LOCUS       sequence-444190-       12803 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express CPM gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, 
            Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, 
            David J. Papke, Mateo Valenzuela, Brianna Silverman, Amanda 
            Falzetta, Scott B. Ficarro, Jarrod A. Marto, Christopher D.M. 
            Fletcher, Antonio Perez-Atayde, Thierry Alcindor, Akiko Shimamura, 
            John R. Prensner, Richard I. Gregory, Alejandro Gutierrez
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation
  JOURNAL   Molecular Cell, 2025, ISSN 1097-2765
REFERENCE   2  (bases 1 to 12803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, 2025, ISSN 1097-2765"
COMMENT     Sequence Label: rag2:CPM_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             908..1007
                     /estimated_length=100
     polyA_signal    7043..7124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7145..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7581..8294,8295..8297,8298..8300))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7584..8294,8295..8297,8298..8300))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7626..7647)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7975..7994
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8234..8255
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9481..9497)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9481..9497)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9552..9570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9580..9597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9580..9596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9589..9611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9737..10192)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9874..9895)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10086..10105
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10219..10323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10324..10392,10393..11184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10542..10561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11355..11943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11844..11863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12097..12114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12231..12252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12267..12272,12273..12290,12291..12297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12305..12321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12310..12332
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12329..12345
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12329..12345
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12364..12384
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12366..12384
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt gggtcctaat
     1021 gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
     1081 aaccagcagg tctacctgtg gtctgtgtgg gtcctaatgc cccagtataa accaacaggt
     1141 caacctgtga tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
     1201 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
     1261 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca
     1321 gtatattgat ggagactcta tactgctcgg tgagctccat ctttcggata agatgttaaa
     1381 acaaggtccc gactctctgt ggtggtaaaa aattcccagg atgtccttcc taccctggca
     1441 tcctggacaa atttgctcac tgtcctctgt ccatcatggc ctcctaacca tccccatatc
     1501 ataattggct ttatcactct agctggggcc gaaatcgcat acttccattt tatgaggtac
     1561 gctaaaatca gtatgcgagc caagtaatat gtccgaatac ttccggcgag attctagagt
     1621 gtgcattcga tgcacgctac gctattccat gatgccccgt gagagaattc ttgaatcgga
     1681 gagaagcaac gcaactcatg cgggtaggtt acgtgacgat gacatattgg cggatgtagt
     1741 acgtccaagt tccattcata ctgctcacat tcatatttta tagaatgttc ttttcccaca
     1801 gccaagtagt aagtttaaat tcaaatacag tacctactaa gtagtagtag acggtttcgg
     1861 acgcagcccc tgtctcctct ccaccaatat actggtgtgt agtgtgcggt ctggtgcaat
     1921 atggctgcca tcgcgtcatc caggtggatg ctgcacactg gtggttgatg aggagatttt
     1981 gcccaaaaaa gtgttttgag tttccagaaa agcgcgatat aaatgtaagg aattatcatt
