6811..6830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6962..7009
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7017..8039
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7017..8039
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8169..8290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8206..8225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8260..8279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8339..8355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8339..8355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8352..8374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8363..8379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8387..8393,8394..8411,8412..8417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8432..8453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8570..8587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8741..9329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8821..8840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9500..10291,10292..10360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10123..10142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10361..10465)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
     1021 gtacccatac gatgttccag attacgcttc tatggacgag aagaccaccg gctggcgggg
     1081 cggccacgtg gtggagggcc tggccggcga gctggagcag ctgcgggcca ggctggagca
     1141 ccaccctcag ggccagcggg agccctgccc gggcggatcc atggatgctg attctgcctc
     1201 cagttctgaa tcagagagaa acatcactat catggcttca gggaacacag gtggtgaaaa
     1261 agatggcctt cggaatagca ctggacttgg ttcccaaaac aagtttgtag ttggtagcag
     1321 cagcaataat gtgggccatg gaagtagtac tgggccatgg ggtttttccc atggagccat
     1381 aataagcaca tgtcaggtct ctgtggatgc tcctgaaagc aaatctgaaa gtagcaacaa
     1441 tagaatgaat gcttggggca ctgtaagttc ttcatcaaat ggagggttaa atccaagcac
     1501 tttgaattca gctagcaacc atggtgcctg gccagtatta gagaacaatg gacttgccct
     1561 aaaagggcct gtagggagtg gtagttctgg cattaatatt cagtgcagta ctataggcca
     1621 gatgcctaac aatcagagta ttaactctaa agtgagtggt ggttctaccc atggtacctg
     1681 gggaagcctt caggaaactt gtgaatctga agtaagtggt acacagaagg tttcattcag
     1741 tggtcaacct caaaatatta ccactgaaat gactggacca aataacacta ctaactttat
     1801 gacctctagt ttaccaaact ccggttcagt gcagaataat gagctgccta gtagtaacac
     1861 aggggcctgg cgtgtgagca caatgaatca tcctcagatg caggctccat caggtatgaa
     1921 tggcacttcc ctttctcacc ttagcaatgg agagtcaaaa agtggaggct cttatggtac
     1981 tacatggggt gcctatggtt ctaattactc tggagacaaa tgttcaggcc ctaatggcca
     2041 agctaatggt gacactgtga atgcaactct aatgcagcct ggcgtaaatg gtcctatggg
     2101 cactaacttt caagttaaca caaacaaagg aggtggtgtg tgggaatctg gtgcagcaaa
     2161 ctcccagagt acatcatggg gaagtggaaa tggcgcaaat tctggaggaa gtcgaagagg
     2221 atggggaacc cctgcacaaa acactggcac taatttaccc agcgttgagt ggaacaaact
     2281 gcctagcaat cagcattcca atgatagtgc aaatggcaat ggtaagacgt ttacaaatgg
     2341 atggaaatct actgaggaag aggatcaggg ttctgccaca tctcagacaa atgagcaaag
     2401 cagtgtgtgg gccaaaacag gaggtacagt ggagagcgat ggtagtacag aaagcactgg
     2461 acgccttgag gaaaaaggaa ctggggaaag tcagagtaga gacagaagaa aaattgatca
     2521 gcacacatta ctccaaagca ttgtaaacag aactgactta gatccacgtg tcctgtccaa
     2581 ctctggttgg ggacagactc ctattaagca gaatactgcc tgggatacag aaacatcacc
     2641 tagaggggaa cgaaagactg acaatgggac agaggcctgg ggaagctctg caacacagac
     2701 ttttaactca ggggcatgta tagataagac tagccctaat ggtaatgata cctcatctgt
     2761 atcagggtgg ggcgatccca aacctgctct gaggtgggga gattccaaag gctcaaactg
     2821 ccaggggggg tgggaagatg attctgctgc tacaggaatg gtcaagagca atcagtgggg
     2881 gaattgcaaa gaggagaagg ctgcatggaa tgactcgcaa aagaataaac agggatgggg
     2941 tgatggacaa aaatcaagcc aagggtggtc tgtttctgcc agtgataact ggggagaaac
     3001 ttcaaggaat aaccattggg gtgaggccaa taagaaatcc agctcaggag gtagtgacag
     3061 tgacaggtcc gtttccggtt ggaacgaact tggtaaaact agttctttca cttggggaaa
     3121 caacataaat ccaaataatt catcaggatg ggatgaatct tctaaaccta ctccttccca
     3181 gggatgggga gaccctccaa agtctaatca gtctctaggt tggggagatt cgtcaaagcc
     3241 agtcagctct ccagactgga acaagcaaca agacattgtt ggatcttggg gaatcccacc
     3301 agctacaggc aaacctcctg gtacaggctg gctgggggga cctataccag ccccagcaaa
     3361 agaagaagaa cccacaggct gggaggaacc atccccagaa tctatacgtc gcaaaatgga
     3421 gattgatgat ggaacttcag cttggggaga tccaagcaaa tacaactaca aaaatgtgaa
     3481 catgtggaac aaaaacgtcc caaatggcaa cagccgttca gaccagcaag cacaggtaca
     3541 tcagctgcta acgcctgcaa gtgccatctc aaacaaagag gcaagcagtg gctctggctg
     3601 gggtgagccc tggggggagc cttctactcc agccacaact gtggataatg gtacttcagc
     3661 atggggtaag cccatagaca gtggtcccag ctggggggaa cccattgctg cggcatccag
     3721 cacatccacg tggggctcca gctctgttgg tccacaagca ttaagcaaat ctgggccaaa
     3781 atctatgcaa gatggctggt gtggtgatga tatgccattg cctggaaatc gccccactgg
     3841 ctgggaagag gaagaggatg tggagattgg aatgtggaat agtaattcat ctcaagagct
     3901 taactcatct ttaaattggc caccatatac aaagaaaatg tcatcgaagg gtctgagtgg
     3961 caaaaaaagg agaagggaaa ggggaatgat gaaaggtgga aacaaacaag aagaagcgtg
     4021 gataaatcca tttgttaaac agttttcaaa catcagtttt tcgagagact caccagagga
     4081 aaatgtacaa agcaataaga tggacctttc tggaggaatg ttacaagaca aacgaatgga
