LOCUS       sequence-44413-e         889 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE6
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..64)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(86..235)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(260..280)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcctgctcac tgggccagga ttctcctcga cgtcaccgca tgttagcaga cttcctctgc
       61 cctctccact gcctctagag ttaatcagca tgtccaggtc gaaatcatca agggcgtcac
      121 tgccgagcat gtcgaggtca aagtcatcaa gggcatccga accaagcatg tcaaggtcaa
      181 aatcatcgag ggcgtcactt cccagcatat ccagatcaaa atcgtccaat gcgtcagccc
      241 gtccggaacc gctggcctcc acctttctct tcttcttggg gctagctgac gcgcgagttt
      301 ggtccccttc atgcatgggc gagggcgcgt ccaaatccct ctccagcgag tctgcgaacg
      361 catgaaggct cgcctggtca ggtgtctgag ttgacgtagg tgaaggcttc gcgcgtttca
      421 taccgctcgc ttggagaatc ctgtcccacc gctgggaggc gggaggcagt gtgcccgagc
      481 gggcctcgag ttctgtgaca ccgactctac gaaagagctg cagcagtccg tgtctcgaca
      541 taccaaattg agtcatggcg tcatcgaacg cttgtgcggg gtgggagtga cactggaaga
      601 atccgagcac gcggaccact tgcgcgtgat ctgccactcg atgtgatgtc ctctcgggaa
      661 tccttctgtt ggtccgctta atcaatgcag gcgcgtgcgg gagccccttc ttcaccgcat
      721 ccaaggcggg tcgtccgccg aggcaggcca gcgctacaag atgatcatta gtgagtgcgg
      781 ccagcgcggg gtccggcctc gaaagctggg ccacgattga ctccagtgcg ggtctgcccc
      841 cgatattgga tgcaatagcc actacctgct caggcgtgag tccgtgcgc
//