     2041 agcttattat aatggaactt tctggataac ttgtgccaga gctaggggcc attaaaactt
     2101 tgccctgggg tacctcaggg agctgcaagg aagtgctata gggctaatga aatgtttggg
     2161 ggaaaaaaaa aaattttata agtaaaaata aaactaaaat aattaataat ttcattgttg
     2221 aaaattatag taaaattgat aaacaagata agcagcatta gtataaaaaa ctaaaaacaa
     2281 acgtaattta aaataattaa attgttttaa ataaattata caaaataatg tttacaacga
     2341 cacggtggtg cagtaggtag tgctgtcgcc agcaaagcaa gaaggtcgct ggttcgagcc
     2401 tcagctggat cacttgatgt ttctgtgtgg agcattcgcg tgcgtttaac cccagattaa
     2461 taaagagact aagccgaaaa gaaaataaat gtatgaaaat gtttacgtaa aatgtaaata
     2521 aatgcatgat taataaaata tatgaatttt aaagattgag gtagaattgt tttattattc
     2581 acacatttgg ttatttttaa tcagtgacaa cttgtgatgc tctttatatt gtttattacg
     2641 ctactttgaa tattcagtct caaattgcat gtaaatacga cttcaaagca gaattagttt
     2701 aatttaattg cctgtaaaca gtgttgtact ttgatggtga ttgtctgata ataatatgat
     2761 ttcactattt aaaatttgaa aaaaaaaaat ctgaaaaata ctcaccggcc actttattag
     2821 gtacacctgt ctaactgctt gttaacgtaa atttctaatc agccaatcac caggcagcaa
     2881 ctcagtgcat ttaggcatgt agacatggtc aagacgatct gctgcagttc aaagcgagca
     2941 tcagaatgga gaagcaaggt gatttaagtg actttaaacg tgacgtgttt gttggtgcca
     3001 gacgggctgg tctgagtatt tcagaaactg ctgatttact ggggttttca cacacaaaca
     3061 tctctggggt ttacagagaa tggtccaaaa aaagataata tccagtgagc ggcagttctg
     3121 tgggggcaaa tgtcttgttg atgccagagg tcagaggaga atggcctgac tggttccagc
     3181 tgatagaaag gcaacagtaa ttcaaataac cactcgttag aactgaggca tgcagaagag
     3241 cacctttgaa cacaacatgt cgaaccttga ggcgggtggg ctacagcagc tcaagaccac
     3301 atcgggtgcc actcctgtca gctaagaaca ggaaactgag gctacaattc gcacaggctc
     3361 aacataagta gacaatagaa gattgaacat tatgttgcct ggtcagatgg atacattcta
     3421 ccttgtatca acagttcagg ctggtgatgg tggtgtaatg gtgtggggga tatattcttg
     3481 gcacactttg ggcccattag taccaattaa ccatcgtgtc aacgccacag cctaactgag
     3541 tattgttgct gaccatgttc atttcttttt gaccacagtg tacccatctt ctgatggcta
     3601 cttcctgcag gataatgcac catgtcataa agcgtgaatg atctcagact ggtttcttga
     3661 acatgacaat gagttcactg tgctcgaatg acctccacag tcaccagatc tcaatccaat
     3721 agagcgcctt tggggtgtgg tagaatggaa gattcgcatc ctggatgtgc agccgacaaa
     3781 tctgcatgat gctagcatgt caatatggac caaaatctct gaggaatttt ccagtacctg
     3841 gttgaaatgc aaaaggaagg gaatctatgc taaggcaaaa agaggtccaa cccagtacta
     3901 gtggggtgta cataataaag tggccggtga gtgtatattg ttaaggatat ctagataatc
     3961 caaataattt acagcactat ttaaaaaaaa aactttatgt caccaagtct gcatttattt
     4021 gatcacaatt acagtcgaaa aagcaatatc gggatacatt gtttccattt aaaataacta
     4081 tattatattt taaatatttt tgacaatgtt atttctttaa tgccaaagct aaattgtatt
     4141 caatctgaat tttagtgtca catgatcctt cagaaaatat taacacattg ctaaaaatgt
     4201 atattattat caatttggat tttttttttt tttttgtata ttaacctatg ctattggtta
     4261 ctatacaaaa gtattactgt gaatatatgc atatacagaa ccgtatcaag cgcggggggt
     4321 ggttgggtta tgtccccatt tggaaagacc tggaaacatt cataatgact aaaattgcac
     4381 ggaaaaatat aatatctatg agtattgctg atcagcagca caaaaccaga actccatatt
     4441 aataaacagc caatgatgca gtgagtttgt gcatgttttc attcaaactc gtgtgattgt
     4501 gctcaaacac ctgttgtgag cagcagttgc aaacagtaaa cctcaagagg agccagtcaa
     4561 ccttcacaaa cactcaacct ctggctcact cactaaacaa ttcgcgttaa tatctaaaat
     4621 aaaaatttcc agcaccaaac agctctcaga tttcggagta cactgaccaa gaggtaagaa
     4681 gtaacgttac attactagga cgactattgt aagactagat atgtaatatt tggaagcgta
     4741 cgtgttttaa gtgacatata gagtagagga actgatggaa atacgatttg cataatgtaa
     4801 aatgatatat attaaaatat aggctacagt aggtaacgtg gtttacatat aacgttactg
     4861 tgttaatgta gtgctcagtt actcgtgttg acagttatta ttagtgcttg ctaatttgtt
     4921 tgttaatgct tctcttgcgt atttgattac cttcaggcga atctaaaaca tatcagatgt
     4981 agaaacggca gaaaaatgtt gaagaaaata cgagaatagt agcttttgga gcacatagaa
     5041 aagtacatca gataaagcct accgtttaaa cgcagacgcc gctctcgcac tgaatgctgg
     5101 gtactgtagt ccttatattg tatgcttaat gtgcgtctca acgggaaact agagttagga
     5161 aaactacaat ttcctgtgga aaatgtttct gttttcgggg aaacagcgaa agaaagatat
     5221 ggttccgcgt ttttttctta ctttgaataa taaaataaga cctaactaca cacttcgctt
     5281 actcttatgc taatttgaag cactagcatt ctttgataca atgataatca tttaaacatc
