LOCUS       sequence-444183-        7046 bp    DNA     circular SYN 15-APR-2025
DEFINITION  NBU2 backbone with anti-LacI(YQR) and P(O1)_nanoluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH111
FEATURES             Location/Qualifiers
     source          1..7046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(391..1473)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHALSQIVAAIKSRAYQLGASVFVSMVERSGI
                     EACKTAVHNLLAQRVSGLIINYPLDNQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITETGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1435..1454
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    2498..2514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2531..2547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      2621..3009
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            3180..3289
                     /note="incP origin of transfer"
     CDS             complement(join(3420..4211,4212..4280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTCELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4043..4062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcagtaccca tgcaggaaaa aggacattca tctgcaatgc gctggctctc ggaatcccgg
       61 cacaggtggt catgaaatgg acgggacaca gcgactacaa agctatgaaa ccctacattg
      121 acatagcgga tgatattaag gcaaatgcca tgaacaagtt taatcaacta taaaagtatc
      181 aaacagtttc atagatagtt accaatgact atatttgtag aaattatcat cacaatatga
      241 gcaacaagga atccaacgta gagaaaacga attttgatgc attcatacaa gcggtcggtt
      301 cggaaataga acaggcacaa gtaggaccaa aacgaaaaaa ggcccccctt tcgggaggcc
      361 tcttttctgg aatttggtac cgaggaaagc tcactgcccg ctttccagtc gggaaacctg
      421 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      481 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
      541 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
      601 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
      661 cactaccgag atatccgcac caacgcgcag cccggtctcg gtaatggcgc gcattgcgcc
      721 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
      781 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
      841 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
      901 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
      961 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     1021 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     1081 ctggttatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     1141 caccgccgtt ttacaggctt cgatgccgct tcgttctacc atcgacacga acacgctggc
     1201 acccagttga taggcgcgag atttaatcgc cgcgacaatt tgcgacagcg cgtgcagggc
     1261 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     1321 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     1381 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     1441 ctctgcgaca tcgtataacg ttactggttt cactagtgcc atatgttaaa aacagatttg
     1501 gagtgcaaag ttacgacaaa taatttgtta acatacatat ttaggcaact ttttcactat
     1561 attttcaact aataaaacaa aaaagaatgc cgacaagttg tgtgacggca tcctttttct
     1621 atttcattag cttatcctac aatcttctta ccttaattta taatttattc ctgagtcaga
     1681 tcaactgcaa agtatccgcg tttaccggat gggaatacac ctgtcaggaa caatacctga
     1741 ttcggagact taacagcagc tttcattgct tcttccagat caaggctgat tgttgcagcc
     1801 aatgcacaaa tgctgttcca tcactggaag ataggcaatt agtagaagca atttgattag
     1861 taatctaaaa gcactccgat aacgattaga gtgcttttag attgtttatc attaattatc
     1921 aaagcaagtg cagttcatta tgccaggatg cgctcgcaca gacgccaacc cgtgacaccg
     1981 ttgatggtca cgcggaacag caggctgccg tccgggttaa tcagacgctc gtcaataatc
     2041 ttattgccat tccacagggt accggtgacg gtgatctttt taccgtcaaa aacggcaatg
     2101 ccttcatacg gacggccgaa atagtcaatc atattcgggg tcacaccatc aatcaccagc
     2161 gtaccgtaat gcaagataac cttgaagtgg tgatcgtcaa ctgggtagac gactttaaag
     2221 attttctcga tttgacccat ttggtcaccg ctcaggcctt cgtacgggat gatcacatga
     2281 atatcaattt tcagaccgtt ctcgccggac agaacgatgc gctgaatcgg cgtcacgctc
     2341 acacccaggt tctggaacag agagcttacg ccaccctgtt ccaggacttg atccaaatta
     2401 taacccgcgg tctgacgcca atcgccaaca aaatcttcca gagtaaaaac catgtttata
     2461 ttatttatat ttgtttgacg agaatatctt tatttaattg ttatccgctc acaattgccg
     2521 acaaaggtaa ttgttatccg ctcacaattc aaataaatag atagaaaaat aacagtttgt
     2581 cgaattttct ttgtaccacc gcggtggagg ggaattccca tgtcagccgt taagtgttcc
     2641 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
     2701 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt
