LOCUS       sequence-444515-       11991 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway vector for 35s promoter-driven expression in Marchantia 
            polymorpha carrying a sulfadiazine selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Robinson K, Chia KS, Guyon A, Schornack S, Carella P
  TITLE     An Efficient Sulfadiazine Selection Scheme for Stable Transformation
            in the Model Liverwort Marchantia polymorpha.
  JOURNAL   J Exp Bot. 2024 Jun 2:erae256. doi: 10.1093/jxb/erae256.
  PUBMED    38824404
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2024 Jun 2:erae256. doi: 10.1093/jxb/erae256."
COMMENT     Sequence Label: pMpGWBs02
FEATURES             Location/Qualifiers
     source          1..11991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2927..3179
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3212..3228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3212..3228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3225..3247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3236..3252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3260..3266,3267..3284,3285..3290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3305..3326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3411..3585
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4642..5318)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5513..5537
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6058..6849
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7095..7683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7584..7603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7837..7854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7869..8009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7935..7954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8095..8117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8353..8547
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8613..9686)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10115..10744)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc gttcaaacat
     2941 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3001 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3061 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3121 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3181 ggaattggtt ccggaaccaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     3241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3301 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     3361 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     3421 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     3481 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     3541 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gctaggcatg
     3601 atctaaccct cggtctctgg cgtcgcgact gcgaaatttc gcgagggttt ccgagatggt
     3661 gattgcgctt cgcagatctc caggcgcgtg ggtgcggacg tagtcagcgc cattgccgat
     3721 cgcgtgaagt tccgccgcaa ggctcgctgg acccagatcc tttacaggaa ggccaacggt
     3781 ggcgcccaag aaggatttcc gcgacaccga gaccaatagc ggaagcccca acgccgactt
     3841 cagcttttga aggttcgaca gcacgtgcag cgatgtttcc ggtgcggggc tcaagaaaaa
     3901 tcccatcccc ggatcgagga tgagccggtc ggcagcgacc ccgctccgtc gcaaggcgga
     3961 aacccgcgcc tcgaagaacc gcacaatctc gtcgagcgcg tcttcgggtc gaaggtgacc
     4021 ggtgcgggtg gcgatgccat cccgctgcgc tgagtgcata accaccagcc tgcagtccgc
     4081 ctcagcaata tcgggataga gcgcagggtc aggaaatcct tggatatcgt tcaggtagcc
     4141 cacgccgcgc ttgagcgcat agcgctgggt ttccggttgg aagctgtcga ttgaaacacg
     4201 gtgcatctga tcggacaggg cgtctaagag cggcgcaata cgtctgatct catcggccgg
     4261 cgatacaggc ctcgcgtccg gatggctggc ggccggtccg acatccacga cgtctgatcc
     4321 gactcgcagc atttcgatcg ccgcggtgac agcgccggcg gggtctagcc gccggctctc
     4381 atcgaagaag gagtcctcgg tgagattcag aatgccgaac accgtcacca tgcactttac
     4441 tcttccacca ttgcttgtaa tggaagtaat gtcagtgttg accttcttaa ctgggaatcc
     4501 agtcatggat ttgaggccgc cgaatggagc caccgcggcc gattgcaccg tagaagcacg
     4561 gctgactgta gtcacagctg aagaggatat catagaagcc atctcgagga gagatagatt
     4621 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
     4681 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     4741 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     4801 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     4861 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     4921 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     4981 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     5041 gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg
     5101 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     5161 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     5221 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     5281 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     5341 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
     5401 gctctagcca atacgcaaac cgaattcaga aataaattca gcctaattcg gcgttaattc
     5461 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     5521 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     5581 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     5641 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     5701 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     5761 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     5821 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     5881 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     5941 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     6001 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     6061 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     6121 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     6181 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     6241 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     6301 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     6361 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     6421 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     6481 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     6541 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     6601 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     6661 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     6721 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     6781 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     6841 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     6901 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     6961 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     7021 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7081 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7141 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7201 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7261 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7321 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7381 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7441 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7501 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7561 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7621 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7681 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7741 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7801 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7861 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7921 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     7981 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     8041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     8161 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     8221 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     8281 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     8341 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     8401 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     8461 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     8521 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     8581 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     8641 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     8701 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     8761 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     8821 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     8881 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     8941 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     9001 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     9061 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     9121 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     9181 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     9241 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     9301 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     9361 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     9421 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     9481 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     9541 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     9601 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     9661 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     9721 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     9781 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     9841 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     9901 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     9961 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    10021 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    10081 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    10141 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    10201 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    10261 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    10321 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    10381 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    10441 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    10501 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    10561 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    10621 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    10681 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    10741 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    10801 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    10861 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    10921 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    10981 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    11041 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    11101 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    11161 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    11221 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    11281 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    11341 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    11401 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    11461 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    11521 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    11581 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    11641 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    11701 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    11761 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    11821 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    11881 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    11941 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc t
//