     5341 acagttatac tacaaatact atggttaaac tatgtctagt gtagtgtaac actatggttg
     5401 ccattgttat ctttaagttt aagtattatc tgtggtaaag ccatggttgg tttttataag
     5461 aggtaataaa attaacattg tacaattaaa tgactataaa ttaatgtttt tttttatttt
     5521 ttttacacag ctaccttcaa aaagggatcc atggacttcc cgtgcctctg gctagggctg
     5581 ttgctgcctt tggtagctgc gctggatttc aactaccacc gccaggaagg gatggaagcg
     5641 tttttgaaga ctgttgccca aaactacagt tctgtcactc acttacacag tattgggaaa
     5701 tctgtgaaag gtagaaacct gtgggttctt gttgtggggc ggtttccaaa ggaacacaga
     5761 attgggattc cagagttcaa atacgtggca aatatgcatg gagatgagac tgttgggcgg
     5821 gagctgctgc tccatctgat tgactatctc gtaaccagtg atggcaaaga ccctgaaatc
     5881 acaaatctga tcaatagtac ccggatacac atcatgcctt ccatgaaccc agatggattt
     5941 gaagccgtca aaaagcctga ctgttattac agcatcggaa gggaaaatta taaccagtat
     6001 gacttgaatc gaaatttccc cgatgctttt gaatataata atgtctcaag gcagcctgaa
     6061 actgtggcag tcatgaagtg gctgaaaaca gagacgtttg tcctctctgc aaacctccat
     6121 ggtggtgccc tcgtggccag ttacccattt gataatggtg ttcaagcaac tggggcatta
     6181 tactcccgaa gcttaacgcc tgatgatgat gtttttcaat atcttgcaca tacctatgct
     6241 tcaagaaatc ccaacatgaa gaaaggagac gagtgtaaaa acaaaatgaa ctttcctaat
     6301 ggtgttacaa atggatactc ttggtatcca ctccaaggtg gaatgcaaga ttacaactac
     6361 atctgggccc agtgttttga aattacgttg gagctgtcat gctgtaaata tcctcgtgag
     6421 gagaagcttc catccttttg gaataataac aaagcctcat taattgaata tataaagcag
     6481 gtgcacctag gtgtaaaggg tcaagttttt gatcagaatg gaaatccatt acccaatgta
     6541 attgtggaag tccaagacag aaaacatatc tgcccctata gaaccaacaa atatggagag
     6601 tattatctcc ttctcttgcc tgggtcttat ataataaatg ttacagtccc tggacatgat
     6661 ccacacatca caaaggtgat tattccggag aaatcccaga acttcagtgc tcttaaaaag
     6721 gatattctac ttccattcca agggcaattg gattctatcc cagtatcaaa tccttcatgc
     6781 ccaatgattc ctctatacag aaatttgcca gaccactcag ctgcaacaaa gcctagtttg
     6841 ttcttatttt tagtgagtct tttgcacata ttcttcaaat aaatcgatga tgatccagac
     6901 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6961 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag attgatgagt
     7021 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     7081 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattcga
     7141 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7201 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7261 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     7321 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     7381 tgatcctcta gatcagatct cttgtttatt gcagcttata atggttacaa ataaagcaat
     7441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7501 aaactcatca atgtatctta tcatgtctgg atctacgtaa tacgactcac tatagttcta
     7561 gaggctcgag aggggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     7621 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     7681 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     7741 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     7801 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     7861 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     7921 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     7981 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     8041 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     8101 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     8161 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     8221 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     8281 tcttcgccct tgctcaccat ggtcactgtc tgctttgctg ttggtctggg ctcctgggtc
     8341 actggcttac taatggagtc tttatgtatg aggactctta tcaattgttc ttctataaag
     8401 gtctgcagtg tttctgttcg tcccctacat ggacacccag agcctcctaa atacaggagc
     8461 cctgataact gcacaagtgc tcagattcca gcagggtgga aatgagataa agtgtgcaga
     8521 tggggagggg gacgtgaatg agagatttga gggatgaaaa ggatggatga acgcattgaa
     8581 aatagcccct ttcacaaaat aataccagta aattgtcata taattaatga ccagaccttt
     8641 acctgtaaat gatgtgatgt tcatatacag aagaatgcca acaatttaca gatgatttta
     8701 caaattctta attttccttt tctaaatgga tttgttcaca cattatctct tcaaagcaat
     8761 tgaattttct ggaaaagatt ggctgtgtgt gtaaaaggtg ctactgagga atttaatgtg