     2761 cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt
     2821 tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt
     2881 agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga
     2941 gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat
     3001 cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgttttc cgctgcataa
     3061 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     3121 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     3181 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     3241 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     3301 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     3361 cctatcacgg aattgatccc cctcgaattg acgcgttatg agtaaacttg gtctgacagt
     3421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3661 tctattaatt gttgccggga agctagagta agtagttcgc aagttaatag tttgcgcaac
     3721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4261 cggaaatgtt gaatactcat actcttcctt tttcaatcat ggccgcggga ttaaaagtcg
     4321 gggattggtg aacaaaaagg tgtttctctc tttaagagaa atatcgtttt gctaaacagt
     4381 tgatattgag gtatcatttt atcgtaaaag acatttttgc tcaacaattg cttgacggaa
     4441 atcaacaaat tttagcattt tgtaaaaaag tcgctatata atttggtgaa ttggagttat
     4501 tttcatattt ttgcatcccg aagagtttct cttaaagaga gaaacatctt ttgcatacct
     4561 tttccgaccg aatttttatg tcgtaaagag gggctttgca gggggtggac tcagaaagat
     4621 gagaatagat gactattgta gttgaaacac atagaaagtt gctgatatac agaccgatac
     4681 gcatatcggg atgaaccatg agtacgttct tttctcaaaa aacataaata ttcgaaaaga
     4741 gatgcaataa attaaggaga ggttataatg aacaaagtaa atataaaaga tagtcaaaat
     4801 tttattactt caaaatatca catagaaaaa ataatgaatt gcataagttt agatgaaaaa
     4861 gataacatct ttgaaatagg tgcagggaaa ggtcatttta ctgctggatt ggtaaagaga
     4921 tgtaattttg taacggcgat agaaattgat tctaaattat gtgaggtaac tcgtaataag
     4981 ctcttaaatt atcctaacta tcaaatagta aatgatgata tactgaaatt tacatttcct
     5041 agccacaatc catataaaat atttggcagc ataccttaca acataagcac aaatataatt
     5101 cgaaaaattg tttttgaaag ttcagccaca ataagttatt taatagtgga atatggtttt
     5161 gctaaaatgt tattagatac aaacagatca ctagcattgc tgttaatggc agaggtagat
     5221 atttctatat tagcaaaaat tcctaggtat tatttccatc caaaacctaa agtggatagc
     5281 acattaattg tattaaaaag aaagccagca aaaatggcat ttaaagagag aaaaaaatat
     5341 gaaacttttg taatgaaatg ggttaacaaa gagtacgaaa aactgtttac aaaaaatcaa
     5401 tttaataaag ctttaaaaca tgcgagaata tatgatataa acaatattag tttcgaacaa
     5461 tttgtatcgc tatttaatag ttataaaata tttaacggct aaaaacaata ggccacatgc
     5521 aactgtaaat gtttacgcgt cctcggtacc aacaaatact ttcaggacga tgtaaagtcc
     5581 tgagaaatca aactgaatgg atagataaat attagtcttt tctcatcgga ttcttttaat
     5641 atcgctactc ttttattatg tagtggttgt attatcttaa ataaaacaaa aacacccgac
     5701 tttacattct tgggtgtaaa acagggtgtt tgttttgcaa tctgctgatt ttcagcgttt
     5761 tttgcggaga gacaggctct cgaacttata ctttcacaga atatcataaa actgcaaacg
     5821 actgataatc aagtgtaaat tacaatatag agtaaaagta aatgtttcta aatgtctttt
     5881 gctatctcaa taattgggtg tatatttggg tgtagaattt caaacgcacc caattatgaa
     5941 tatcaagcgc aacatcattt ttgcattgga gagccggaaa aagaacggtg tgccaatcgt
     6001 agagaacgta cccatccgta tgcgtgtcat ctttgccagc caacgtatcg agtttacaac
     6061 gggctaccgg attgacgtag ccaaatggga tgtagataag cagcgggtaa agaacggatg
     6121 taccaacaag ctaaagcaaa gtgcagccga aatcaatacg gacttgctga aatactatgc
     6181 cgaaatccag aatattttca aggaatttga ggtgcaggag gtcatgccaa cgacccaaca
     6241 gttgaaggaa gctttcaaca tgagaatgaa agacaccagt gaagaacagc cggaagaagc
     6301 ccctgtcagc ttttgggagg tgttcgatga gtttgtaaaa gagtgcggta accagaataa
     6361 ctggacggca tccacctatg aaaaatttgc agcagtgagg aaccacctca aagagttcaa
     6421 ggaggatgca acgttcaact atttcaacga gtttggattg aacgaatacg tcaacttcct
     6481 gcgtgacacc aaggatatga gaaacagcac catcggcaag caaatgggat tcctcaaatg
     6541 gttcctgcgc tggagcttca agaaaggaca tcatcagaac attgcatacg atacgttcaa
     6601 accgaaactg aaaaccacct cgaaaaaagt aatcttcctg acttgggatg aactgaacaa
     6661 gctgaaagac taccagatac ccaaggataa gcaatacctg gaacgtgtgc gtgatgtttt
     6721 cctgttctgc tgctttacga gtttgcggta ttcggatgtt cgcaatctga aaagaagcga
     6781 tgtgaagtcc gaccacatcg aaataaccac agtcaagact gccgacagcc tgacgattga
     6841 actgaacaaa tacagcaaag ccatactgga caaatacaag gacatccatt tcgagaatta
     6901 catggctctg cccgtcatca gcaaccagaa gatgaacgat tacctgaaag agctgggtga
     6961 actggcagaa atcaacgagc ctgtacggga aacctactac aagggaaatg aacgtattga
     7021 tgaagtcaca cccaaatacg ctttgc
//
