LOCUS       sequence-445269-       11929 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Packaging plasmid for TadCBEd in v3 PE-eVLP architecture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: gag-TadCBEd
FEATURES             Location/Qualifiers
     source          1..11929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..420
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     350..372
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     388..404
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             649..675
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             688..714
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             727..753
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1831..1851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2449..2472,2473..2475,2476..6549)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2449..6549
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6580..6828
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6859..7107
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7150..7170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7176..7195)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7241..7296
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7295..7314)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7654..7670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7654..7670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7667..7689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7678..7694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7702..7708,7709..7726,7727..7732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7747..7768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7885..7902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8056..8644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8136..8155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8815..9606,9607..9675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9438..9457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9676..9780)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9806..10261)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9943..9964)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10155..10174
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10388..10410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10402..10419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10403..10419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10429..10448
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10429..10447
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        10568..10947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10948..11151
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11102..11122
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11148..11172
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          11263..11835
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
       61 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
      121 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
      181 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
      241 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
      301 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
      361 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      421 aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa
      481 gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga
      541 gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg
      601 agacgggagc cccctgtggc cgactccact tctggcggct cacttcaact gcctccactt
      661 gaaagactga cactgggatc attacaatta cctcctttag aacgattaac actcggttca
      721 ctacagcttc cgcctcttga gagattgaca ttaagtggtg gatctacctc gcaggcattc
      781 cccctccgcg caggaggaaa cggacagctt caatactggc cgttctcctc ttctgacctt
      841 tacaactgga aaaataataa cccttctttt tctgaagatc caggtaaact gacagctctg
      901 atcgagtctg tcctcatcac ccatcagccc acctgggacg actgtcagca gctgttgggg
      961 actctgctga ccggagaaga aaaacaacgg gtgctcttag aggctagaaa ggcggtgcgg
     1021 ggcgatgatg ggcgccccac tcaactgccc aatgaagtcg atgccgcttt tcccctcgag
     1081 cgcccagact gggattacac cacccaggca ggtaggaacc acctagtcca ctatcgccag
     1141 ttgctcctag cgggtctcca aaacgcgggc agaagcccca ccaatttggc caaggtaaaa
     1201 ggaataacac aagggcccaa tgagtctccc tcggccttcc tagagagact taaggaagcc
     1261 tatcgcaggt acactcctta tgaccctgag gacccagggc aagaaactaa tgtgtctatg
     1321 tctttcattt ggcagtctgc cccagacatt gggagaaagt tagagaggtt agaagattta
     1381 aaaaacaaga cgcttggaga tttggttaga gaggcagaaa agatctttaa taaacgagaa
     1441 accccggaag aaagagagga acgtatcagg agagaaacag aggaaaaaga agaacgccgt
     1501 aggacagagg atgagcagaa agagaaagaa agagatcgta ggagacatag agagatgagc
     1561 aagctattgg ccactgtcgt tagtggacag aaacaggata gacagggagg agaacgaagg
     1621 aggtcccaac tcgatcgcga ccagtgtgcc tactgcaaag aaaaggggca ctgggctaaa
     1681 gattgtccca agaaaccacg aggacctcgg ggaccaagac cccagacctc cctcctgacc
     1741 ctagatgacg gcggatccac gtccacgctg ctaatggaga actcgtctgg aggttctaaa
     1801 cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt cagttctgag
     1861 gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgg
     1921 gatgagagga aggcgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag
     1981 ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatagccctg
     2041 agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca
     2101 ttcgagcctt gcgtgatgtg cgccggcgcc atgatcaact ctaggatcgg ccgcgtggtg
     2161 tttggcgtga ggaactcaaa aagaggcgcc gcaggctccc tgatgaacgt gctgaactac
     2221 cccggaatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc
     2281 ctgctgtgcg atttctatcg gatgcctaga caggtgttca atgctcagaa gaaggcccag
     2341 agctccatca actctggcgg atctagcgga ggatcctctg gcagcgagac accaggaaca
     2401 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcga caagaagtac
     2461 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     2521 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     2581 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     2641 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     2701 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     2761 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     2821 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     2881 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     2941 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     3001 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     3061 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     3121 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     3181 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     3241 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     3301 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     3361 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     3421 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     3481 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     3541 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     3601 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     3661 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     3721 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     3781 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     3841 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     3901 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     3961 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     4021 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     4081 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     4141 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     4201 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     4261 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     4321 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     4381 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     4441 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     4501 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     4561 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     4621 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     4681 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     4741 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     4801 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     4861 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     4921 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     4981 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     5041 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     5101 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     5161 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     5221 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     5281 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     5341 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     5401 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     5461 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     5521 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     5581 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     5641 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     5701 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     5761 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     5821 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     5881 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     5941 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     6001 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     6061 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     6121 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     6181 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     6241 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     6301 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     6361 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     6421 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     6481 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     6541 ggaggtgaca gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt
     6601 gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
     6661 gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac
     6721 gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg
     6781 gccctggtca tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc
     6841 ggaggatctg gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag
     6901 ctggtcatcc aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac
     6961 aagcctgaga gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg
     7021 atgctgctga catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc
     7081 aacggagaga acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc
     7141 gaattcgagc ccaagaagaa gaggaaagtc tgagatcttt ttccctctgc caaaaattat
     7201 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     7261 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     7321 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     7381 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     7441 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     7501 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     7561 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     7621 tccctcgacg gatcggccgc aattcgtaac catgtcatag ctgtttcctg tgtgaaattg
     7681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7801 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7861 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8461 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9781 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     9841 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     9901 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     9961 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
    10021 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
    10081 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
    10141 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
    10201 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
    10261 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10321 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    10381 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
    10441 ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct tggcccattg
    10501 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
    10561 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
    10621 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
    10681 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
    10741 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    10801 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    10861 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    10921 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    10981 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    11041 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    11101 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
    11161 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
    11221 gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt ggggaccctt
    11281 gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc
    11341 agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt
    11401 tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt
    11461 tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt
    11521 gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt
    11581 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
    11641 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
    11701 tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
    11761 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
    11821 ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc
    11881 aaagaattcc tcgagggccg gatccgaatt cctgtatttg tctgaaaac
//