     8821 acatggaatg aagcaaacag cagcctaaga caggatggca ggaaaaatgt cacatgttta
     8881 aaataagagt gaaaccaacg acctggactt atagagactg tgtgtcctta ggcaaagctc
     8941 ttgtacaaac agtgcttgtg actgtgaaat tcggggtttg cctggattgt gttaaatgtg
     9001 tgcttggatg tatcataaaa aagaatcggt tctctgtatt tgacattttc tgtaatttat
     9061 ctaaagttac aactggctat gcctgatttt tatcactatt gaatcaatca ttttaaagaa
     9121 tgttaagtct gctgatttgg agagctcatt tacatttatt ttgaatgtct cttatttaac
     9181 acaactgatt taagctttag atctgcgaag atacggccac gggtgctctt gatcctgtgg
     9241 ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt
     9301 ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta
     9361 atagatgtga catgattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     9421 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca
     9481 gatccactag ttctagagcg gccattaccc tgttatccct aggccgccac cgcggtggag
     9541 ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     9601 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     9661 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9721 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     9781 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     9841 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     9901 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     9961 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
    10021 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
    10081 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
    10141 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
    10201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10321 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10381 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10441 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10501 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10561 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10621 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10681 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10741 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10801 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10861 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10921 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10981 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11041 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11101 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11161 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11221 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11281 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11341 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11401 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11461 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    11521 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11581 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11641 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11701 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11761 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11821 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11881 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11941 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12001 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12061 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    12181 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    12241 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    12301 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    12361 cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaatta
    12421 ccctgttatc cctatcgagc aaaatcaatc ctaatgtaaa atagtatacg ttcatcatta
    12481 taatgcaaac actgtagaac tgttgtgttg tgccttgcat ttcattctca gtttcttatc
    12541 gtctgtctct ttttacgaca gagctagaaa aaactagcct tcatttgcca tcgccgtatg
    12601 caaatccact ccagaactca acaggtttgt tctgcggcgg gtgtaagtgt gaacaccggc
    12661 atgtaagtgc gcttgagcag gagaataaag cgctcagaga caccggagga atcctttatt
    12721 ttgcgttgcg cctcagggga ttcgctgagc cttctcttca tggccctgct cttcagacac
    12781 aggtgctccg ctgtttgtgt ttt
//