     4141 gatagataaa catagcctaa atattggtga ttacaatcga acggtcggga aaggccctgg
     4201 ttctcggcct cagatttcca aagagtcttc catggagcgc aatccttatt ttgataagga
     4261 tggcattgta gcagatgaat cccaaaacat gcagtttatg tccagtcaaa gcatgaagct
     4321 tcccccttca aatagtgcac tacctaacca ggcccttggc tccatagcag ggctgggtat
     4381 gcaaaacttg aattctgtta gacagaatgg caatcccagt atgtttggtg ttggaaacac
     4441 agcagcacaa ccccggggca tgcagcagcc tccagcacaa cctcttagtt catctcagcc
     4501 taatctccgt gctcaagtgc ctcctccatt actctcccct caggttccag tttcattgct
     4561 gaagtatgca ccaaacaacg gtggcctgaa tccactcttt ggccctcaac aggtagccat
     4621 gctgaaccag ctatcccagc taaaccagct ttctcagatc tcccagttac agcgattgtt
     4681 agcgcagcag caaagggcgc agagtcagag aagcgtgcct tctgggaacc ggccgcagca
     4741 agaccagcag ggtcgacctc ttagtgtgca gcagcaaatg atgcaacaat ctcgtcaact
     4801 tgatccaaac ctgttggtga agcagcagac tccaccatct cagcagcagc cactccatca
     4861 gccagccatg aagtctttcc ttgacaatgt catgccccac actacacctg agctgcaaaa
     4921 agggccatca ccaataaatg ctttcagcaa cttccctata ggcttgaact caaacttgaa
     4981 tgtaaatatg gatatgaaca gtattaaaga gccacagtca agactaagga agtggacgac
     5041 agtggacagc atttctgtga acacatcttt ggatcaaaac tccagcaaac atggtgctat
     5101 ttcaagtggt ttcaggctgg aagagtctcc atttgttccc tatgacttta tgaacagcag
     5161 tacttcacca gccagtcctc caggttcaat aggagatggc tggccacgtg ccaaatcgcc
     5221 taacggctct agcagtgtta attggccacc agaatttcgt cctggtgagc catggaaagg
     5281 ttatccaaac attgaccctg aaactgaccc ttacgtcact cctggcagtg tcataaacaa
     5341 tctttcaatt aatactgtgc gggaagttga ccacctcagg gacaggaaca gtgggtcatc
     5401 ctcatccttg aacaccacgc tgccttcaac tagtgcctgg tcatccattc gtgcctccaa
     5461 ctacaacgtt cccctcagca gtacagcaca aagcacttca gccagaaata gtgattccaa
     5521 attgacatgg tctcctggtt cagttacaaa cacctctctg gctcatgagc tgtggaaggt
     5581 ccctttgcca cctaaaaaca tcactgctcc gtcccgccca cctccgggac tgactggtca
     5641 gaagccaccc ttgtctacgt gggataattc tccccttcgt ataggtggag gatggggaaa
     5701 ttctgacgcc agatataccc caggttccag ctggggtgag agcagctcag ggagaataac
     5761 aaattggctt gttctaaaaa accttacacc tcagatcgat ggctcaactc tgcgcactct
     5821 gtgcatgcag cacggcccgc tgatcacatt ccacctgaac ctccctcacg gaaatgctct
     5881 ggtccgctac agttcaaaag aagaggtagt gaaggcacaa aagtctctgc acatgtgtgt
     5941 actggggaac actactattc ttgctgagtt tgccagtgaa gaggagatca gtcgtttctt
     6001 tgcacaaagc cagtctctga cccctgatcc cggctggcag tctctcgggt ccagccagag
     6061 ccggctgggc tccctcgact gttcccactc attctccagc cggaccgatc tcaatcactg
     6121 gaatggtgct gggctgtcgg gaactaactg tggagacctt cacggcactt cactctgggg
     6181 gaccccgcat tattccacaa gcctgtgggg tcccccaagc agcagcgacc cccgaggaat
     6241 tagcagccca gatcccatta acgcttttct ttctgttgac cacctgggtg ggggtggaga
     6301 gtccatgtaa tctaggatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
     6361 accttaatta attaaggtac caggtaagtg tacccaattc gccctatagt gagtcgtatt
     6421 acaattcact cgaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6481 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6541 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6601 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6661 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6721 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     6961 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     7021 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7081 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7141 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7201 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7261 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7321 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7381 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     7441 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     7501 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     7561 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     7621 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     7681 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     7741 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     7801 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     7861 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     7921 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     7981 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     8041 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8101 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8161 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8281 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8341 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8401 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8461 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8521 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8581 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8641 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8701 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9181 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10081 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
