LOCUS       sequence-444528-         557 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 
            132, 133, 157, 158.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL
  TITLE     Expanding the molecular grammar of polar residues and arginine in 
            FUS prion-like domain phase separation and aggregation.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 15:2024.02.15.580391. doi: 
            10.1101/2024.02.15.580391.
  PUBMED    38405719
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 15:2024.02.15.580391. doi: 
            10.1101/2024.02.15.580391."
COMMENT     Sequence Label: FUS LC 4QQ->NN
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggcttcaaa tgattataca cagcaagcaa ctcaaagcta cggcgcgtat ccgacccagc
      121 cgggtcaagg ttatagtaat aacagctcgc aaccgtatgg ccagcagagc tacagcggtt
      181 atagccagtc tactgacacc agcggttatg gtcaatcctc atacagctcc tacggccagt
      241 cccagaatac cggttacggc acccagtcca ccccgcaggg ttatggtagc acgggtggtt
      301 atggcagcag ccagtcctcc caatcttcgt acggcaacaa cagctcctac ccgggttatg
      361 gccaacaacc ggctccgagc tctacgagcg gtagctacgg cagctcttcg caatccagct
      421 cttacggcca acctcagtct ggtagctaca gcaataaccc gagctacggc ggtcagcaac
      481 aatcgtacgg ccagcaacag agctacaatc caccgcaggg ctatggtaac aacaaccagt
      541 ataacagctg actcgag
//
